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Immunosuppressive PAS-1 is an excretory/secretory protein released by larval and adult worms of the ascarid nematode Ascaris suum

Published online by Cambridge University Press:  07 April 2014

M.F.P. Antunes ,
T.O. Titz ,
I.F.C. Batista ,
R. Marques-Porto ,
C.F. Oliveira ,
C.A. Alves de Araujo  and
M.F. Macedo-Soares
M.F.P. Antunes
Affiliation:
Laboratory of Immunopathology, Butantan Institute, São Paulo, Brazil
T.O. Titz
Affiliation:
Laboratory of Immunopathology, Butantan Institute, São Paulo, Brazil
I.F.C. Batista
Affiliation:
Peptide and Protein Sequencing Unit, Butantan Institute, São Paulo, Brazil
R. Marques-Porto
Affiliation:
Peptide and Protein Sequencing Unit, Butantan Institute, São Paulo, Brazil
C.F. Oliveira
Affiliation:
Laboratory of Immunopathology, Butantan Institute, São Paulo, Brazil
C.A. Alves de Araujo*
Affiliation:
Laboratory of Immunopathology, Butantan Institute, São Paulo, Brazil
M.F. Macedo-Soares
Affiliation:
Laboratory of Immunopathology, Butantan Institute, São Paulo, Brazil
*
*E-mail: claudia.alvesdearaujo@yahoo.com; claudia.alvesdearaujo@stjude.org
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Abstract

Helminths use several strategies to evade and/or modify the host immune response, including suppression or inactivation of the host antigen-specific response. Several helminth immunomodulatory molecules have been identified. Our studies have focused on immunosuppression induced by the roundworm Ascaris suum and an A. suum-derived protein named protein 1 from A. suum (PAS-1). Here we assessed whether PAS-1 is an excretory/secretory (E/S) protein and whether it can suppress lipopolysaccharide-induced inflammation. Larvae from infective eggs were cultured in unsupplemented Dulbecco's modified Eagle medium (DMEM) for 2 weeks. PAS-1 was then measured in the culture supernatants and in adult A. suum body fluid at different time points by enzyme-linked immunosorbent assay (ELISA) with the monoclonal antibody MAIP-1. Secreted PAS-1 was detected in both larval culture supernatant and adult body fluid. It suppressed lipopolysaccharide (LPS)-induced leucocyte migration and pro-inflammatory cytokine production, and stimulated interleukin (IL)-10 secretion, indicating that larval and adult secreted PAS-1 suppresses inflammation in this model. Moreover, the anti-inflammatory activity of PAS-1 was abolished by treatment with MAIP-1, a PAS-1-specific monoclonal antibody, confirming the crucial role of PAS-1 in suppressing LPS-induced inflammation. These findings demonstrate that PAS-1 is an E/S protein with anti-inflammatory properties likely to be attributable to IL-10 production.

Type
Research Papers
Information
Journal of Helminthology , Volume 89 , Issue 3 , May 2015 , pp. 367 - 374
Copyright
Copyright © Cambridge University Press 2014 

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Footnotes

†

Current address: St Jude Children's Research Hospital, 262 Danny Thomas Place, Room D5055, Mail Stop 321, Memphis, TN 38 105 USA.

‡

These authors contributed equally to this work.

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