Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-15T01:23:11.496Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic diversity of infective larvae of Gnathostoma spinigerum (Nematoda: Gnathostomatidae) in freshwater swamp eels from Thailand

Published online by Cambridge University Press:  28 November 2016

P. Eamsobhana ,
D. Wanachiwanawin ,
K. Roongruangchai ,
S.L. Song  and
H.S. Yong
P. Eamsobhana*
Affiliation:
Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
D. Wanachiwanawin*
Affiliation:
Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
K. Roongruangchai
Affiliation:
Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
S.L. Song
Affiliation:
Institute of Ocean and Earth Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia
H.S. Yong
Affiliation:
Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia
*
*E-mails: praphathip.eam@mahidol.ac.th; darawan.wan@mahidol.ac.th
*E-mails: praphathip.eam@mahidol.ac.th; darawan.wan@mahidol.ac.th
Get access Rights & Permissions [Opens in a new window]

Abstract

Human gnathostomiasis is a food-borne zoonosis caused by a tissue nematode of the genus Gnathostoma. The disease is highly endemic in Asia, including Thailand. The freshwater swamp eel (Monopterus albus), the second intermediate host of the gnathostome nematode, has an important role in transmitting the infection in Thailand. Surveys on the infective larvae of Gnathostoma spinigerum based on morphological features in freshwater swamp eels have been performed continuously and reported in Thailand. However, there is still limited molecular data on intra-species variations of the parasite. In this study, a total of 19 third-stage larvae of morphologically identified G. spinigerum were collected from 437 liver samples of freshwater swamp eels purchased from a large wholesale market in Bangkok, Thailand. Molecular characterization based on mitochondrial cytochrome c oxidase subunit I (COI) sequences was performed to elucidate their genetic variations and phylogenetic relationship. Among the 19 infective larvae recovered from these eels, 16 were sequenced successfully. Phylogenetic analyses inferred from the partial COI gene showed the presence of three distinct COI haplotypes. Our findings confirm the presence of G. spinigerum as the main species in Thailand.

Type
Short Communications
Information
Journal of Helminthology , Volume 91 , Issue 6 , November 2017 , pp. 767 - 771
Copyright
Copyright © Cambridge University Press 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ando, K., Tsunemori, M., Akahane, H., Tesana, S., Hasegawa, H. & Chinzei, Y. (2006) Comparative study on DNA sequences of ribosomal DNA and cytochrome c oxidase subunit 1 of mitochondrial DNA among five species of gnathostomes. Journal of Helminthology 80, 7–13.Google Scholar
Aozasa, N., Tsunemi, Y., Oto, Y., Kai, H., Morimura, S., Kakinuma, T., Tamaki, K., Sato, S., Maeda, T. & Yamasaki, H. (2011) A case of cutaneous larva migrans caused by Gnathostoma spinigerum . Hifu(ka No Rinsho 53, 887–890.Google Scholar
Bandelt, H.J., Forster, P. & Röhl, A. (1999) Median-joining networks for inferring intraspecific phylogenies. Molecular Biology Evolution 16, 37–48.Google Scholar
Daengsvang, S. (1980) A monograph on the genus Gnathostoma and gnathostomiasis in Thailand. Tokyo, Japan, Southeast Asian Medical Information Center (SEAMIC), International Medical Foundation of Japan.Google Scholar
Daengsvang, S. (1986) Gnathostoma spinigerum and human gnathostomiasis. pp. 124–147 in Sucharit, S. (Ed.) The 25th Anniversary of the Faculty of Tropical Medicine, Mahidol University. Bangkok, Thailand, Krung Siam Press.Google Scholar
Jongthawin, J., Intapan, P.M., Sanpool, O., Sadaow, L., Janwan, P., Thanchomnang, T., Sangchan, A., Visaetsilpanonta, S., Keawkong, W. & Maleewong, W. (2015) Three human gnathostomiasis cases in Thailand with molecular identification of causative parasite species. American Journal of Tropical Medicine and Hygiene 93, 615–618.CrossRefGoogle ScholarPubMed
Jongthawin, J., Intapan, P.M., Sanpool, O., Janwan, P., Sadaow, L., Thanchomnang, T., Laymanivong, S. & Maleewong, W. (2016) Molecular-phylogenetic confirmation of Gnathostoma spinigerum Owen, 1836 (Nematoda: Gnathostomatidae) from Lao PDR and Thailand. Folia Parasitologica 63, 002. doi: 10.14411/fp.2016.002.CrossRefGoogle Scholar
Nawa, Y., Maleewong, W., Intapan, P.M. & Diaz-Chamacho, S.P. (2015) Gnathostoma. pp. 405–426 in Xiao, L., Ryan, U. & Feng, Y. (Eds) Biology of food borne parasites. New York, CRC Press, Taylor and Francis Group.Google Scholar
Ngarmamonpirat, C., Waikagul, J., Petmitr, S., Dekumyoy, P., Rojekittikhun, W. & Anantapruti, M.T. (2005) Analysis of sequence variation in Gnathostoma spinigerum mitochondrial DNA by single-strand conformation polymorphism analysis and DNA sequence. Parasitology International 54, 65–68.Google Scholar
Nuamtanong, S., Waikagul, J. & Anantaphruti, M.T. (1998) Gnathostome infection in swamp eels, Fluta alba, in central Thailand. Southeast Asian Journal of Tropical Medicine and Public Health 29, 144–147.Google ScholarPubMed
Rojekittikhun, W., Pubampen, S. & Waikagul, J. (1988) Seasonal variation in the intensity of Gnathostoma larvae in swamp eels (Fluta alba) sold in a local market in Bangkok. Southeast Asian Journal of Tropical Medicine and Public Health 29, 148–153.Google Scholar
Rojekittikhun, W., Pubampen, S., Hiranyachattada, P., Chiamratana, B. & Setasuban, P. (1989) A survey on the infective larvae of Gnathostoma spinigerum in freshwater fish sold in the local markets of Bangkok. Journal of Tropical Medicine and Parasitology 12, 7–12.Google Scholar
Saksirisampant, W. & Thanomsub, B.W. (2012) Positivity and intensity of Gnathostoma spinigerum infective larvae in farmed and wild-caught swamp eels in Thailand. Korean Journal of Parasitology 50, 113–118.CrossRefGoogle ScholarPubMed
Saksirisampant, W., Kulkaew, K., Nuchprayoon, S., Yentakham, S. & Wiwanitkit, V. (2002) A survey of the infective larvae of Gnathostoma spinigerum in swamp eels bought in a local market in Bangkok, Thailand. Annals Tropical Medicine and Parasitolology 96, 191–195.Google Scholar
Setasuban, P., Nuamtanong, S., Rojanakittikoon, V., Yaemput, S., Dekumyoy, P., Akahane, H. & Kojima, S. (1991) Gnathostomiasis in Thailand: a survey on intermediate hosts of Gnathostoma spp. with special reference to a new type of larvae found in Fluta alba . Southeast Asian Journal of Tropical Medicine and Public Health 22 (Suppl.), 220–224.Google Scholar
Xuan, L.T. & Rojekittikhun, W. (2000) A survey of infective larvae of Gnathostoma in eels sold in Ho Chi Minh City. Southeast Asian Journal of Tropical Medicine and Public Health 31, 133–137.Google Scholar
Yong, H.S., Eamsobhana, P., Song, S.L., Prasartvit, A. & Lim, P.E. (2015) Molecular phylogeography of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae) and genetic relationships with congeners using cytochrome b gene marker. Acta Tropica 148, 66–71.CrossRefGoogle ScholarPubMed