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The effect of levamisole on acetylcholinesterase secretion by male and female Heligmosomoides polygyrus during ageing

Published online by Cambridge University Press:  05 June 2009

S. Mallet  and
D. Kerboeuf
S. Mallet
Affiliation:
I.N.R.A. Centre de Recherches de Tours, Station de Pathologie Aviaire et de Parasitologie, Laboratoire d'Helminthologie, 37380 Nouzilly, France
D. Kerboeuf
Affiliation:
I.N.R.A. Centre de Recherches de Tours, Station de Pathologie Aviaire et de Parasitologie, Laboratoire d'Helminthologie, 37380 Nouzilly, France
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Abstract

The inhibition of acetylcholinesterase secretion by male and female Heligmosomoides polygyrus was tested on worms taken from experimentally infected mice and maintained for 3 days in vitro in levamisole. The dose inhibiting 50% of enzyme secretion (ID 50) of male worms was twice the ID 50 for female worms. A similar difference was observed in vivo between the dose of levamisole removing 50% (LD 50) of male and female worms from the mouse. Acetylcholinesterase secretion by worms and ID 50 were tested in vitro at 3-weekly intervals from 3 to 21 weeks post infection (WPI). Acetylcholinesterase secretion was always significantly higher for male than for female worms. A decrease of ID 50, correlated with the age of the worms was observed: from 1·5 to 0·5 μg/ml for males and from 0·7 to 0·1 μg/ml for females. These results were confirmed in vivo by a higher efficacy of the anthelminthic at 21 than at 4 WPI.

Keywords

Heligmosomoides polygyrusmouselevamisoleacetylcholinesteraseageingNematoda
Type
Research Papers
Information
Journal of Helminthology , Volume 67 , Issue 3 , September 1993 , pp. 243 - 247
Copyright
Copyright © Cambridge University Press 1993

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