Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-13T08:09:21.773Z Has data issue: false hasContentIssue false
  • English
  • Français

Description and phylogenetic analyses of ribosomal transcription units from species of Fasciolidae (Platyhelminthes: Digenea)

Published online by Cambridge University Press:  06 March 2020

T.H. Le Open the ORCID record for T.H. Le [Opens in a new window] ,
K.L.T. Pham ,
H.T.T. Doan ,
T.K. Xuyen Le ,
K.T. Nguyen  and
S.P. Lawton
T.H. Le*
Affiliation:
Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), 18. Hoang Quoc Viet Rd, Cau Giay, Hanoi, Vietnam Graduate University of Science and Technology (GUST), Vietnam Academy of Science and Technology (VAST), 18. Hoang Quoc Viet Rd, Cau Giay, Hanoi, Vietnam
K.L.T. Pham
Affiliation:
Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), 18. Hoang Quoc Viet Rd, Cau Giay, Hanoi, Vietnam
H.T.T. Doan
Affiliation:
Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), 18. Hoang Quoc Viet Rd, Cau Giay, Hanoi, Vietnam Graduate University of Science and Technology (GUST), Vietnam Academy of Science and Technology (VAST), 18. Hoang Quoc Viet Rd, Cau Giay, Hanoi, Vietnam
T.K. Xuyen Le
Affiliation:
Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), 18. Hoang Quoc Viet Rd, Cau Giay, Hanoi, Vietnam
K.T. Nguyen
Affiliation:
Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), 18. Hoang Quoc Viet Rd, Cau Giay, Hanoi, Vietnam
S.P. Lawton
Affiliation:
Molecular Parasitology Laboratory, School of Life Sciences, Pharmacy and Chemistry, Kingston University London, Kingston Upon Thames, SurreyKT12 2EE, UK
*
Author for correspondence: T.H. Le, E-mail: imibtvn@gmail.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Many members of Fasciolidae are common trematodes in cattle, buffaloes, sheep, elephants, pigs, with some capable of infecting humans also. In this study, the complete or near-complete sequences of ribosomal transcription unit (rTU or rDNA), each of Fasciola hepatica (Australia), Fascioloides jacksoni (Sri Lanka), Fasciolopsis buski (Vietnam) and three isolates of F. gigantica (Vietnam), were obtained and characterized. The full length of rDNA for each F. hepatica, ‘hybrid’ Fasciola sp., Fas. jacksoni and Fa. Buski, was 7657 bp, 7966 bp, 7781 bp and 8361 bp, with the complete intergenic spacer region (IGS) (862 bp, 1170 bp, 987 bp and 561 bp), respectively. The rDNA of two ‘pure’ F. gigantica isolates from Vietnam was 6794 bp with unsequenced IGS. For 28S rRNA genes the Fasciola spp. are equal, 1958 bp for 18S, 160 bp for 5.8S, 3863 bp and 454 bp for ITS1 but ITS2 differ by one nucleotide (Thymine) (359 or 360 bp). The ITS1 of the sensu lato Fa. buski has some distinguishable features, 286 bp for ITS2, 3862 bp for 28S and four repeat units of 356–361 bp each found in ITS1. The 28S rDNA analysis showed the lowest level of divergence (0–0.57%) between F. hepatica and F. gigantica and higher (2.23–2.62%) and highest (6–6.42%) for Fas. jacksoni and Fasciolopsis, respectively. The tree of 43 strains/species clearly produced a well-supported phylogeny, where 18 fasciolids consistently grouped, forming a discrete Fasciolidae clade, distinct from Philophthalmidae, Echinostomatidae and Echinochasmidae in Echinostomatoidea. Fascioloides jacksoni is outside Fasciola spp.: basal with Fas. magna, as previously demonstrated.

