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In vitro stress selection of nematophagous fungi for biocontrol of parasitic nematodes in ruminants

Published online by Cambridge University Press:  05 June 2009

M. Larsen ,
J. Wolstrup ,
S. A. Henriksen ,
C. Dackman ,
J. Grønvold  and
P. Nansen
M. Larsen
Affiliation:
Department of Veterinary Microbiology, Animal Biotechnology Research Center, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C., Denmark
J. Wolstrup
Affiliation:
Department of Ecology and Molecular Biology, Section of Microbiology, Royal Veterinary and Agricultural University, 21 Rolighedsvej, DK-1870 Frederiksberg C., Denmark
S. A. Henriksen
Affiliation:
National Veterinary Laboratory, 27 Bülowsvej, DK-1870 Frederiksberg C., Denmark
C. Dackman
Affiliation:
Department of Veterinary Microbiology, Animal Biotechnology Research Center, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C., Denmark
J. Grønvold
Affiliation:
Laboratory of Parasitology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C., Denmark
P. Nansen
Affiliation:
Laboratory of Parasitology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C., Denmark
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Abstract

Laboratory experiments were designed to select nematophagous fungi that were able to survive in vitro conditions simulating passage through the gastro-intestinal tract of cattle. All of the tests were conducted at 39°C. In a primary stress selection step in diluted rumen fluid, 21 isolates were obtained. Each of the primary stress selected isolates was tested in synthetic saliva, rumen fluid simulating the activity in the rumen, rumen fluid followed by pepsin-hydrochloric acid treatment simulating the additional effect of ruminal and abomasal activity, pepsin-hydrochloric acid solution simulating conditions in the abomasum and finally in a trypsin solution as an example of enzyme activity in the gut. The effect of the rumen fluid alone, or rumen fluid followed by pepsin-hydrochloric acid treatment, were responsible for the reduction in surviving fungal isolates. Only six of thirteen isolates belonging to the genus Arthrobotrys survived while seven of eight isolates of the genus Duddingtonia survived. Fourteen isolates were tested for their predatory capacity in a dung pat bioassay. Fungi of the genera Arthrobotrys and Duddingtonia reduced the development of Ostertagia ostertagi third stage larvae by approximately 75% and 96% respectively compared to the number of larvae that developed from fungus-free control pats.

Keywords

nematophagous fungiArthrobotrysDuddingtoniastress selectionOstertagia ostertagibiological control
Type
Research Papers
Information
Journal of Helminthology , Volume 65 , Issue 3 , September 1991 , pp. 193 - 200
Copyright
Copyright © Cambridge University Press 1991

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References

REFERENCES

Barron, G. L. (1977) The nematode-destroying fungi. Topics in Mycobiology No. 1, Canadian Biological Publications Ltd., Canada.Google Scholar
Cooke, R. C. & Godfrey, B. E. S. (1964) A key to the nematode-destroying fungi. A key to the nematode-destroying fungi, 47, 61–74.Google Scholar
Dackman, C., Olsson, S., Jansson, H. -B., Lundgren, B. & Nordbring-Hertz, B. (1987) Quantification of predatory and endoparasitic nematophagous fungi in soil. Quantification of predatory and endoparasitic nematophagous fungi in soil, 3, 89–93.Google Scholar
Domsch, K. H., Gams, W. & Anderson, T. -H. (1980) Arthrobotrys Corda 1839. In: Compendium of Soil Fungi, vol. 1 pp. 59–64, Academic Press.Google Scholar
Grønvold, J., Wolstrup, J., Henriksen, S. A. & Nansen, P. (1987) Field experiments on the ability of Arthrobotrys oligospora (Hyphomycetales) to reduce the number of larvae of Cooperia oncophora (Trichostrongylidae) in cow pats and surrounding grass. Field experiments on the ability of Arthrobotrys oligospora (Hyphomycetales) to reduce the number of larvae of Cooperia oncophora (Trichostrongylidae) in cow pats and surrounding grass, 61, 65–71.Google ScholarPubMed
Grønvold, J., Nansen, P., Henriksen, S. A., Thylin, J. & Wolstrup, J. (1988) The capability of the predacious fungus Arthrobotrys oligospora (Hyphomycetales) to reduce numbers of infective larvae of Ostertagia ostertagi (Trichostrongylidae) in cow pats and herbage during the grazing season in Denmark. Journal of Helminthology, 62, 271–280.CrossRefGoogle ScholarPubMed
Grønvold, J., Henriksen, S. A., Nansen, P., Wolstrup, J. & Thylin, J. (1989) Attempts to control infection with Ostertagia ostertagi (Trichostrongylidae) in grazing calves by adding mycelium of the nematode-trapping fungus Arthrobotrys oligospora (Hyphomycetales) to cow pats. Attempts to control infection with Ostertagia ostertagi (Trichostrongylidae) in grazing calves by adding mycelium of the nematode-trapping fungus Arthrobotrys oligospora (Hyphomycetales) to cow pats, 63, 115–126.Google Scholar
Haard, K. (1968) Taxonomic studies on the genus Arthrobotrys Corda. Taxonomic studies on the genus Arthrobotrys Corda, 60, 1140–1159.Google Scholar
Hvelplund, T. & Wolstrup, J. (1974) Growth of cellulolytic bacteria in the in-vitro fermentation tube. Den kgl. Veteriœrog Landbohøjskole. Årsskrift 1974, 101–107.Google Scholar
Jarowaja, N. (1970) Rodzaj Arthrobotrys Corda (The genus Arthrobotrys Corda). Rodzaj Arthrobotrys Corda (The genus Arthrobotrys Corda), 6, 337–406.Google Scholar
Mcdougall, E. I. (1948) Studies on ruminant saliva. 1. The composition and output of sheep's saliva. Biochemical Journal 43, 99–109.CrossRefGoogle ScholarPubMed
Nordbring-Hertz, B. (1972) Scanning electron microscopy of the nematode-trapping fungus Arthrobotrys oligospora. Scanning electron microscopy of the nematode-trapping fungus Arthrobotrys oligospora, 26, 279–284.Google Scholar
Nordbring-Hertz, B. (1977) Nematode-induced morphogenesis in the predacious fungus Arthrobotrys oligospora. Nematologica, 23, 443–451.CrossRefGoogle Scholar
Van Oorschot, C. A. N. (1985) Taxonomy of the Dactylaria complex, V. A review of Arthrobotrys and allied genera. Studies in Mycology, 26, 61–96.Google Scholar
Schenck, S., Kendrick, W. B., & Pramer, D. (1977) A new nematode-trapping hyphomycete and a re-evaluation of Dactylaria and Arthrobotrys. A new nematode-trapping hyphomycete and a re-evaluation of Dactylaria and Arthrobotrys, 55, 977–985.Google Scholar
Tilley, J. M. A. & Terry, R. A. (1963) A two-stage technique for the in vitro digestion of forage crops. A two-stage technique for the in vitro digestion of forage crops, 18, 104–111.Google Scholar