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Morphological and molecular characterization of an enigmatic clinostomid trematode (Digenea: Clinostomidae) parasitic as metacercariae in the body cavity of freshwater fishes (Cichlidae) across Middle America

Published online by Cambridge University Press:  17 May 2018

R. Briosio-Aguilar ,
M. García-Varela ,
D.I Hernández-Mena ,
M. Rubio-Godoy  and
G. Pérez-Ponce de León
R. Briosio-Aguilar
Affiliation:
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México (UNAM), Avenida Universidad 3000, Ciudad Universitaria, CP 04510, Distrito Federal, México Posgrado en Ciencias Biológicas, UNAM
M. García-Varela
Affiliation:
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México (UNAM), Avenida Universidad 3000, Ciudad Universitaria, CP 04510, Distrito Federal, México
D.I Hernández-Mena
Affiliation:
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México (UNAM), Avenida Universidad 3000, Ciudad Universitaria, CP 04510, Distrito Federal, México
M. Rubio-Godoy
Affiliation:
Instituto de Ecología, A.C. Red de Biología Evolutiva, km 2.5 Ant. Carretera a Coatepec, Xalapa, 91070 Veracruz, Mexico
G. Pérez-Ponce de León*
Affiliation:
Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México (UNAM), Avenida Universidad 3000, Ciudad Universitaria, CP 04510, Distrito Federal, México
*
Author for correspondence: G. Pérez-Ponce de León E-mail: ppdleon@ib.unam.mx
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Abstract

The family Clinostomidae Lühe, 1901 contains 29 species allocated to seven genera, of which Clinostomum Leidy, 1856 is the most diverse, with c. 14 valid species. The diversity of Clinostomum has been assessed, combining morphological and molecular data. The genetic library for species in this genus has increased steadily, although there is little or no information for the other genera included in the family. Molecular phylogenetic relationships among the genera of clinostomids have not been assessed, and their classification is still based on morphological traits. The monotypic Ithyoclinostomum was described from a fish-eating bird in Brazil, and its metacercariae have been found in several locations in South America, parasitizing erythrinid freshwater fishes. We collected unusually large metacercariae from the body cavity of cichlids in several locations across Middle America. These metacercariae exhibited some resemblance to Ithyoclinostomum, although several differences prevent their inclusion in Ithyoclinostomum dimorphum, casting doubt on their taxonomic identification. The main objective of this paper was to characterize the metacercariae collected in cichlids using both morphology and molecular data from three molecular markers, and to assess the molecular phylogenetic relationships among the genera of Clinostomidae to establish the position of the newly generated sequences. We took a conservative position and tentatively placed the metacercariae as belonging to Ithyoclinostomum.

Type
Research Paper
Information
Journal of Helminthology , Volume 93 , Issue 4 , July 2019 , pp. 461 - 474
Copyright
Copyright © Cambridge University Press 2018 

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