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The use of video-imaging to assesss the sub-lethal impact of plant secondary compounds on Schistosoma mansoni miracidia

Published online by Cambridge University Press:  05 June 2009

J.R.A. Lyddiard ,
A. Bartlett ,
B. Gray  and
P.J. Whitfield
J.R.A. Lyddiard*
Affiliation:
Infection and Immunity Research Group, Division of Life Sciences, King's College London, University of London, Campden Hill Road, Kensington, London, W8 7AH, UK
A. Bartlett
Affiliation:
Faculty of Science, Design and Technology, NESCOT, Reigate Road, Ewell, Surrey, KT7 3DF, UK
B. Gray
Affiliation:
Faculty of Science, Design and Technology, NESCOT, Reigate Road, Ewell, Surrey, KT7 3DF, UK
P.J. Whitfield
Affiliation:
Infection and Immunity Research Group, Division of Life Sciences, King's College London, University of London, Campden Hill Road, Kensington, London, W8 7AH, UK
*
*Fax: 0171 333 5454. E-mail: james.r.lyddiard@kcl.ac.uk
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Abstract

The study describes methods developed for using video-imaging technology to record and measure the velocity of Schistosoma mansoni miracidia. The efficacy of the classical bioassay procedure (a qualitative behavioural assay) was compared with that of the new quantitative protocol, for assessing the sub-lethal impact of a larvicidal dichloromethane extract of the seeds of Millettia thonningii on miracidia. The new technique confirmed the efficacy of the classical bioassay for rapid determination of the lethal and sub-lethal impact of larvicides but also provided quantitative information on sub-lethal impacts on miracidial velocity and shape.

Type
Research Papers
Information
Journal of Helminthology , Volume 72 , Issue 3 , September 1998 , pp. 237 - 241
Copyright
Copyright © Cambridge University Press 1998

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