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Meriones unguiculatus infected by Haemonchus contortus: evaluation of different experimental protocols

Published online by Cambridge University Press:  19 April 2018

L.T. Gressler ,
T.H. Grando ,
G. Machado do Carmo ,
R.A. Vaucher ,
L.M. Stefani  and
S.G. Monteiro
L.T. Gressler*
Affiliation:
Laboratory of Veterinary Parasitology, Universidade Federal de Santa Maria (UFSM), Avenida Roraima 1000, 97105-900 Santa Maria, RS, Brazil
T.H. Grando
Affiliation:
Laboratory of Veterinary Parasitology, Universidade Federal de Santa Maria (UFSM), Avenida Roraima 1000, 97105-900 Santa Maria, RS, Brazil
G. Machado do Carmo
Affiliation:
Laboratory of Veterinary Parasitology, Universidade Federal de Santa Maria (UFSM), Avenida Roraima 1000, 97105-900 Santa Maria, RS, Brazil
R.A. Vaucher
Affiliation:
Postgraduate Program on Biochemistry and Bioprospecting, Center for Chemistry, Pharmaceutical and Food Science, Universidade Federal de Pelotas (UFPel), Rua dos Ipês 1206, 96010-900 Pelotas, RS, Brazil
L.M. Stefani
Affiliation:
Postgraduate Program on Animal Science, Universidade do Estado de Santa Catarina (UDESC), Rua Beloni Trombeta Zanin 680E, 89815-630 Chapecó, SC, Brazil
S.G. Monteiro*
Affiliation:
Laboratory of Veterinary Parasitology, Universidade Federal de Santa Maria (UFSM), Avenida Roraima 1000, 97105-900 Santa Maria, RS, Brazil
*
Authors for correspondence: L.T. Gressler, E-mail: ltgressler@gmail.com S.G. Monteiro, E-mail: sgmonteiro@uol.com.br
Authors for correspondence: L.T. Gressler, E-mail: ltgressler@gmail.com S.G. Monteiro, E-mail: sgmonteiro@uol.com.br
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Abstract

Many important studies on resistance reversion, anthelmintic efficacy and, especially, new molecules with antiparasitic effects are performed in laboratories using gerbils (Meriones unguiculatus) as the experimental model. This study aimed to evaluate the use of corticosteroids (dexamethasone and methylprednisolone acetate) in gerbils experimentally infected with different doses of infective larvae (sheathed or exsheathed) of Haemonchus contortus. In the first experiment, 28 gerbils were divided into seven groups infected by 2–6 × 103 larvae, with or without immunosuppression using corticosteroids. In the second experiment, eight gerbils were divided into two groups infected by 2 × 103 sheathed or exsheathed larvae. For the third assay, seven immunosuppressed gerbils were infected with 2 × 103 sheathed larvae and were killed 15 days post infection (PI). The highest number of parasites was recovered from methylprednisolone-immunosuppressed animals. We observed red and white blood cell alterations and biochemical parameters in infected animals that had undergone immunosuppression with methylprednisolone. We highlight that in the first and second experiments a satisfactory number of worms was recovered using sheathed larvae and immunocompetent animals. When exsheathed larvae were used, the number of worms recovered was unsatisfactory. A considerable larval burden was recovered from immunosuppressed gerbils 15 days PI, and body weight did not influence establishment of larvae.

Type
Research Paper
Information
Journal of Helminthology , Volume 93 , Issue 4 , July 2019 , pp. 447 - 452
Copyright
Copyright © Cambridge University Press 2018 

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