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Trichrome staining of Gyrodactylus sclerites and soft tissues following fixation in ammonium picrate-glycerin, including an improved rendition of the haptoral bars of G. turnbulli

Published online by Cambridge University Press:  05 June 2009

G.R. Richards  and
J.C. Chubb
G.R. Richards
Affiliation:
Department of Environmental and Evolutionary Biology, University of Liverpool, Derby Building, PO Box 147, Liverpool, L69 3 BX, UK
J.C. Chubb
Affiliation:
Department of Environmental and Evolutionary Biology, University of Liverpool, Derby Building, PO Box 147, Liverpool, L69 3 BX, UK
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Abstract

A simple technique using modified Mallory stain in the transferral of Gyrodactylus specimens from ammonium picrate-glycerin to a permanent mountant is described. Hamuli, their connecting bars and the penis sclerites are well defined by the technique as are muscles and tendons, cell nuclei, tegument and gland cells. As well as being useful in the study of general anatomy, the technique enhances the observation of the taxonomically important ventral and dorsal bars. In order to show this, improved illustrations of the dorsal and ventral bars of G. turnbulli are given along with explicit demonstrations of differences in morphology of the ventral bars of G. bullatarudis and G. rasini - two easily confused species.

Type
Research Papers
Information
Journal of Helminthology , Volume 69 , Issue 2 , June 1995 , pp. 149 - 154
Copyright
Copyright © Cambridge University Press 1995

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