Keywords

Fasciola spp.FasciolidaeIGSphylogenyrDNA sequenceribosomal transcription unit
Type
Short Communication
Information
Journal of Helminthology , Volume 94 , 2020 , e136
Copyright
Copyright © The Author(s) 2020. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Benson, G (1999) Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Research 27, 573–580.CrossRefGoogle ScholarPubMed
Blair, D (2006) Ribosomal DNA variation in parasitic flatworms. pp. 96–123in Maule, A (Ed.) Parasitic Flatworms: Molecular Biology, Biochemistry, Immunology and Control. CAB International, Oxfordshire, UK.Google Scholar
Briscoe, AG, Bray, RA, Brabec, J and Littlewood, DTJ (2016) The mitochondrial genome and ribosomal operon of Brachycladium goliath (Digenea: Brachycladiidae) recovered from a stranded Minke whale. Parasitology International 65, 271–275.CrossRefGoogle ScholarPubMed
Cerqueira, AV and Lemos, B (2019) Ribosomal DNA and the nucleolus as keystones of nuclear architecture, organization, and function. Trends in Genetics 35, 710–723.CrossRefGoogle ScholarPubMed
Heneberg, P (2013) Phylogenetic data suggest the reclassification of Fasciola jacksoni (Digenea: Fasciolidae) as Fascioloides jacksoni comb. nov. Parasitology Research 112, 1679–1689.CrossRefGoogle ScholarPubMed
Ichikawa, M and Itagaki, T (2010) Discrimination of the ITS1 types of Fasciola spp. based on a PCR-RFLP method. Parasitology Research 106, 757–761.CrossRefGoogle ScholarPubMed
Králová-Hromadová, I, Spakulová, M, Horácková, E, et al. (2008) Sequence analysis of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae): intraspecific variation and differentiation from Fasciola hepatica. Journal of Parasitology 94, 58–67.CrossRefGoogle ScholarPubMed
Kumar, S, Stecher, G and Tamura, K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33, 1870–1874.CrossRefGoogle ScholarPubMed
Le, TH, Blair, D and McManus, DP (2001) Complete DNA sequence and gene organization of the mitochondrial genome of the liverfluke, Fasciola hepatica L. (Platyhelminthes; Trematoda). Parasitology 123, 609–621.CrossRefGoogle Scholar
Le, TH, Nguyen, VD, Phan, BU, Blair, D and McManus, DP (2004) Case report: unusual presentation of Fasciolopsis buski in a Vietnamese child. Transactions of the Royal Society of Tropical Medicine and Hygiene 98, 193–194.CrossRefGoogle Scholar
Le, TH, De, NV, Agatsuma, T, Nguyen, TGT, Nguyen, QD, McManus, DP and Blair, D (2008) Human fascioliasis and the presence of hybrid/introgressed forms of Fasciola hepatica and Fasciola gigantica in Vietnam. International Journal for Parasitology 38, 725–730.CrossRefGoogle ScholarPubMed
Le, TH, Nguyen, NTB, Nguyen, KT, Doan, HTT, Dung, DT and Blair, D (2016) A complete mitochondrial genome from Echinochasmus japonicus supports the elevation of Echinochasminae Odhner, 1910 to family rank (Trematoda: Platyhelminthes). Infection, Genetics and Evolution 45, 369–377.CrossRefGoogle Scholar
Le, TH, Nguyen, KT, Nguyen, NTB, Doan, HTT, Dung, DT and Blair, D (2017) The ribosomal transcription units of Haplorchis pumilio and H. taichui and the use of 28S sequences for phylogenetic identification of common heterophyids in Vietnam. Parasites & Vectors 10, 17.CrossRefGoogle ScholarPubMed
Liu, GH, Gasser, RB, Young, ND, Song, HQ, Ai, L and Zhu, XQ (2014) Complete mitochondrial genomes of the ‘intermediate form’ of Fasciola and Fasciola gigantica, and their comparison with F. hepatica. Parasites & Vectors 7, 150.CrossRefGoogle ScholarPubMed
Lockyer, AE, Olson, PD, Ostergaard, P, et al. (2003) The phylogeny of the Schistosomatidae based on three genes with emphasis on the interrelationships of Schistosoma Weinland, 1858. Parasitology 126, 203–224.CrossRefGoogle ScholarPubMed
Lotfy, WM, Brant, SV, DeJong, RJ, Le, TH, Demiaszkiewicz, A, Rajapakse, RPVJ, Perera, VBVP, Laursen, JR and Loker, ES (2008) Evolutionary origins, diversification, and biogeography of liver flukes (Digenea, Fasciolidae). American Journal of Tropical Medicine and Hygiene 79, 248–255.CrossRefGoogle Scholar
