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Heligmosomoides polygyrus: effect of exogenous steroid hormones on egg output in vitro

Published online by Cambridge University Press:  05 June 2009

Tammi L. Richardson  and
Barbara M. Mackinnon
Tammi L. Richardson
Affiliation:
Department of Biology, University of New Brunswick, Fredericton, New Brunswick, Canada E3B 6E1
Barbara M. Mackinnon
Affiliation:
Department of Biology, University of New Brunswick, Fredericton, New Brunswick, Canada E3B 6E1
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Abstract

The effect of exogenous steroid hormones on the egg output of Heligmosomoides polygyrus (Nematoda: Trichostrongylidae) was examined in vitro. Using worms raised in female mice, it was found that estradiol, testosterone, and cortisone each significantly decreased egg output. Although similar trends were found using H. polygyrus raised in male mice, none of the decreases found was significant. No significant differences were found with ecdysone or progesterone treatments using worms from female or male mice. Treatment of worms with cortisone did not significantly affect retention of eggs within the uterus of H. polygyrus. Titration of the effect of cortisone on egg output indicated that levels of reduction were significant for concentrations of 5·6 × 10−6 M to 5·6 × 10−3 M in worms from female mice and for concentrations of 5·6 × 10−8, × 10−7, × 10−5 and × 10−3 in worms from male mice. Radioisotope labelling experiments showed incorporation of 3H-corticosterone in the nucleus of intestinal cells of H. polygyrus suggesting that its effect on egg production may be via a modulatory effect on the intestinal cells.

Keywords

Heligmosomoides polygyrusmouseegg outputsteroidsautoradiography
Type
Research Article
Information
Journal of Helminthology , Volume 64 , Issue 2 , June 1990 , pp. 123 - 132
Copyright
Copyright © Cambridge University Press 1990

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