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Monoamine oxidase in amphistomes and its role in worm motility

Published online by Cambridge University Press:  12 April 2024

S.M.A. Abidi  and
W.A. Nizami
S.M.A. Abidi*
Affiliation:
Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh-202 002, India
W.A. Nizami
Affiliation:
Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh-202 002, India
*
*Fax: +91 571 408069 E-mail: zyr015@amu.up.nic.in
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Abstract

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The quantitative assay of mitochondrial monoamine oxidase (MAO) activity revealed a higher enzyme level in Explanatum explanatum than Gastrothylax crumenifer. The specific MAO inhibitors, chlorgyline, pargyline, deprenyl and nialamide produced different degrees of interspecific inhibition. The differential effects on enzyme activity of chlorgyline and deprenyl suggests the possible existence of polymorphic forms of the enzyme, MAO-A and MAO-B, in amphistomes. These specific inhibitors also had a differential influence on the in vitro motility of amphistomes, further indicating the involvement of different forms of MAO in the oxidative deamination of biogenic monoamines which might be partly responsible for neuromuscular coordination in amphistomes. The experimental procedures used in this study could be conveniently used for quick screening and evaluation of some of the qualitative effects of anthelmintic drugs under in vitro conditions.

Type
Research Article
Information
Journal of Helminthology , Volume 74 , Issue 4 , December 2000 , pp. 283 - 288
Copyright
Copyright © Cambridge University Press 2000

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