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Morphology and molecular analysis of Paratylenchus nanjingensis n. sp. (Nematoda: Paratylenchinae) from the rhizosphere soil of Pinus massoniana in China

Published online by Cambridge University Press:  23 December 2014

K. Wang ,
H. Xie ,
Y. Li ,
W.J. Wu  and
C.L. Xu
K. Wang
Affiliation:
Laboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Guangdong Province Key Laboratory of Microbial Signals and Disease Control/Department of Plant Pathology, College of Nature Resources and Environment, South China Agricultural University, Guangzhou510642, China
H. Xie*
Affiliation:
Laboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Guangdong Province Key Laboratory of Microbial Signals and Disease Control/Department of Plant Pathology, College of Nature Resources and Environment, South China Agricultural University, Guangzhou510642, China
Y. Li
Affiliation:
Laboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Guangdong Province Key Laboratory of Microbial Signals and Disease Control/Department of Plant Pathology, College of Nature Resources and Environment, South China Agricultural University, Guangzhou510642, China
W.J. Wu
Affiliation:
Laboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Guangdong Province Key Laboratory of Microbial Signals and Disease Control/Department of Plant Pathology, College of Nature Resources and Environment, South China Agricultural University, Guangzhou510642, China
C.L. Xu
Affiliation:
Laboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Guangdong Province Key Laboratory of Microbial Signals and Disease Control/Department of Plant Pathology, College of Nature Resources and Environment, South China Agricultural University, Guangzhou510642, China
*
*E-mail: xiehui@scau.edu.cn
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Abstract

Paratylenchus nanjingensis n. sp. was obtained from Nanjing, Jiangsu Province, China. This new species is characterized by having a female with a slender, vermiform body (243–279 μm), head with distinct submedian lobes, slender and long stylet (64–68 μm), anchor-shaped stylet knobs, excretory pore anterior to the level of the stylet knobs, small lateral vulval flaps and lateral field with four lines; and male with more distinct body annuli, stylet lacking and pharynx degenerate. The internal transcribed spacer sequences of ribosomal RNA (ITS rRNA) gene of the new species were amplified and sequenced in this study. The phylogenetic relationships of the new species with other Paratylenchus species using the ITS rRNA gene sequences are given.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 2 , March 2016 , pp. 166 - 173
Copyright
Copyright © Cambridge University Press 2014 

