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Purification and partial characterization of proteinase activity in eggs of Dicrocoelium dendriticum

Published online by Cambridge University Press:  12 April 2024

C. Giménez-Pardo ,
C. de Armas-Serra  and
F. Rodríguez-Caabeiro
C. Giménez-Pardo*
Affiliation:
Parasitology Laboratory, Faculty of Pharmacy, Alcalá University, Crtra. Madrid–Barcelona Km 33.600, 28871 Alcalá de Henares (Madrid), Spain
C. de Armas-Serra
Affiliation:
Parasitology Laboratory, Faculty of Pharmacy, Alcalá University, Crtra. Madrid–Barcelona Km 33.600, 28871 Alcalá de Henares (Madrid), Spain
F. Rodríguez-Caabeiro
Affiliation:
Parasitology Laboratory, Faculty of Pharmacy, Alcalá University, Crtra. Madrid–Barcelona Km 33.600, 28871 Alcalá de Henares (Madrid), Spain
*
*Fax: +34 1 885 46 63 E-mail: consuelo.gimenez@uah.es
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Abstract

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A preliminary purification has been carried out by continuous elution electrophoresis of a 49.5 kDa protease of crude extracts from Dicrocoelium dendriticum eggs. The enzyme showed a high capacity to degrade the collagen derivative azocoll at acidic pH. Although it is necessary to carry out further experiments to confirm any physiological role, this protease could be implicated in penetration mechanisms.

Type
Research Article
Information
Journal of Helminthology , Volume 75 , Issue 1 , January 2001 , pp. 57 - 59
Copyright
Copyright © Cambridge University Press 2001

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