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Molecular and morphological characterization of the tapeworm Taenia hydatigena (Pallas, 1766) in sheep from Iran

Published online by Cambridge University Press:  08 October 2013

S. Rostami ,
R. Salavati ,
R.N. Beech ,
Z. Babaei ,
M. Sharbatkhori ,
M.R. Baneshi ,
E. Hajialilo ,
H. Shad  and
M.F. Harandi
S. Rostami
Affiliation:
Department of Medical Parasitology, School of Medicine, Kerman University of Medical Sciences, Kerman76169-14111, Iran
R. Salavati
Affiliation:
Institute of Parasitology, McGill University, Ste. Anne de Bellevue, Quebec, Canada
R.N. Beech
Affiliation:
Institute of Parasitology, McGill University, Ste. Anne de Bellevue, Quebec, Canada
Z. Babaei
Affiliation:
Department of Medical Parasitology, School of Medicine, Kerman University of Medical Sciences, Kerman76169-14111, Iran
M. Sharbatkhori
Affiliation:
Department of Medical Parasitology and Mycology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran
M.R. Baneshi
Affiliation:
Research Center for Modeling in Health, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran
E. Hajialilo
Affiliation:
Department of Medical Parasitology and Mycology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
H. Shad
Affiliation:
Iranian Veterinary Organization, Shahr-e-Ray, Tehran, Iran
M.F. Harandi*
Affiliation:
Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman76169-14111, Iran
*
*Fax: +98-341-3221676 E-mail: fasihi@kmu.ac.ir
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Abstract

Although Taenia hydatigena is one of the most prevalent taeniid species of livestock, very little molecular genetic information exists for this parasite. Up to 100 sheep isolates of T. hydatigena were collected from 19 abattoirs located in the provinces of Tehran, Alborz and Kerman. A calibrated microscope was used to measure the larval rostellar hook lengths. Following DNA extraction, fragments of cytochrome c oxidase 1 (CO1) and 12S rRNA genes were amplified by the polymerase chain reaction method and the amplicons were subjected to sequencing. The mean total length of large and small hooks was 203.4 μm and 135.9 μm, respectively. Forty CO1 and 39 12S rRNA sequence haplotypes were obtained in the study. The levels of pairwise nucleotide variation between individual haplotypes of CO1 and 12S rRNA genes were determined to be between 0.3–3.4% and 0.2–2.1%, respectively. The overall nucleotide variation among all the CO1 haplotypes was 9.7%, and for all the 12S rRNA haplotypes it was 10.1%. A significant difference was observed between rostellar hook morphometry and both CO1 and 12S rRNA sequence variability. A significantly high level of genetic variation was observed in the present study. The results showed that the 12S rRNA gene is more variable than CO1.

Type
Research Papers
Information
Journal of Helminthology , Volume 89 , Issue 2 , March 2015 , pp. 150 - 157
Copyright
Copyright © Cambridge University Press 2013 

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