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References

Allen, M.W. & Sher, S.A. (1967) Taxonomic problems concerning the phytoparasitic nematodes. Annual Review of Phytopathology 5, 247–264.Google Scholar
Andrássy, I. (2007) Free-living nematodes of Hungary, II (Nematoda errantia). 496 pp. Budapest, Hungarian Natural History Museum.Google Scholar
Braun, A.L. & Lownsbery, B.F. (1975) The pin nematode, Paratylenchus neoamblycephalus, on myrobalan plum and other hosts. Journal of Nematology 7, 336–343.Google Scholar
Brzeski, M.W. (1963) Paratylenchus macrodorus n. sp. (Nematoda: Paratylenchidae), a new plant parasitic nematode from Poland. Bulletin de I'Académie Polonaise des Sciences, Classe II 11, 277–280.Google Scholar
Brzeski, M.W. (1995) Paratylenchinae: morphology of some known species and descriptions of Gracilacus bilineata sp. n. and G. vera sp. n. (Nematoda: Tylenchulidae). Nematologica 41, 535–565.Google Scholar
Brzeski, M.W. & Háněl, L. (2000) Paratylenchinae: evaluation of diagnostic morpho-biometrical characters of females in the genus Paratylenchus Micoletzky, 1922 (Nematoda: Tylenchulidae). Nematology 2, 253–261.Google Scholar
Chen, D.Y., Ni, H.F., Tsay, T.T. & Yen, J.H. (2008) Identification of Gracilacus bilineata and G. aculenta (Nematoda: Criconematoidea, Tylenchulidae) among bamboo plantations in Taiwan. Plant Pathology Bulletin 17, 209–219.Google Scholar
De Luca, F., Reyes, A., Troccoli, A. & Castillo, P. (2011) Molecular variability and phylogenetic relationships among different species and populations of Pratylenchus (Nematoda: Pratylenchidae) as inferred from the analysis of the ITS rDNA. European Journal of Plant Pathology 130, 415–426.Google Scholar
Esser, R.P. (1992) A diagnostic compendium to species included in Paratylenchinae Thorne, 1949 and Tylenchocriconematinae Raski & Siddiqui, 1975 (Nematoda: Criconematoidea). Nematologica 38, 146–163.Google Scholar
Geraert, E. (1965) The genus Paratylenchus . Nematologica 11, 301–334.Google Scholar
Ghaderi, R., Kashi, L. & Karegar, A. (2014) Contribution to the study of the genus Paratylenchus Micoletzky, 1922 sensu lato (Nematoda: Tylenchulidae). Zootaxa 3841, 151–187.Google Scholar
Golden, A.M. (1961) Paratylenchus steineri (Criconematidae) a new species of plant nematode. Proceedings of the Helminthological Society of Washington 28, 9–11.Google Scholar
Golden, A.M. (1971) Classification of the genera and higher categories of the Order Tylenchida (Nematoda). pp. 191–232 in Zukerman, B.M., Mai, W.F. & Rohde, R.A. (Eds) Plant parasitic nematodes. New York, Academic Press.Google Scholar
Hu, M. & Gasser, R.B. (2006) Mitochondrial genomes of parasitic nematodes – progress and perspectives. Trends in Parasitology 22, 78–84.CrossRefGoogle Scholar
Huang, C.S. & Raski, D.J. (1986) Four new species of Gracilacus Raski, 1962 (Criconematoidea: Nemata). Revue de Nématologie 9, 347–356.Google Scholar
Huelsenbeck, J.P. & Ronquist, F. (2001) Mrbayes: Bayesian inference of phylogenetic trees. Bioinformatics 17, 754–755.Google Scholar
Jenkins, W.R. (1960) Paratylenchus marylandicus n. sp. (Nematoda: Criconematidae) associated with roots of pine. Nematologica 5, 175–177.Google Scholar
Kashi, L., Karegar, A. & Kheiri, A. (2009) Paratylenchus paraperaticus sp. n. (Tylenchida: Tylenchulidae) found in the rhizosphere of walnut trees in Hamadan province, Iran. Nematology 11, 641–647.Google Scholar
Liu, W.Z. (2004) Description of the species of plant parasitic nematodes. 666 pp. Beijing, China Agriculture Press.Google Scholar
Pereira, T.L., Santos, U., Schaefer, C.E., Souza, G.O., Paiva, S.R., Malabarba, L.R., Schmidt, E.E. & Dergam, J.A. (2013) Dispersal and vicariance of Hoplias malabaricus (Bloch, 1794) (Teleostei, Erythrinidae) populations of the Brazilian continental margin. Journal of Biogeography 40, 905–914.Google Scholar
Powers, T.O., Harris, T., Higgins, R., Sutton, L. & Powers, K.S. (2010) Morphological and molecular characterization of Discocriconemella inarata, an endemic nematode from North American native tallgrass prairies. Journal of Nematology 42, 35–45.Google Scholar
Pramodini, M., Mohilal, N. & Dhanachand, C. (2006) Gracilacus vitecus sp. n. and record of G. raskii Phukan & Sanwal from Manipur, India. Indian Journal of Nematology 36, 272–276.Google Scholar
Raski, D.J. (1962) Paratylenchidae n. fam. with descriptions of five new species of Gracilacus n.g. and an emendation of Cacopaurus Thorne, 1943, Paratylenchus Micoletzky, 1922 and Criconematidae Thorne, 1943. Proceedings of the Helminthological Society 29, 189–207.Google Scholar
Raski, D.J. (1976) Revision of the genus Paratylenchus Micoletzky, 1922 and descriptions of new species. Part III of three parts – Gracilacus . Journal of Nematology 8, 97–115.Google Scholar
Raski, D.J. (1991) Tylenchulidae in agricultural soils. pp. 761–794 in Nickle, W.R. (Ed.) Manual of agricultural nematology. New York, Marcel Dekker.Google Scholar
Seinhorst, J.M. (1959) A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica 4, 67–69.Google Scholar
Siddiqi, M.R. (2000) Tylenchida: Parasites of plants and insects. 2nd edn. 833 pp. Wallingford, UK, CABI Publishing.Google Scholar
Siddiqi, M.R. & Goodey, J.B. (1964) The status of the genera and subfamilies of the Criconematidae (Nematoda); with a comment on the position of Fergusobia . Nematologica 9, 363–377.Google Scholar
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28, 2731–2739.Google Scholar
Thorne, G. & Malek, R.B. (1968) Nematodes of the Northern Great Plains. Part I. Tylenchida (Nemata: Secernentea). 111 pp. South Dakota, Agricultural Experiment Station, South Dakota University.Google Scholar
Van den Berg, E. & Cadet, P. (1991) One new and some known plant parasitic nematode species from the French Caribbean (Nemata: Tylenchina). Revue de Nématologie 14, 389–405.Google Scholar
Van den Berg, E., Tiedt, L.R. & Subbotin, S.A. (2014) Morphological and molecular characterisation of several Paratylenchus Micoletzky, 1922 (Tylenchida: Paratylenchidae) species from South Africa and USA, together with some taxonomic notes. Nematology 16, 323–358.Google Scholar
Vrain, T.C., Wakarchuk, D.A., Levesque, A.C. & Hamilton, R.I. (1992) Intraspecific rDNA restriction fragment length polymorphism in the Xiphinema americanum group. Fundamental and Applied Nematology 15, 563–573.Google Scholar
Wang, J.L., Zhang, J.C. & Gu, J.F. (2011) Method of extracting DNA from a single nematode. Plant Quarantine 25, 32–35.Google Scholar
Wang, K., Xie, H., Li, Y., Xu, C.L., Yu, L. & Wang, D.W. (2013) Paratylenchus shenzhenensis n. sp. (Nematoda: Paratylenchinae) from the rhizosphere soil of Anthurium andraeanum in China. Zootaxa 3750, 167–175.Google Scholar
Xie, H. (2005) Taxonomy of plant nematodes. 2nd edn. 435 pp. Beijing, China, Higher Education Press.Google Scholar