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The source, release and specificity of proteolytic enzyme activity produced by Anisakis simplex larvae (Nematoda: Ascaridida) in vitro

Published online by Cambridge University Press:  05 June 2009

Bernard E. Matthews
Affiliation:
Department of Zoology, University of Edinburgh, West Mains Road, Edinburgh E119 3JT, U.K.

Abstract

Indirect fluorescent antibody techniques and incubation of sectioned and ligatured larvae have been used to locate the source of azocoll-positivc secretions within the oesophagcal glands of third-stage Anisakis simplex larvae. The “excretory” system, previously suggested as a source of invasive enzymes, docs not seem to be involved. The proteolytic enzyme has trypsin-like properties and evidence is presented for its continuous synthesis during in vitro storage.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1984

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