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Antigenicity of a proteolytic enzyme of Fasciola hepatica

Published online by Cambridge University Press:  05 June 2009

G. C. Coles  and
D. Rubano
G. C. Coles
Affiliation:
Department of Zoology, University of Massachusetts, Amherst, MA 01003–0027, USA
D. Rubano
Affiliation:
Department of Zoology, University of Massachusetts, Amherst, MA 01003–0027, USA
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Abstract

The possibility was examined of using a haemoglobinase released during in vitro incubation of adult Fasciola hepatica for immunodiagnosis of fascioliasis. By SDS gel electrophoresis the enzyme appeared as two closely migrating bands with a molecular weight of approximately 27 000 daltons. After Western blotting the bands reacted with serum from rats infected with F. hepatica and mice infected with Schistosoma mansoni. The enzyme was therefore not a good antigen for immunodiagnosis.

Keywords

Fasciola hepaticaSchistosoma mansoniproteolytic enzymeantigen
Type
Research Papers
Information
Journal of Helminthology , Volume 62 , Issue 3 , September 1988 , pp. 257 - 260
Copyright
Copyright © Cambridge University Press 1988

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References

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