Ma, J, He, JJ, Liu, GH, Leontovyč, R, Kašný, M and Zhu, XQ (2016) Complete mitochondrial genome of the giant liver fluke Fascioloides magna (Digenea: Fasciolidae) and its comparison with selected trematodes. Parasites & Vectors 9, 429.CrossRefGoogle ScholarPubMed
Ma, J, Sun, MM, He, JJ, Liu, GH, Ai, L, Chen, MX and Zhu, XQ (2017) Fasciolopsis buski (Digenea: Fasciolidae) from China and India may represent distinct taxa based on mitochondrial and nuclear ribosomal DNA sequences. Parasites & Vectors 10, 101.CrossRefGoogle ScholarPubMed
Mas-Coma, S, Valero, MA and Bargues, MD (2019) Fascioliasis. pp. 71–103in Toledo, R and Fried, B (Eds) Digenetic Trematodes. Advances in Experimental Medicine and Biology Vol. 1154. Springer International Publishing, Springer Nature Switzerland AG.CrossRefGoogle Scholar
Nguyen, NTB, De, NV, Nguyen, TKL, Quang, HH, Doan, HTT, Agatsuma, T and Le, TH (2018) Distribution status of hybrid types in large liver flukes, Fasciola species (Digenea: Fasciolidae), from ruminants and humans in Vietnam. Korean Journal of Parasitology 56, 453–461.CrossRefGoogle Scholar
Olson, PD, Cribb, TH, Tkach, VV, Bray, RA and Littlewood, DTJ (2003) Phylogeny and classification of the Digenea (Platyhelminthes: Trematoda). International Journal for Parasitology 33, 733–755.CrossRefGoogle Scholar
Periago, MV, Valero, MA, El Sayed, M, Ashrafi, K, El Wakeel, A, Mohamed, MY, Desquesnes, M, Curtale, F and Mas-Coma, S (2008) First phenotypic description of Fasciola hepatica/Fasciola gigantica intermediate forms from the human endemic area of the Nile Delta, Egypt. Infection, Genetics and Evolution 8, 51–58.CrossRefGoogle ScholarPubMed
Qiu, YY, Gao, Y, Li, Y, Ma, XX, Lv, QB, Hu, Y, Qiu, HY, Chang, QC and Wang, CR (2019) Comparative analyses of complete ribosomal DNA sequences of Clonorchis sinensis and Metorchis orientalis: IGS sequences may provide a novel genetic marker for intraspecific variation. Infection, Genetics and Evolution 78, 104–125.Google ScholarPubMed
Rajapakse, RPVJ, Lawton, SP, Karunathilake, KJK, Perera, VBVP, Nguyen, NTB and Le, TH (2019) Molecular characterization of Fasciola jacksoni from wild elephants (Elephas maximus maximus) of Sri Lanka; a taxonomic evaluation. Parasitology 146, 1247–1255.CrossRefGoogle ScholarPubMed
Su, X, Zhang, Y, Zheng, X, Wang, XX, Li, Y, Li, Q and Wang, CR (2018) Characterization of the complete nuclear ribosomal DNA sequences of Eurytrema pancreaticum. Journal of Helminthology 92, 484–490.CrossRefGoogle ScholarPubMed
Tatonova, YV, Chelomina, GN and Besprosvannykh, VV (2012) Genetic diversity of nuclear ITS1-5.8S-ITS2 rDNA sequence in Clonorchis sinensis Cobbold, 1875 (Trematoda: Opisthorchidae) from the Russian Far East. Parasitology International 61, 664–674.CrossRefGoogle ScholarPubMed
Tkach, VV, Kudlai, O and Kostadinova, A (2016) Molecular phylogeny and systematics of the Echinostomatoidea Looss, 1899 (Platyhelminthes: Digenea). International Journal for Parasitology 46, 171–185.CrossRefGoogle Scholar
Weider, LJ, Elser, JJ, Crease, TJ, Mateos, M, Cotner, JB and Markow, TA (2005) The functional significance of ribosomal rDNA variation: Impacts on the evolutionary ecology of organisms. Annual Review of Ecology, Evolution, and Systematics 36, 219–242.CrossRefGoogle Scholar
Zhao, GH, Blair, D, Li, XY, et al. (2011) The ribosomal intergenic spacer (IGS) region in Schistosoma japonicum: structure and comparisons with related species. Infection, Genetics and Evolution 11, 610–617.CrossRefGoogle ScholarPubMed
Zheng, X, Chang, QC, Zhang, Y, Tian, SQ, Lou, Y, Duan, H, Guo, DH, Wang, CR and Zhou, XQ (2014) Characterization of the complete nuclear ribosomal DNA sequences of Paramphistomum cervi. Scientific World Journal 2014, 751907.CrossRefGoogle ScholarPubMed
Supplementary material: File

Le et al. supplementary material

Le et al. supplementary material

Download Le et al. supplementary material(File)
File 51.1 KB