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First report of the symbiotic bacterium Xenorhabdus indica associated with the entomopathogenic nematode Steinernema yirgalemense

Published online by Cambridge University Press:  14 August 2014

T. Ferreira ,
C.A. van Reenen ,
P. Tailliez ,
S. Pagès ,
A.P. Malan  and
L.M.T. Dicks
T. Ferreira
Affiliation:
Department of Conservation Ecology and Entomology, Stellenbosch University, Private Bag X1, 7602Matieland, Stellenbosch, South Africa
C.A. van Reenen
Affiliation:
Department of Microbiology, Stellenbosch University, Private Bag X1, 7602Matieland, Stellenbosch, South Africa
P. Tailliez
Affiliation:
Université Montpellier 2, UMR1333 Diversité, Génomes & Interactions Microorganismes-Insectes (DGIMI), F-34000Montpellier, France INRA, UMR1333 Diversité, Génomes & Interactions Microorganismes-Insectes (DGIMI), F-34000Montpellier, France
S. Pagès
Affiliation:
Université Montpellier 2, UMR1333 Diversité, Génomes & Interactions Microorganismes-Insectes (DGIMI), F-34000Montpellier, France INRA, UMR1333 Diversité, Génomes & Interactions Microorganismes-Insectes (DGIMI), F-34000Montpellier, France
A.P. Malan
Affiliation:
Department of Conservation Ecology and Entomology, Stellenbosch University, Private Bag X1, 7602Matieland, Stellenbosch, South Africa
L.M.T. Dicks*
Affiliation:
Department of Microbiology, Stellenbosch University, Private Bag X1, 7602Matieland, Stellenbosch, South Africa
*
* E-mail: lmtd@sun.ac.za
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Abstract

The entomopathogenic nematode Steinernema yirgalemense is considered a promising agent in the biocontrol of insects. However, little is known about the bacteria living in symbiosis with the nematode. In this study, we have identified the only available bacterial strain (157-C) isolated from S. yirgalemense, as a member of the species Xenorhabdus indica. Identification was based on 16S rDNA, recA, dnaN, gltX, gyrB and infB gene sequence analyses. The relatedness of strain 157-C to the type strain of X. indica (DSM 17 382) was confirmed with DNA–DNA hybridization. The phenotypic characteristics of strain 157-C are similar to those described for the type strain of X. indica. This is the first report associating X. indica with S. yirgalemense.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 1 , January 2016 , pp. 108 - 112
Copyright
Copyright © Cambridge University Press 2014 

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