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Morphological and genetic variation of Wuchereria bancrofti microfilariae in carriers in Thailand, Lao PDR and Myanmar: evaluation using Giemsa-stained thick blood films

Published online by Cambridge University Press:  30 September 2019

J. Jongthawin ,
P.M. Intapan ,
T. Thanchomnang ,
L. Sadaow ,
S. Laymanivong ,
W. Maleewong Open the ORCID record for W. Maleewong [Opens in a new window]  and
O. Sanpool Open the ORCID record for O. Sanpool [Opens in a new window]
J. Jongthawin
Affiliation:
Faculty of Medicine, Mahasarakham University, Maha Sarakham 44000, Thailand Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
P.M. Intapan
Affiliation:
Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
T. Thanchomnang
Affiliation:
Faculty of Medicine, Mahasarakham University, Maha Sarakham 44000, Thailand Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
L. Sadaow
Affiliation:
Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
S. Laymanivong
Affiliation:
Centre of Malariology, Parasitology and Entomology, Ministry of Health, Vientiane, Lao PDR
W. Maleewong
Affiliation:
Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
O. Sanpool*
Affiliation:
Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
*
Author for correspondence: O. Sanpool, E-mail: sanpoolor@yahoo.com, oransa@kku.ac.th
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Abstract

There is geographical variation in the morphology and genetics of Wuchereria bancrofti, the major cause of human lymphatic filariasis. This study aims to compare morphological and genetic variation of W. bancrofti microfilariae recovered from carriers in Lao PDR, Myanmar and Thailand. Six morphological parameters (body length, cephalic space length and width, length of head to nerve ring, body width at nerve ring, Innenkȍrper length and number of column nuclei between the cephalic space and nerve ring) were evaluated from microfilariae in Giemsa-stained thick blood films. A portion of the cytochrome c oxidase subunit 1 gene of mitochondrial DNA was sequenced and analysed. Wuchereria bancrofti microfilariae showed a wide variation in their morphology and morphometry among three countries. Phylogenetic analysis confirmed that all microfilariae belonged to W. bancrofti. Higher mutation frequencies were observed in samples from Myanmar, relative to Thailand and Lao PDR. This study highlights the morphological disparities of microfilariae and genetic variability within W. bancrofti among three geographical locations. We found that reported morphometric differences between localities were less clear-cut than previously thought. Further studies are needed to determine the microfilarial periodicity in Lao PDR.

Keywords

cox-1 genegenetic variationlymphatic filariasismicrofilariamorphologyWuchereria bancrofti
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e95
Copyright
Copyright © Cambridge University Press 2019 

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