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A hidden break in the 28.0S rRNA from Diphyllobothrium dendriticum

Published online by Cambridge University Press:  05 June 2009

K. A. Karlstedt ,
G. I. L. Paatero ,
J.-H. Mäkelä  and
B.-J. Wikgren
K. A. Karlstedt
Affiliation:
Department of Biology, Åbo Akademi University, SF-20520 Åbo, Finland
G. I. L. Paatero
Affiliation:
Department of Biology, Åbo Akademi University, SF-20520 Åbo, Finland
J.-H. Mäkelä
Affiliation:
Department of Biology, Åbo Akademi University, SF-20520 Åbo, Finland
B.-J. Wikgren
Affiliation:
Department of Biology, Åbo Akademi University, SF-20520 Åbo, Finland
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Abstract

Nondenatured and denatured total RNA from the tapeworm Diphyllobothrium dendriticum (Cestoda) was analysed by agarose gel electrophoresis. It was found that the large subunit ribosomal RNA (lrRNA) is 28.0S and the small subunit ribosomal RNA (srRNA) is 19.5S. Following denaturation the 28.0S rRNA was disrupted into a 19.5S subfragment and a 20.7S subfragment due to the presence of a centrally located hidden break. By hybridization of Northern blot membranes with oligonucleotide probes specific for the 5′- and 3′-ends of the lrRNA respectively, we have shown that the 19.5S sub-fragment is from the 5′-end (the α-subfragment) and the 20.7S subfragment from the 3′-end (the β-subfragment) of the 28.0S rRNA of D. dendriticum.

Keywords

Diphyllobothrium dendriticumrRNAhidden breakNorthern blotoligonucleotide probesCestoda
Type
Research Article
Information
Journal of Helminthology , Volume 66 , Issue 3 , September 1992 , pp. 193 - 197
Copyright
Copyright © Cambridge University Press 1992

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