Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-10T12:36:54.475Z Has data issue: false hasContentIssue false
  • English
  • Français

Do aspermic (parthenogenetic) Fasciola forms have the ability to reproduce their progeny via parthenogenesis?

Published online by Cambridge University Press:  21 July 2021

Y. Ohari Open the ORCID record for Y. Ohari [Opens in a new window] ,
K. Hayashi ,
Y. Takashima  and
T. Itagaki Open the ORCID record for T. Itagaki [Opens in a new window]
Y. Ohari
Affiliation:
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka020-8550, Japan Department of Pathogenetic Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu501-1193, Japan
K. Hayashi
Affiliation:
Laboratory of Parasitology, Faculty of Veterinary Medicine, Okayama University of Science, 1-3 Ikoinooka, Imabari794-8555, Japan
Y. Takashima
Affiliation:
The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu501-1193, Japan Department of Veterinary Parasitology, Gifu University, Yanagido 1-1, Gifu501-1193, Japan
T. Itagaki*
Affiliation:
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka020-8550, Japan Department of Pathogenetic Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu501-1193, Japan
*
Author for correspondence: T. Itagaki, E-mail: itagaki@iwate-u.ac.jp
Get access Rights & Permissions [Opens in a new window]

Abstract

Across Far East Asia, aspermic Fasciola forms are found endemically. They have abnormal spermatogenesis and oogenesis, and it is presumed that their progeny are produced parthenogenetically and clonally. Because of this, they are also termed parthenogenic Fasciola forms. Currently, there is no evidence that they do indeed reproduce parthenogenetically and clonally. In this study, the multilocus genetic type (MLG) in 12 microsatellite markers of adult flukes and their subsequent progeny larvae were analysed using two laboratory aspermic Fasciola triploid strains. The MLGs of adults and their larvae were identical for all markers evaluated, suggesting that these strains reproduce their progeny clonally. The deviation between theoretical and actual frequency within the larvae genotype of the Fh_6 locus resulted in the inability for self-fertilization within individual adult flukes. These findings strongly suggested that aspermic Fasciola forms reproduce their progeny by means of parthenogenesis, possibly gynogenesis.

Keywords

Aspermic Fasciola formsmicrosatellite markerparthenogenesis
Type
Research Paper
Information
Journal of Helminthology , Volume 95 , 2021 , e36
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Cwiklinski, K, Allen, K, LaCourse, J, Williams, DJ, Paterson, S and Hodgkinson, JE (2015) Characterisation of a novel panel of polymorphic microsatellite loci for the liver fluke, Fasciola hepatica, using a next generation sequencing approach. Infection, Genetics and Evolution 32, 298–304.CrossRefGoogle ScholarPubMed
Dalton, JP (ed.) (1999) Fasciolosis. Wallingford, UK, CABI Pub, 544 pp.Google Scholar
Flanagan, SP and Jones, AG (2019) The future of parentage analysis: From microsatellites to SNPs and beyond. Molecular Ecology 28, 544–567.CrossRefGoogle ScholarPubMed
Hanna, REB, Moffett, D, Forster, FI, Trudgett, AG, Brennan, GP and Fairweather, I (2016) Fasciola hepatica: A light and electron microscope study of the ovary and of the development of oocytes within eggs in the uterus provides an insight into reproductive strategy. Veterinary Parasitology 221, 93–103.CrossRefGoogle ScholarPubMed
Hayashi, K, Ichikawa-Seki, M, Mohanta, UK, Shoriki, T, Chaichanasak, P and Itagaki, T (2018) Hybrid origin of Asian aspermic Fasciola flukes is confirmed by analyzing two single-copy genes, pepck and pold. Journal of Veterinary Medical Science 80, 98–102.CrossRefGoogle ScholarPubMed
Ichikawa, M and Itagaki, T (2010) Discrimination of the ITS1 types of Fasciola spp. Based on a PCR–RFLP method. Parasitology Research 106, 757–761.CrossRefGoogle ScholarPubMed
Itagaki, T, Kikawa, M, Sakaguchi, K, Shimo, J, Terasaki, K, Shibahara, T and Fukuda, K (2005) Genetic characterization of parthenogenic Fasciola sp. in Japan on the basis of the sequences of ribosomal and mitochondrial DNA. Parasitology 131, 679–685.CrossRefGoogle ScholarPubMed
Itagaki, T, Ichinomiya, M, Fukuda, K, Fusyuku, S and Carmona, C (2011) Hybridization experiments indicate incomplete reproductive isolating mechanism between Fasciola hepatica and Fasciola gigantica. Parasitology 138, 1278–1284. Cambridge University Press.CrossRefGoogle ScholarPubMed
Kamvar, ZN, Brooks, JC and Grünwald, NJ (2015) Novel R tools for analysis of genome-wide population genetic data with emphasis on clonality. Frontiers in Genetics 6, 208.CrossRefGoogle Scholar
Ohari, Y, Matsuo, K, Yoshida, A, Nonaka, N, Sato, H and Itagaki, T (2021) Genetic diversity and population structure analyses based on microsatellite DNA of parthenogenetic Fasciola flukes obtained from cattle and sika deer in Japan. Parasitology Research 120, 1341–1350.CrossRefGoogle ScholarPubMed
R Development Core Team (2020) R: A language and environment for statistical computing. Vienna, Austria, R Foundation for Statistical Computing.Google Scholar
Sakaguchi, Y (1980) Karyotype and gametogenesis of the common liver fluke. Fasciola sp. in Japan. Japanese Journal of Parasitology 29, 507–513.Google Scholar
Sasada, K (1978) Studies on the chromosomes of parasitic helminthes (II) triploidy and cytological mechanism of parthenogenesis in Diphyllobothrium erinacei (cestoda: Diphyllobothriidae). Japanese Journal of Parasitology 27, 547–560.Google Scholar
Shimizu, T and Yano, K (2011) A post-labeling method for multiplexed and multicolored genotyping analysis of SSR, indel and SNP markers in single tube with bar-coded split tag (BStag). BMC Research Notes 4, 161.CrossRefGoogle Scholar
Shoriki, T, Ichikawa-Seki, M, Suganuma, K, et al. (2016) Novel methods for the molecular discrimination of Fasciola spp. on the basis of nuclear protein-coding genes. Parasitology International 65, 180–183.CrossRefGoogle ScholarPubMed
Terasaki, K, Akahane, H, Habe, S and Moriyama, N (1982) The geographical distribution of common liver flukes (the genus Fasciola) with normal and abnormal spermatogenesis. The Japanese Journal of Veterinary Science 44, 223–231.CrossRefGoogle ScholarPubMed
Terasaki, K, Moriyama-Gonda, N and Noda, Y (1998) Abnormal spermatogenesis in the common liver fluke (Fasciola sp.) from Japan and Korea. Journal of Veterinary Medical Science 60, 1305–1309.CrossRefGoogle ScholarPubMed
Trouvé, S, Renaud, F, Durand, P and Jourdane, J (1996) Selfing and outcrossing in a parasitic hermaphrodite helminth (trematoda, echinostomatidae). Heredity 77, 1–8.CrossRefGoogle Scholar
Van Herwerden, L, Blair, D and Agatsuma, T (1999) Genetic diversity in parthenogenetic triploid Paragonimus westermani. International Journal for Parasitology 29, 1477–1482.CrossRefGoogle ScholarPubMed