Hostname: page-component-78c5997874-v9fdk Total loading time: 0 Render date: 2024-11-14T04:25:02.141Z Has data issue: false hasContentIssue false
  • English
  • Français

Adherence of carp leucocytes to adults and cercariae of the blood fluke Sanguinicola inermis

Published online by Cambridge University Press:  05 June 2009

D.T. Richards ,
D. Hoole ,
J.W. Lewis ,
E. Ewens  and
C. Arme
D.T. Richards
Affiliation:
Parasitology Research Laboratory, Centre for Applied Entomology & Parasitology, Department of Biological Sciences, Keele University, Keele, Staffordshire, ST5 5BG, UK
D. Hoole
Affiliation:
Parasitology Research Laboratory, Centre for Applied Entomology & Parasitology, Department of Biological Sciences, Keele University, Keele, Staffordshire, ST5 5BG, UK
J.W. Lewis
Affiliation:
School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, TW20 OEX, UK
E. Ewens
Affiliation:
School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, TW20 OEX, UK
C. Arme
Affiliation:
Parasitology Research Laboratory, Centre for Applied Entomology & Parasitology, Department of Biological Sciences, Keele University, Keele, Staffordshire, ST5 5BG, UK
Get access Rights & Permissions [Opens in a new window]

Abstract

Live adult and cercarial stages of Sanguinicola inermis Plehn, 1905 (Trematoda: Sanguinicolidae) were maintained in vitro in the presence of carp (Cyprinus carpio L.) leucocytes. Cells and parasites were fixed at intervals from 0.25 to 48 h and examined using light microscopy, SEM and TEM. Within 12 h of exposure, leucocytes were found attached to cercariae although, by 24 h, fewer cells were found attached to postcercarial, juvenile adult stages that had shed their tails. Neutrophils and macrophages were found attached to the damaged tegument of cercariae that had not transformed by 48 h. Few cells were attached to the tegument of adult flukes that were alive when fixed. However, there was extensive tegumental damage and numerous cells were attached to adult flukes that had died before fixation. The results are discussed with reference to parasite survival within the vascular system of the host.

