Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-14T04:33:14.990Z Has data issue: false hasContentIssue false
  • English
  • Français

The pattern of peripheral blood leucocyte changes in mice infected with Nematospiroides dubius

Published online by Cambridge University Press:  18 November 2009

Najah M. H. Ali ,
Jerzy M. Behnke  and
B. R. Manger
Najah M. H. Ali
Affiliation:
Experimental Parasltology Unit, Department of Zoology, University of Nottingham, University Park, Nottingham NG7 2RD, UK
Jerzy M. Behnke
Affiliation:
Beecham Pharmaceuticals, Animal Health Research Centre, Walton Oakes, Dorking Road, Tadworth, Surrey KT20 7NT, UK
B. R. Manger
Affiliation:
Beecham Pharmaceuticals, Animal Health Research Centre, Walton Oakes, Dorking Road, Tadworth, Surrey KT20 7NT, UK
Get access Rights & Permissions [Opens in a new window]

Extract

Experiments were carried out to define the haematological changes taking place during the first six weeks of a primary infection with Nematospiroides dubius. The general pattern of changes was observed to comprise a rapid increase in circulating leucocytes (4 to 5-fold increase) which consisted of a neutropl a, lymphocytosis, monocytosis and an eosinophilia. However, in strong responder NIH mice leucocyte counts returned to normal more rapidly than in other strains (by day 28). In contrast, in weak responder C57BL/10 mice the leucocyte counts whilst falling significantly relative to day 7 did not return to normal within the experimental period. Mice infected with irradiated larvae did not experience as high a leucocytosis as did mice given an identical number of normal larvae. The peak lymphocytosis, neutrophilia and monocytosis were all lower. The removal of adult worms from infected animals by treatment with pyrantel on days 9,11,13 and 16, also significantly altered the pattern of leucocytosis. The neutrophilia which was evident on day 7 returned rapidly to normal, whereas in mice which had retained their worms a peak neutrophilia was observed on day 14. These haematological changes were discussed and related to the failure of hostprotective immunity to operate effectively during the early stages of a primary infection with N. dubius.

Type
Research Papers
Information
Journal of Helminthology , Volume 59 , Issue 1 , March 1985 , pp. 83 - 93
Copyright
Copyright © Cambridge University Press 1985

