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Analysis of polyunsaturated fatty acid composition of Strongyloides ratti in relation to development

Published online by Cambridge University Press:  05 June 2009

Takeya Minematsu ,
Seietsu Yamazaki ,
Yoshinori Uji ,
Hiroaki Okabe ,
Masataka Korenaga  and
Isao Tada
Takeya Minematsu
Affiliation:
Department of Parasitic Diseases, Kumamoto University Medical School, 2-2-1 Honjo, Kumamoto 860, Japan
Seietsu Yamazaki
Affiliation:
Analytical Chemistry Group, Tsukuba Research Center, Mitsubishi Petrochemical Co., Ltd. 8-3-1, Chuo, Ami-machi, Inashikigun, Ibaraki 300-03, Japan
Yoshinori Uji
Affiliation:
Laboratory Medicine, Kumamoto University Medical School, 2-2-1 Honjo, Kumamoto 860, Japan
Hiroaki Okabe
Affiliation:
Laboratory Medicine, Kumamoto University Medical School, 2-2-1 Honjo, Kumamoto 860, Japan
Masataka Korenaga
Affiliation:
Department of Parasitic Diseases, Kumamoto University Medical School, 2-2-1 Honjo, Kumamoto 860, Japan
Isao Tada
Affiliation:
Department of Parasitic Diseases, Kumamoto University Medical School, 2-2-1 Honjo, Kumamoto 860, Japan
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Abstract

The effect of linolenic acid (C18:3ω3) on the development of Strongyloides ratti first-stage larvae (L1) in culture was studied. The fatty acid composition of S. ratti free-living generations was analyzed by gas chromatography. L1 had abundant linoleic acid (C18:2ω6) but its proportion decreased with development. On the contrary, eicosapentaenoic acid (C20:5ω3) and C20:4ω3 were prominent in the filariform larva (L3). Because C20:5ω3 is generally synthesized from C18:3ω3 via C20:4ω3, the high ratio of C20:5ω3/C18:3ω3 of L3 in all the free-living generations suggested that polyunsaturated fatty acid metabolism, particularly the ω-3 series, and eicosanoids produced had important roles in the development of S. ratti L1.

Keywords

Strongyloides rattifatty acidslinolenic acidlinoleic acideicosapentaenoic acideicosanoidsdevelopment
Type
Research Article
Information
Journal of Helminthology , Volume 64 , Issue 4 , December 1990 , pp. 303 - 309
Copyright
Copyright © Cambridge University Press 1990

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