Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-14T04:22:26.430Z Has data issue: false hasContentIssue false
  • English
  • Français

Encystment and excystment of the paramphistomid trematode Zygocotyle lunata

Published online by Cambridge University Press:  24 September 2010

K.E. LeSage  and
B. Fried
K.E. LeSage
Affiliation:
Department of Biology, Lafayette College, Easton, PA18042, USA
B. Fried*
Affiliation:
Department of Biology, Lafayette College, Easton, PA18042, USA
*
*E-mail: friedb@lafayette.edu
Get access Rights & Permissions [Opens in a new window]

Abstract

This study reports further observations on encystment and excystment of the paramphistomid trematode Zygocotyle lunata. Of numerous substrates tested in the laboratory for cercarial encystment, i.e. plastic and glass dishes, Styrofoam™, aluminium foil, snail shells, and cheesecloth, all but cheesecloth allowed for 100% encystment within 4 h of cercarial release at 28°C. Numerous physical and chemical factors were tested to kill cysts, and their capacity to do so was determined by the subsequent inability of cysts to excyst within a chemical excystment medium. Vinegar, Italian salad dressing, soy sauce and 10% solutions of sucrose, acetic acid and sodium chloride were the most detrimental. Freezing, boiling and storage of cysts for more than 2 years also killed them. Several marinades such as lemon juice, Worcestershire sauce, and various concentrations of potassium permanganate, did not kill the majority of cysts tested. Since Z. lunata is a potential foodborne trematode for humans and animals, it is important to determine suitable ways to control or kill these cysts.

Type
Research Papers
Information
Journal of Helminthology , Volume 85 , Issue 3 , September 2011 , pp. 300 - 303
Copyright
Copyright © Cambridge University Press 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ashrafi, K., Valero, M.A., Massoud, J., Sobhani, A., Solaymani-Mohammadi, S., Conde, P., Khoubbane, M., Bargues, M.D. & Mas-Coma, S. (2006) Plant-borne human contamination by fascioliasis. American Journal of Tropical Medicine and Hygiene 75, 295–302.CrossRefGoogle ScholarPubMed
Fried, B. & Wilson, B.D. (1981) Survival of metacercariae of Zygocotyle lunata (Trematoda) in half strength Locke's solution under refrigeration. Proceedings of the Helminthological Society of Washington 48, 96–97.Google Scholar
Fried, B., Huffman, J., Keeler, S. & Peoples, R.C. (2009) The biology of the caecal trematode Zygocotyle lunata. Advances in Parasitology 69, 1–40.CrossRefGoogle ScholarPubMed
Jones, A. (2005) Family Zygocotylidae. pp. 353–356in Jones, A., Bray, R.A. & Gibson, D.I. (Eds) Keys to the Trematoda, Vol. 2, London, UK, CABI.Google Scholar
Klockars, J., Huffman, J.E. & Fried, B. (2007) Survey of seasonal trematode infections in Helisoma trivolvis (Gastropoda) from lentic ecosystems in New, Jersey, U.S.A. Comparative Parasitology 74, 75–80.CrossRefGoogle Scholar
Ostrowski de Núñez, M., Spatz, L. & González Cappa, S.M. (2003) New intermediate hosts in the life cycle of Zygocotyle lunata in South America. Journal of Parasitology 89, 193–194.CrossRefGoogle ScholarPubMed
Peoples, R.C. & Fried, B. (2007) Effects of various physical and chemical factors on excystation of the encysted metacercariae of Echinostoma caproni. Journal of Parasitology 93, 1388–1391.Google Scholar
Peoples, R.C. & Fried, B. (2008) The effects of various chemical and physical factors on encystment and excystment of Zygocotyle lunata. Parasitology Research 108, 899–904.CrossRefGoogle Scholar
Saxton, T.M., Fried, B. & Peoples, R.C. (2008) Excystation of the encysted metacercariae of Echinostoma trivolvis and Echinostoma caproni in a trypsin–bile salts–cysteine medium and morphometric analysis of the excysted larvae. Journal of Parasitology 94, 669–671.CrossRefGoogle Scholar
Schmidt, K.A & Fried, B. (1997) Prevalence of larval trematodes in Helisoma trivolvis_ (Gastropoda) from a farm pond in Northampton County, Pennsylvania with special emphasis on Echinostoma trivolvis (Trematoda) cercariae. Journal of the Helminthological Society of Washington 64, 157–159.Google Scholar