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Isolation, purification and characterization of surface antigens of the bovine filarial parasite Setaria digitata for the immunodiagnosis of bancroftian filariasis

Published online by Cambridge University Press:  05 June 2009

J. Gerald Theodore  and
P. Kaliraj
J. Gerald Theodore
Affiliation:
Center for Biotechnology, Anna University, Madras 600 025, India
P. Kaliraj
Affiliation:
Center for Biotechnology, Anna University, Madras 600 025, India
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Abstract

The surface antigens of the bovine filarial parasite Setaria digitata were isolated by EDTA extraction and purified by affinity chromatography using sepharose bound human filarial (Wuchereria bancrofti) antibodies obtained from chronic human filarial sera. The purified and crude antigens were used in enzyme-linked immunosorbent assay (ELISA) for the detection of serum antibodies in bancroftian filariasis. The purified antigen showed sensitive and specific reactions in ELISA for the detection of antibodies in filarial sera and showed least cross reactivity with other parasitic infections. The crude and purified antigens showed about 18 and 6 peptide bands respectively in SDS-PAGE and about 11 and 6 antigenic bands respectively in enzyme-linked immunoelectrotransfer blot (EITB). The purified antigen was observed to be glycoprotein in nature. It was possible to identify the stage-specific infection in human filariasis by using the crude and purified antigens in EITB.

Keywords

Setaria digitatasurface antigensimmunodiagnosisELISAWuchereria bancrofti
Type
Research Article
Information
Journal of Helminthology , Volume 64 , Issue 2 , June 1990 , pp. 105 - 114
Copyright
Copyright © Cambridge University Press 1990