Type
Research Papers
Information
Journal of Helminthology , Volume 70 , Issue 1 , March 1996 , pp. 63 - 67
Copyright
Copyright © Cambridge University Press 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Brink, L.H., McLaren, D.J. & Smithers, S.R. (1977) Schistosoma mansoni: a comparative study of artificially-transfonned schistosomula and schistosomula recovered after cercarial penetration of isolated skin. Parasitology 74, 73–86.Google Scholar
Butterworth, A.C. (1984) Cell mediated damage to helminths. Advances in Parasitology 23, 143–235CrossRefGoogle ScholarPubMed
Capron, M., Rousseaux, J., Mazingue, C., Bazin, H. & Capron, A. (1978) Rat mast cell-eosinophil interaction in antibody-dependent eosinophil cytotoxicity to Schistosoma mansoni schistosomula. Journal of Immunology 121, 2518–2525.Google Scholar
Cenini, , (1984) The ultrastructure of leucocytes in carp (Cyprinus carpio). Journal of Zoology 204, 509–520.Google Scholar
Fuller, D.W., Pilcher, K.S. & Fryer, J.L. (1977) A leukocytolytic factor isolated from cultures of Aeromonas salmonicida. Journal of the Fisheries Board of Canada 34, 1118–1125.Google Scholar
Hoole, D. & Arme, C. (1986) The role of serum in leucocyte adherence to the plerocercoid of Ligula intestinalis (Cestoda: Pseudophyllidea). Parasitology 92, 413–424.Google Scholar
Kirk, R.S. & Lewis, J.W. (1992) The laboratory maintenance of Sanguinicola inermis Plehn, 1905 (Digenea: Sanguinicolidae). Parasitology 104, 121–127.Google Scholar
Kirk, R.S. & Lewis, J.W. (1993) The life-cycle and morphology of Sanguinicola inermis Plehn, 1905 (Digenea: Sanguinicolidae) Systematic Parasitology 25, 125–133.Google Scholar
Lee, R.S. (1990) The development of Sanguinicola inermis Plehn, 1905 (Digenea: Sanguinicolidae) in the common carp Cyprinus carpio L. PhD Thesis, Royal Holloway and Bedford New College, University of London.Google Scholar
McLaren, D.J. (1980) Schistosoma mansoni: The parasite surface in relation to host immunity. Chichester, Research Studies Press.Google Scholar
McLaren, D.J. & Terry, R.J. (1982) The protective role of acquired host antigens during schistosome maturation. Parasite Immunology 4, 129–148.CrossRefGoogle ScholarPubMed
McLaren, D.J., Clegg, J.A. & Smithers, R. (1975) Acquisition of host antigens by young Schistosoma mansoni in mice: correlation with failure to bind antibody in vivo. Parasitology 70, 67–75.Google Scholar
Ramalho-Pinto, F.J., McLaren, D.J. & Smithers, S.R. (1978) Complement-mediated killing, of schistosomula of Schistosoma mansoni by rat eosinophils in vitro. Journal of Experimental Medicine 147, 147–156.Google Scholar
Ramalho-Pinto, F.J., De Rossi, R. & Smithers, S.R. (1979) Murine schistosomiasis mansoni: anti-schistosomula antibodies and the IgG subclasses involved in the complement- and eosinophil-mediated killing of schistosomula in vitro. Parasite Immunology 1, 295–308.CrossRefGoogle ScholarPubMed
Richards, D.T., Hoole, D., Lewis, J.W., Ewens, E. & Arme, C. (1994a) Ultrastructural observations on the cellular response of carp (Cyprinus carpio L.) to eggs of the blood fluke Sanguinicola inermis Plehn, 1905 (Trematoda: Sanguinicolidae). Journal of Fish Diseases 17, 439–446.Google Scholar
Richards, D.T., Hoole, D., Lewis, J.W., Ewens, E. & Arme, C. (1994b) Changes in the cellular composition of the spleen and pronephros of carp Cyprinus carpio infected with the blood fluke Sanguinicola inermis (Trematoda: Sanguinicolidae). Diseases of Aquatic Organisms 19, 173–179.Google Scholar
Richards, D.T., Hoole, D., Lewis, J.W., Ewens, E. & Arme, C. (in press) Stimulation of carp Cyprinus carpio lymphocytes in vitro by the blood fluke Sanguinicola inermis (Trematoda: Sanguinicolidae). Diseases of Aquatic Organisms.Google Scholar
Richards, D.T., Hoole, D., Lewis, J.W., Ewens, E. & Arme, C. (in press) In vitro polarisation of carp (Cyprinus carpio L.) leucocytes in response to the blood fluke Sanguinicola inermis Plehn, 1905 (Trematoda: Sanguinicolidae). Parasitology.Google Scholar
Sharp, G.J.E., Pike, A.W. & Secombes, C.J. (1991) Rainbow trout [Oncorhynchus mykiss (Walbaum, 1792)] leucocyte interactions with metacestode stages of Diphyllobothrium dendriticum (Nitzsch, 1824), (Cestoda, Pseudophyllidea). Fish and Shellfish Immunology 1, 195–211.CrossRefGoogle Scholar
Sher, A., Hall, B.F. & Vadas, M. A. A. (1978) Acquisition of murine major histocompatibility complex gene products by schistosomula of Schistosoma mansoni. Journal of Experimental Medicine 148, 46–50.CrossRefGoogle ScholarPubMed
Smithers, S.R. & Terry, R.J. (1976) The immunology of schistosomiasis. Advances in Parasitology 14, 399–422.CrossRefGoogle ScholarPubMed
Sommerville, C. & Iqbal, N.A.M. (1991) The process of infection, migration, growth and development of Sanguinicola inermis Plehn, 1905 (Digenea: Sanguinicolidae) in carp, Cyprinus carpio L. Journal of Fish Diseases 14, 211–219.Google Scholar
Taylor, M.J. & Hoole, D. (1993) Ligula intestinalis (L.) (Cestoda: Pseudophyllidea): polarizarion of cyprinid leucocytes as an indicator of host- and parasite-derived chemo-attractants. Parasitology 107, 433–440.CrossRefGoogle Scholar
Torpier, G., Capron, A. & Ouassi, M. (1979) Receptor for IgG (Fc) and human β-microglobulin on S. mansoni schistosomula. Nature 278, 447–449.Google Scholar
Wiest, P.M., Kossmann, R.J. & Tartakoff, A.M. (1989) Determinants of surface membrane maturation during the cercarial-schistosomula transformation of Schistosoma mansoni. American Journal of Tropical Medicine and Hygiene 41, 70–77.CrossRefGoogle ScholarPubMed