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Genetic variation in the triploids of Japanese Fasciola species, and relationships with other species in the genus

Published online by Cambridge University Press:  05 June 2009

T. Agatsuma ,
K. Terasaki ,
L. Yang  and
D. Blair
T. Agatsuma
Affiliation:
Department of Parasitology, Kochi Medical School, Nankoku City, Kochi 783, Japan
K. Terasaki
Affiliation:
Laboratory of Biology, St Mary's Junior College, Tsubuku-Honmachi, Kurume City, Fukuoka 830, Japan
L. Yang
Affiliation:
Department of Medical Chemistry, Kochi Medical School, Nankoku City, Kochi 783, Japan
D. Blair
Affiliation:
Department of Zoology, James Cook University, Townsville, Australia
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Abstract

Twelve enzymes (encoded by 14 loci) in liver flukes of Fasciola species originating from Japan (parthenogenetic triploids), Korea (parthenogenetic diploids), the United States of America (USA) and Australia (all sexual diploids) were analysed using starch gel electrophoresis. Variation in electrophoretic patterns between samples was detected at five enzyme loci (Ak, Got, Gpi, 6-Pgd and Pgm-2). Japanese worms (31, of which six were established as uniparental laboratory strains), which reproduce by parthenogenesis, exhibited three different isozyme patterns. This indicates that triploidy has arisen more than once in Japanese flukes. Japanese Fasciola sp. can be separated into three types on morphological grounds. For the six laboratory strains of Japanese worms, the parental morphological type was known. Each of the three isozyme patterns observed was restricted to one morphological type. Most alleles detected in the Japanese triploids were also found in diploid worms from the other countries: the only alleles not represented elsewhere were four at the Got locus and two at the Pgm locus. Flukes from a laboratory strain derived from a single Korean diploid worm resembled the Japanese worms in genotype more closely than did American (seven uniparental laboratory strains) or Australian (30 worms) specimens. Worms from the last two countries were closely related.

Type
Research Papers
Information
Journal of Helminthology , Volume 68 , Issue 3 , September 1994 , pp. 181 - 186
Copyright
Copyright © Cambridge University Press 1994

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References

Agatsuma, T. (1981) Electrophoretic studies on enzymes in the Japanese common liver fluke, Fasciola sp. II. On further three enzymes, GPI, CDH and ME, in the liver fluke. Japanese Journal of Parasitology 30, 157–159.Google Scholar
Agatsuma, T. & Habe, S. (1985) Electrophoretic studies on enzymes of diploid and triploid Paragonimus westermani. Parasitology 91, 489–497.CrossRefGoogle ScholarPubMed
Agatsuma, T. & Habe, S. (1986) Genetic variability and differentiation of natural populations in three Japanese lung flukes, Paragonimus ohirai, P. iloktsuenensis and P. sadoensis (Digenea: Troglotrematidae). Journal of Parasitology 2, 201–208.Google Scholar
Agatsuma, T. & Suzuki, N. (1980) Electrophoretic studies on enzymes in the Japanese common liver fluke, Fasciola sp. Japanese Journal of Medical Science and Biology 33, 249–254.CrossRefGoogle ScholarPubMed
Blair, D. & McManus, D.P. (1989) Restriction enzyme mapping of ribosomal DNA can distinguish between fascioloid (liver fluke) species. Molecular and Biochemical Parasitology 36, 201–208.CrossRefGoogle ScholarPubMed
Itagaki, H. & Akane, S. (1959) Morphological study on the Japanese liver fluke, compared with the African specimens. Bulletin of the Azabu Veterinary College 6, 115–123.Google Scholar
Moriyama, N., Tsuji, M. & Seto, T. (1979) Three karyotypes and their phenotypes of Japanese liver flukes (Fasciola sp.). Japanese Journal of Parasitology 28, 23–33.Google Scholar
Oshima, T., Akahane, H. & Shimazu, T. (1969) Patterns of the variation of the common liver flukes (Fasciola sp.) in Japan. I. Variations in the sizes and shapes of the worms and eggs. Japanese Journal of Parasitology 17, 97–105.Google Scholar
Sakaguchi, Y. (1980) Karyotype and gametogenesis of the common liver fluke, Fasciola sp., in Japan. Japanese Journal of Parasitology 17, 97–105.Google Scholar
Sakaguchi, Y. & Nakagawa, C. (1975) A note on the chromosomes of the common liver flukes (Fasciola sp.) from Japan. Chromosome Information Service 19, 20–21.Google Scholar
Sakaguchi, Y. & Ueno, H. (1977) Studies on the chromosome of helminths (6). Chromosomes of a liver fluke, Fasciola hepatica from Australia. Japanese Journal of Parasitology 26, (Suppl.), 67.Google Scholar
Terasaki, K. (1977) Studies on chromosomes of the lung flukes in Japan. Japanese Journal of Parasitology 26, 222–229.Google Scholar
Terasaki, K., Akahane, H., Habe, S. & Moriyama, N. (1982) The geographical distribution of common liver flukes (the genus Fasciola) with normal and abnormal spermatogenesis. Japanese Journal of Veterinary Science 44, 223–231.Google ScholarPubMed
Ueno, H. & Watanabe, S. (1960) Ecological studies on the common liver fluke in Japan. Kachiku Eisei Shikenjo Hokoku 38. 167–181 (in Japanese).Google Scholar