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References

REFERENCES

Baschong, W., Tanner, M., Betschart, B., Rudin, W. & Weiss, N. (1982) Dipetalonema viteae: Extraction and immunogenicity of antigens from female worms. Experimental Parasitology, 53, 262–269.CrossRefGoogle ScholarPubMed
Cabrera, Z. & Parkhouse, R. M. E. (1986) Identification of antigens of Onchocerca volvulus and Onchocerca gibsoni for diagnostic use. Molecular and Biochemical Parasitology, 20, 225.CrossRefGoogle ScholarPubMed
Cuatrecasas, P. & Anfinsen, C. B. (1971) Enzyme purification and related techniques. In: Affinity chromatography—Methods in Enzymology, (editor William, B. J.) Vol. 22, pp. 345–378, Academic Piess: New York.Google Scholar
Dasgupta, A. & Bala, S. (1978) Litmosoides curinii— soluble antigen in circulation and immunosuppression in vitro. Indian Journal of Medical Research, 67, 30–33.Google Scholar
Dissanayake, S. & Ismail, M. M. (1980) Antigens of Setaria digitata: Cross reaction with surface antigens of Wuchereria bancrofti microfilariae and serum antibodies in W. bancrofti infected subjects. Bulletin of the World Health Organization, 58, 649–654.Google ScholarPubMed
Forsyth, K. P., Copeman, D. B., Anders, R. F. & Mitchell, G. F. (1981) The major radioiodinated cuticular antigens of Onchocerca gibsoni microfilariae are neither species nor Onchocerca specific. Acta Tropica, 38, 343–352.Google ScholarPubMed
Harinath, B. C. (1984) lmmunodiagnosis of bancroftian filariasis—problems and progress. Journal of Bioscience, 6, 691.CrossRefGoogle Scholar
Kaliraj, P., Ghirnikar, S. N. & Harinath, B. C. (1981a) Immunodiagnosis of Bancroftian filariasis: Comparative efficiency of the indirect haemagglutination test, indirect fluorescent antibody test and enzyme linked immunosorbent assay done with W. bancrofti mf antigen. American Journal of Tropical Medicine and Hygiene, 30, 982–987.CrossRefGoogle Scholar
Kaliraj, P., Ghirnikar, S. N. & Harinath, B. C. (1981b) Enzyme linked immunosorbent assay for Bancroftian filariasis. Transactions of the Royal Society of Tropical Medicine and Hygiene, 75, 119–120.CrossRefGoogle ScholarPubMed
Kaliraj, P., Kharat, I., Ghirnikar, S. N. & Harinath, B. C (1981c) The utility of human filarial serum in the detection of circulating antigen. Journal of Helminthology, 55, 133–139.CrossRefGoogle ScholarPubMed
Kaliraj, P., Patel, M. T., Desai, G., Megha, R. & Desai, P. K. (1983) Utility of human anti-hepatitis B surface antigen serum in purification and detection of hepatitis B surface antigen. Indian Journal of Experimentel Biology, 21, 487–493.Google ScholarPubMed
Kaushal, N. A., Hussain, R. & Ottesen, E. A. (1984) Excretory-secretory and somatic antigens in the diagnosis of human filariasis. Clinical and Experimental Immunology, 56, 567.Google ScholarPubMed
Kent, N. H. (1963) Comparative immunochemistry of farval and adult worms of Schistosoma mansoni. Annals of the New York Academy of Sciences, 133, 100.CrossRefGoogle Scholar
Laemmli, U. K. (1970) Cleavage of structural protein during the assembly of the head of the bacteriophage T4. Nature, 227, 680.CrossRefGoogle ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. I. & Randall, R. J. (1951) Protein measurement with the Folin-Phenol reagent. Journal of Biological Chemistry, 193, 265.CrossRefGoogle ScholarPubMed
Maizels, R. M., Partona, F., Oemijati, S. & Ogilvie, B. M. (1983) Antigenic analysis of Brugia timori, a filarial nematode of man: Initial characterization by surface radioiodination and evaluation of diagnostic potential. Clinical and Experimental Immunology, 51, 269–277.Google Scholar
Mok, W. Y., Buckley, H. R. & Campbell, C. C. (1977) Characterization of antigens from type A and B yeast cells of Histoplasma capsulation. Infection and Immunity, 16, 461–466.CrossRefGoogle Scholar
Nash, T. E., Nasir, U. D. & Jeanloz, R. W (1977) Further purification and characterization of a circulation antigen in schistosomiasis. Journal of Immunology, 119, 1627–1643.CrossRefGoogle ScholarPubMed
Ottesen, E. A. (1984) Immunological aspects of lymphatic filariasis and onchocerciasis in man. Transactions of the Royal Society of Tropical Medicine and Hygiene, 78 (Suppl), 9–17.CrossRefGoogle ScholarPubMed
Philipp, M., Gomez-Priego, A., Parkhouse, R. M. E., Davies, M. W., Clark, N. W. T., Ogilvie, B. M. & Beltran-Hernandez, F (1984) Identification of an antigen of Onchocerca volvulus of possible diagnostic use. Parasitology, 89, 295.CrossRefGoogle ScholarPubMed
Reddy, M. V. R., Malhotra, A. & Harinath, B. C. (1984) Detection of circulating antigen in bancroftian filariasis by sandwich ELISA using filarial serum IgG. Journal of Helminthology, 58, 259.CrossRefGoogle ScholarPubMed
Rolfe, R. D. & Finegold, S. M. (1979) Purification and characterization of Clostridium difficile toxin. Infection and Immunity, 25, 191–201.CrossRefGoogle ScholarPubMed
Ruitenberg, E. J., Steerenberg, P. A. & Brosi, B. J. M. (1975) Microsystem for the application of ELISA in the serodiagnosis of Trichinella spiralis. Infections Medikon Nederland, 4, 30–31.Google Scholar
Sawada, T., Sato, K. & Sato, S. (1969) Studies on the skin test antigen FST for immunodiagnosis of filariasis. Electrophoretic analysis and fractionation of antigen, FST. Japanese Journal of Experimental Medicine, 39, 427–433.Google ScholarPubMed
Sharma, S. P., Biswas, H., Das, M. & Dwivedi, S. R. (1983) Present status of the filariasis problem in India. Journal of Communicable Diseases, 15, 53.Google ScholarPubMed
Tanner, C. E. (1983) Immunochemical study of the antigens of Trichinella spiralis II. Some physiochemical properties of these antigens. Experimental Parasitology, 14, 337–345.CrossRefGoogle Scholar
Towbin, H., Staehelin, T. & Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Procedure and some applications. Proceedings of the National Academy of Sciences, USA, 76, 4350.CrossRefGoogle ScholarPubMed
Tsang, V. C. W., Peralta, J. M. & Simons, A. R. (1983) The enzyme-linked immuno electro-transfer blot techniques (EITB) for studying the specificity of antigens and antibodies separated by gel electrophoresis. Methods in Enzymology, 92, 377.CrossRefGoogle Scholar