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Chitin synthesis in zygotes of Ascaris suum

Published online by Cambridge University Press:  05 June 2009

P. Dubinský ,
M. Ryboš ,
L. Turčeková  and
E. Ossikovski
P. Dubinský
Affiliation:
Helminthological Institute of the Slovak Academy of Sciences, Dukelskych hrdinov 3, 040 01 KoSicc Czechoslovakia
M. Ryboš
Affiliation:
Helminthological Institute of the Slovak Academy of Sciences, Dukelskych hrdinov 3, 040 01 KoSicc Czechoslovakia
L. Turčeková
Affiliation:
Helminthological Institute of the Slovak Academy of Sciences, Dukelskych hrdinov 3, 040 01 KoSicc Czechoslovakia
E. Ossikovski
Affiliation:
Central Laboratory of Helminthology, Bulgarian Academy of Sciences, BontchevStr. 25 1113 Sofia, Bulgaria
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Abstract

In Ascaris suum chitin is formed in the zygote immediately after oocyte fertilization, and its synthesis is completed in the eggs from the distal half of the uterus. Incorporation of radiocarbon [C] glucose into chitin of the eggshell was 40-fold higher than incorporation of [C] glucosamine. The same rank order also holds for the incorporation of label from these isotopes into the glycogen of the ovaries. A large part of the radiolabel was incorporated first into oocyte glycogen and only after fertilization was it incorporated into eggshell chitin. Actinomycin D inhibited chitin synthesis in the eggs from the distal half of the uterus and it significantly reduced incorporation of radiocarbon from glucose into chitin.

Keywords

Ascaris suumchitinzygoteradiolabelling
Type
Research Article
Information
Journal of Helminthology , Volume 60 , Issue 3 , September 1986 , pp. 187 - 192
Copyright
Copyright © Cambridge University Press 1986

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