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In vivo selection for Haemonchus contortus resistance to monepantel

Published online by Cambridge University Press:  18 March 2019

S.C.M. Niciura Open the ORCID record for S.C.M. Niciura [Opens in a new window] ,
G.G. Cruvinel Open the ORCID record for G.G. Cruvinel [Opens in a new window] ,
C.V. Moraes Open the ORCID record for C.V. Moraes [Opens in a new window] ,
A.C.S. Chagas Open the ORCID record for A.C.S. Chagas [Opens in a new window] ,
S.N. Esteves Open the ORCID record for S.N. Esteves [Opens in a new window] ,
M.V. Benavides Open the ORCID record for M.V. Benavides [Opens in a new window]  and
A.F.T. Amarante Open the ORCID record for A.F.T. Amarante [Opens in a new window]
S.C.M. Niciura*
Affiliation:
Embrapa Pecuária Sudeste, Rodovia Washington Luiz, Km 234, Fazenda Canchim, CEP 13560- 970, São Carlos, SP, Brazil
G.G. Cruvinel
Affiliation:
Centro Universitário Central Paulista, Rua Miguel Petroni, 5111, CEP 13563-470, São Carlos, SP, Brazil
C.V. Moraes
Affiliation:
Universidade Federal de São Carlos, Rodovia Washington Luiz, Km 235, CEP 13566-905, São Carlos, SP, Brazil
A.C.S. Chagas
Affiliation:
Embrapa Pecuária Sudeste, Rodovia Washington Luiz, Km 234, Fazenda Canchim, CEP 13560- 970, São Carlos, SP, Brazil
S.N. Esteves
Affiliation:
Embrapa Pecuária Sudeste, Rodovia Washington Luiz, Km 234, Fazenda Canchim, CEP 13560- 970, São Carlos, SP, Brazil
M.V. Benavides
Affiliation:
Embrapa Pecuária Sul, Rodovia BR-153, Km 632,9, Vila Industrial, CEP 96401-970, Bagé, RS, Brazil
A.F.T. Amarante
Affiliation:
UNESP, Instituto de Biociências, Rua Professor Doutor Antônio Celso Wagner Zanin, 250, Distrito de Rubião Junior, CEP 18618-689, Botucatu, SP, Brazil
*
Author for correspondence: S.C.M. Niciura, E-mail: simone.niciura@embrapa.br
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Abstract

Gastrointestinal nematodes significantly affect the ovine industry, and Haemonchus contortus is considered the most pathogenic parasite in tropical regions. This situation is aggravated when the main strategy to control worms fails because of the genetic resistance that parasites acquire against anthelmintics. Aiming to anticipate the events involved in anthelmintic resistance, we induced monepantel resistance in H. contortus by in vivo subdosing of sheep hosts. Four successive passages of a monepantel-susceptible H. contortus isolate in Santa Ines or Ile de France sheep hosts resulted in three monepantel-resistant (efficacy varying from 0 to 58.5%) H. contortus isolates. Sheep hosts were treated from 0.075 mg/kg to the therapeutic dose of 2.5 mg/kg of monepantel in 19–26 rounds of selection for 112–133 weeks. Success in inducing H. contortus resistance to monepantel may have been affected by worm burden and by host–parasite interactions, including a possible effect of the breed of sheep hosts. We conclude that subdosing of sheep, although time-consuming, is an efficient in vivo strategy for the induction of monepantel resistance in H. contortus. The resistant parasites can be used in further studies to elucidate the genetic and biochemical events involved in the acquisition of anthelmintic resistance.

Keywords

Induction of anthelmintic resistancegastrointestinal nematodeslow-dose selectionhost-parasite interaction
Type
Short Communication
Information
Journal of Helminthology , Volume 94 , 2020 , e46
Copyright
Copyright © Cambridge University Press 2019 

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