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Fas2-ELISA in the detection of human infection by Fasciola hepatica

Published online by Cambridge University Press:  12 April 2024

J.R. Espinoza ,
O. Timoteo  and
P. Herrera-Velit
J.R. Espinoza*
Affiliation:
Molecular Biotechnology Unit, Laboratories for Research and Development, Faculty of Science and Philosophy, Universidad Peruana Cayetano Heredia, PO Box 4314, Lima, 100, Peru Department of Biology, Peruvian Institute of Nuclear Energy (IPEN), Peru
O. Timoteo
Affiliation:
Molecular Biotechnology Unit, Laboratories for Research and Development, Faculty of Science and Philosophy, Universidad Peruana Cayetano Heredia, PO Box 4314, Lima, 100, Peru
P. Herrera-Velit
Affiliation:
Molecular Biotechnology Unit, Laboratories for Research and Development, Faculty of Science and Philosophy, Universidad Peruana Cayetano Heredia, PO Box 4314, Lima, 100, Peru
*
*Fax: +511 319 0024, E-mail: jrespinoza@upch.edu.pe
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Abstract

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Fasciola hepatica has recently emerged as a major pathogen of humans from reports on areas of endemicity and hyper-endemicity for fascioliasis. This situation is aggravated by the lack of standard assays for the screen diagnosis of F. hepatica infection in humans living in endemic areas. Our laboratory has developed an enzyme-linked immunosorbent assay (Fas2-ELISA) based on the capture of IgG antibody by a purified protein Fas2, which is an adult fluke cysteine proteinase. Fas2-ELISA exhibited 95% sensitivity and 100% specificity in 38 individuals infected with F. hepatica diagnosed by finding eggs in stools and 46 serum samples from healthy volunteers. No cross-reaction was observed with 54 serum samples from patients with ten different parasitic infections including the trematodes Paragonimus westermani and Schistosoma mansoni. The high antigenicity of Fas2 is suggested by the fact that antibodies to Fas2 rise rapidly by 1–2 weeks of infection and rise until patency at 8 weeks of infection in experimentally infected alpacas. Field screening for human fascioliasis using Fas2-ELISA and coprology in three endemic locations of the Peruvian Andes resulted in 95.5% sensitivity, 86.6% specificity in a population of 664 children in an age range of 1 to 16 years old. These results provide evidence of the clinical potential of Fas2-ELISA to diagnose fascioliasis in humans exposed to liver fluke infection in endemic areas for this parasite. Fas2-ELISA is currently developed as a standard assay for both field screening for fascioliasis in people living in endemic areas and detecting occasionally F. hepatica infected patients in clinical laboratories.

Type
Review Article
Information
Journal of Helminthology , Volume 79 , Issue 3 , September 2005 , pp. 235 - 240
Copyright
Copyright © Cambridge University Press 2005

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