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Dogs as alternative intermediate hosts of Taenia solium in Papua (Irian Jaya), Indonesia confirmed by highly specific ELISA and immunoblot using native and recombinant antigens and mitochondrial DNA analysis

Published online by Cambridge University Press:  12 April 2024

A. Ito ,
M.I. Putra ,
R. Subahar ,
M.O. Sato ,
M. Okamoto ,
Y. Sako ,
M. Nakao ,
H. Yamasaki ,
K. Nakaya  and
P.S. Craig
...Show all authors
A. Ito*
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan
M.I. Putra
Affiliation:
Animal Clinic of Hom-Hom, Veterinary Unit of Jayawijaya District, Irian Jaya, Indonesia
R. Subahar
Affiliation:
Department of Parasitology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
M.O. Sato
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan
M. Okamoto
Affiliation:
Department of Experimental Animal Sciences, Faculty of Veterinary Medicine, School of Agriculture, Tottori, Tottori University, Japan
Y. Sako
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan
M. Nakao
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan
H. Yamasaki
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan
K. Nakaya
Affiliation:
Animal Laboratory for Medical Research, Asahikawa Medical College, Asahikawa, Japan
P.S. Craig
Affiliation:
Cestode Zoonoses Research Group, Biosciences Research Institute, University of Salford, Salford, M5 4WT, UK
S.S. Margono
Affiliation:
Department of Parasitology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
*
*Fax: +81 166 68 2429 E-mail: akiraito@asahikawa-med.ac.jp
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Abstract

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Serology (ELISA and immunoblot) using native glycoproteins, affinity purified glycoproteins, and a recombinant antigen is known to be highly specific to Taenia solium cysticercosis in humans and pigs. These techniques were applied for dogs in the highly endemic area of cysticercosis in Papua (Irian Jaya), Indonesia. Analysis of dog sera by both ELISA and immunoblot revealed 7 of 64 dogs were highly positive. Examination of two sero-positive dogs revealed cysticerci of T. solium in the brain and heart of these dogs. Mitochondrial DNA analysis confirmed that they were the same as T. solium previously confirmed from pigs and biopsies from local people from Irian Jaya. It is suggested that the life cycle of T. solium may be completed not only between humans and pigs but also between humans and dogs.

Type
Research Article
Information
Journal of Helminthology , Volume 76 , Issue 4 , December 2002 , pp. 311 - 314
Copyright
Copyright © Cambridge University Press 2002

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