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Diagnostic Mr 31/32000 proteins of Schistosoma mansoni (Sm31/32) and S. haematobium (Sh31/32): stability and reaction conditions for prospective field tests

Published online by Cambridge University Press:  05 June 2009

Mohamed A. Idris  and
Andreas Ruppel
Mohamed A. Idris
Affiliation:
Institute for Tropical Hygiene, University of Heidelberg, Federal Republic of Germany
Andreas Ruppel*
Affiliation:
Institute for Tropical Hygiene, University of Heidelberg, Federal Republic of Germany
*
**Correspondence: Dr A. Ruppel, Institut für Tropenhygiene, Im Neuenheimer Feld 324, D-6900 Heidelberg, F.R.G.
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Abstract

The existence of adult Schistosoma haematobium worm proteins (Sh31/32) homologous to the diagnostic Sm31/32 of S. mansoni is shown. Sm31/32 and Sh31/32, adsorbed on nitrocellulose and kept dry on the bench before immunoblot analysis, were antigenically stable for at least 4 years including storage for 17 months in tropical climates. The antigens react with patient sera in the absence of defined buffers under rather simple conditions (“humid chamber blot”). The results add to the use of these antigens for serodiagnosis of schistosomiasis in endemic areas.

Keywords

diagnostic proteinsstabilityreaction conditionsSchistosoma haematobiumSh31/32Schistosoma mansoniSm31/32
Type
Research Papers
Information
Journal of Helminthology , Volume 65 , Issue 2 , June 1991 , pp. 89 - 94
Copyright
Copyright © Cambridge University Press 1991

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References

REFERENCES

Chappell, C. L. & Dresden, M. H. (1987a) Purification of cysteine proteinases from adult Schistosoma mansoni. Archives of Biochemistry and Biophysics, 256, 560–568.Google Scholar
Chappell, C. L. & Dresden, M. H. (1987b) Schistosoma mansoni adult worm proteinase: tissue localization in the parasite and antibody response in infected animals. In: Molecular Strategies of Parasite Invasion. New York: A. R. Liss. 309–408.Google Scholar
Chappell, C. L., Dresden, M. H., Gryseels, B. & Deelder, A. M. (1990) Antibody response to Schistosoma mansoni adult worm cysteine proteinases in infected individuals. American Journal of Tropical Medicine and Hygiene, 42, 335–341.Google Scholar
Davis, A. H., Nanduri, J. & Watson, D. C. (1987) Cloning and gene expression of Schistosoma mansoni protease. Journal of Biological Chemistry, 262, 12851–12855.CrossRefGoogle ScholarPubMed
Evengård, B., Linder, E. & Lundbergh, P. (1988) Standardization of a filter-paper technique for blood sampling. Annals of Tropical Medicine and Parasitology, 82, 295–303.CrossRefGoogle ScholarPubMed
Giaever, I. (1976) Visual detection of carcinoembryonic antigen on surfaces. Journal of Immunology, 116, 766–771.CrossRefGoogle ScholarPubMed
Hancock, K. & Tsang, V. C. W. (1986) Development and optimization of the FAST-ELISA for detecting antibodies to Schistosoma mansoni. Journal of Immunological Methods, 92, 167–176.CrossRefGoogle ScholarPubMed
Idris, M. A. & Ruppel, A. (1988) Diagnostic 31/3200 Schistosoma mansoni proteins (Sm31/32): Reaction with sera from Sudanese patients infected with S. mansoni or S. haematobium. Journal of Helminthology, 62, 95–101.Google Scholar
Janitschke, K., Reinhold, A. & Bode, L. (1987) Nitrocellulose dot-ELISA for serodiagnosis of schistosomiasis. Transactions of the Royal Society of Tropical Medicine and Hygiene, 81, 956–958.CrossRefGoogle ScholarPubMed
Klinkert, M. O., Felleisen, R., Link, G., Ruppel, A. & Beck, E. (1989) Primary structures of Sm31/32 diagnostic proteins of Schistosoma mansoni and their identification as proteases. Molecular and Biochemical Parasitology, 33, 113–122.Google Scholar
Nilsson, L. Å., Björck, L. & Ouchterlouny, Ö. (1985) Paper discs impregnated with capillary blood. A sampling technique for immunoassays by means of DIG-ELISA and DIGTIA. Transactions of the Royal Society of Tropical Medicine and Hygiene, 79, 314–318.Google Scholar
Nilsson, L. Å. & Ouchterlouny, Ö. (1985) Aspects of simplified methods for serodiagnostic use in field work. Developments in Biological Standardization, 62, 11–16.Google Scholar
Ruppel, A., Breternitz, U. & Burger, R. (1987a) Diagnostic Mr 31.000 Schistosoma mansoni proteins: requirement of infection. but not immunization, and use of the “miniblot” technique for the production of monoclonal antibodies. Journal of Helminthology, 61, 95–101.CrossRefGoogle Scholar
Ruppel, A., Diesfeld, H. J. & Rother, U. (1985) Immunoblot analysis of Schistosoma mansoni antigens with sera of schistosomiasis patients: diagnostic potential of an adult schistosome polypeptide. Clinical and Experimental Immunology, 62, 499–506.Google Scholar
Ruppel, A., Idris, M. A., Sulaiman, S. M. & Hilali, A. M. H. (1990) Schistosoma mansoni diagnostic antigens (Sm 31/32): a seroepidemiological study in the Sudan. Tropical Medicine and Parasitology, 41, 127–130.Google ScholarPubMed
Ruppel, A., Shi, Y. E., Wei, D. X. & Diesfeld, H. J. (1987b) Sera of Schistosoma japonicum-infected patients cross-react with diagnostic 31/32 kD proteins of S. mansoni. Clinical and Experimental Immunology, 69, 291–298.Google Scholar
Shi, Y. E., Dell, R., Diesfeld, H. J., Burger, R., Wei, D. X. & Ruppel, A. (1988) Diagnostic 31/32 kD schistosome proteins in Schistosoma japonicum: reactivity in immunoblots with patient sera and monoclonal antibodies. Chinese Journal of Parasitology and Parasitic Diseases, 6, 245–248.Google Scholar