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Ali, N. M. H. & Behnke, J. M. (1983) Nematospiroides dubius: factors affecting the primary response to SRBC in infected mice. Journal of Helminthology, 57, 343–353.CrossRefGoogle ScholarPubMed
Ali, N. M. H. & Behnke, J. M. (1984) Non–specific immunodepression by Nematospiroides dubius of concurrent responses to oxazolone and lipopolysaccharide. Journal of Helminthology, 58,301–311.Google Scholar
Ali, N. M. H. & Behnke, J. M. (1985) Observations on the gross changes in the secondary lymphoid organs of mice infected with Nematospiroides dubius. Journal of Helminthology (in press).Google ScholarPubMed
Baker, N. F. (1962) The nature and etiology of the leukocytic response of Webster mice infected with Nematospiroides dubius. Journal of Parasitology, 48, 438–441.CrossRefGoogle ScholarPubMed
Behnke, J. M., Hannah, J. & Pritchard, D. I. (1983) Nematospiroides dubius in the mouse: evidence that adult worms depress the expression of homologous immunity. Parasite Immunology, 5, 397–408.CrossRefGoogle ScholarPubMed
Behnke, J. M. & Parish, H. A. (1979) Nematospiroides dubius: arrested development of larvae in immune mice. Experimental Parasitology, 47, 116–127.CrossRefGoogle ScholarPubMed
Behnke, J. M., Parish, H. A. & Hagan, P. (1980) The effect of gamma irradiation on Nematospiroides dubius. Factors affecting the survival of worms in a primary infection in mice. Journal of Helminthology, 54, 173–182.CrossRefGoogle ScholarPubMed
Behnke, J. M. & Wakelin, D. (1977) Nematospiroides dubius: stimulation of acquired immunity in inbred strains of mice. Journal of Helminthology, 51, 167–176.CrossRefGoogle ScholarPubMed
Boyer, M. H., Spry, C. J. F., Beeson, P. B. & Sheldon, W. H. (1971) Mechanism of cosinophilia. IV. The pulmonary lesion resulting from intravenous injection of Trichinclla spiralis. Yale Journal of Biology and Medicine, 43, 351–356.Google Scholar
Cayzer, C. J. R. & Dobson, C (1983) Suppression of antibody production in mice given multiple concurrent infections with Nematospiroides dubius. International Journal for Parasitology, 13, 61–65.CrossRefGoogle ScholarPubMed
Cypess, R. H. (1972) Blood cell count changes associated with immunity to Nematospiroides dubius. Journal of Parasitology, 58, 563–566.CrossRefGoogle ScholarPubMed
Ehrenford, F. A. (1954) The life cycle of Nematospiroides dubius Baylis (Nematoda: Heligmosomidae). Journal of Parasitology, 40, 480–481.CrossRefGoogle Scholar
Hagan, P., Behnke, J. M. & Parish, H. A. (1981) Stimulation of immunity to Nematospiroides dubius in mice using larvae attenuated by cobalt 60 irradiation. Parasite Immunology, 3, 149–156.CrossRefGoogle ScholarPubMed
Hurley, J. C. & Vadas, M. A. (1983) Eosinophilia and acquisition of resistance to Nematospiroides dubius in mice sensitized with adult worms. Australian Journal of Experimental Biology and Medical Science, 61, 1–9.CrossRefGoogle ScholarPubMed
Jenkins, S. N. & Behnke, J. M. (1977) Impairment of primary expulsion of Trichuris muris in mice concurrently infected with Nematospiroides dubius. Parasitiology, 75, 71–78.CrossRefGoogle ScholarPubMed
Jones, C. E. & Rubin, R. (1974) Nematospiroides dubius: mechanisms of host immunity. I. Parasite counts, histopathology and scrum transfer involving orally or subcutancously sensitized mice. Expertmental Parasitology, 35, 434–452.CrossRefGoogle ScholarPubMed
Liu, S. K. (1965) Pathology of Nematospiroides dubius. I. Primary infections in C3H and Webster mice. Experimental Parasitology, 17, 123–135.CrossRefGoogle ScholarPubMed
Moqbel, R. (1980) Histopathological changes following primary, secondary and repeated infections of rats with Strongyloides ratti, with special reference to tissue cosinophils. Parasite Immunology, 2, 11–27.CrossRefGoogle Scholar
Ogilvie, B. M., Askenase, P. W. & Rose, M. E. (1980) Basophils and eosinophils in three strains of rats and in athymic(nude) rats following infection with the nematodes Nippostrongylus brasiliensis or Trichinella spiralis. Immunology, 39, 385–389.Google ScholarPubMed
Ogilvie, B. M., Hesketh, P. M. & Rose, M. E. (1978) Nippostrongylus brasiliensis: Peripheral blood leucocyte response of rats, with special reference to basophils. Experimental Parasitology, 46, 20–30.CrossRefGoogle ScholarPubMed
Pritchard, D. I., Williams, D. J. L., Behnke, J. M. & Lee, T. D. G. (1983) The role of lgG1 hypergammaglobulinaemia in immunity to the gastrointestinal nematode Nematospiroides dubius. The immunochemical purification, antigen specificity and in vivo antiparasite effect of lgG1 from immune serum. Immunology, 49, 353–365.Google Scholar
Prowse, S. J., Ey, P. L. & Jenkin, C. R. (1978) Immunity to Nematospiroides dubius: cell and immunoglobulin changes associated with the onset of immunity in mice. Australian Journal of Experimental Biology and Medicine, 56, 237–246.CrossRefGoogle ScholarPubMed
Przyjalkowski, Z., Kontny, E. & Cabaj, W. (1980) Haematological changes evoked by Trichinella spiralis as an index of the course to trichincllosis in germ free and conventional mice. Bulletin de L'Académie Polonaise des Sciences, 28, 87–93.Google ScholarPubMed
Ramalho-Pinto, F. J., Rossi, R. & Smithers, S. R. (1979) Murine schistosomiasis mansoni: antischistosomula complement and cosinophil-mediated killing of schistosomula in vitro. Parasite Immunology, 1, 295–308.CrossRefGoogle ScholarPubMed
Roth, R. L. & Levy, D. A. (1980) Nippostrongylus brasiliensis: Peripheral leukocyte responses and correlation of basophils with blood histamine concentration during infection in rats. Experimental Parasitology, 50, 331–341.CrossRefGoogle ScholarPubMed
Ruitenberg, E. J., Elgersma, A., Kruizinga, W. & Leenstra, F. (1977) Trichinella spiralis infection in congcnitally athymic (nude) mice. Parasitological, scrological and hacmatological studies with observations on intestinal pathology. Immunology, 33, 581–587.Google ScholarPubMed
Sokal, R. R. & Rohlf, F. J. (1969) Biometry. Freeman, San Francisco.Google Scholar
Wakelin, D. & Donachie, A. M. (1983) Genetic control of cosinophilia. Mouse strain variation in response to antigens of parasite origin. Clinical and Experimental Immunology, 51, 239–246.Google Scholar
Williams, D. J. & Behnke, J. M. (1983) Host protective antibodies and scrum immunoglobulin isotypes in mice chronically infected or repeatedly immunized with the nematode parasite Nematospiroides dubius. Immunology, 48, 37–47.Google ScholarPubMed
Yarinsky, A. (1962) The influence of X-irradiation on the immunity of mice to infection with Trichinella spiralis. Journal of the Elisha Mitchell Scientific Society, 78, 29–43.Google Scholar