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Role of T cell subpopulations in mice infected with Angiostrongylus cantonensis

Published online by Cambridge University Press:  05 June 2009

J.D. Lee ,
J.J. Wang ,
J.H. Chang ,
L.Y. Chung ,
E.R. Chen  and
C.M. Yen
J.D. Lee
Affiliation:
Department of Parasitology, Kaohsiung Medical College, Kaohsiung, Taiwan, Republic of China
J.J. Wang
Affiliation:
Department of Parasitology, Kaohsiung Medical College, Kaohsiung, Taiwan, Republic of China
J.H. Chang
Affiliation:
Department of Parasitology, Medical College, Cheng Kung University, Tainan, Taiwan, Republic of China
L.Y. Chung
Affiliation:
Department of Parasitology, Kaohsiung Medical College, Kaohsiung, Taiwan, Republic of China
E.R. Chen
Affiliation:
Department of Parasitology, Kaohsiung Medical College, Kaohsiung, Taiwan, Republic of China
C.M. Yen*
Affiliation:
Department of Parasitology, Kaohsiung Medical College, Kaohsiung, Taiwan, Republic of China
*
* Author for correspondence.
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Abstract

When C57BL/6 mice were infected with Angiostrongylus cantonensis, the percentage of T helper (CD4+) cells and T supressor (CD8+) cells in peripheral blood increased weekly until the third and seventh week respectively, and then gradually decreased. C57BL/6 mice were depleted of CD4+ and CD8+ T cells by in vivo injection of anti-CD4 and anti-CD8 monoclonal antibodies, respectively, and then infected with A. cantonensis. There were significantly more and less worms recovered in the mice depleted of CD4+ and CD8+ T cells respectively than in undepleted mice. Discrete subpopulations of T cells from mice exposed to A. cantonensis for 3 weeks or 7 weeks were adoptively transferred to syngeneic recipients which were then given a challenge infection. Protection was mediated by a CD4+ T cell population present in mice after 3 weeks of infection but was not demonstrable with cells taken 7 weeks after infection. When CD4+ T cells obtained from 3-week infected mice were mixed with 5% CD8+ T cells obtained from mice infected for 7 weeks, no significant transfer of resistance was observed. Thus, immune responses to A. cantonensis in mice were regulated by discrete subpopulations of T lymphocytes.

Type
Research Papers
Information
Journal of Helminthology , Volume 70 , Issue 3 , September 1996 , pp. 211 - 214
Copyright
Copyright © Cambridge University Press 1996

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References

Bugarski, D., Sofronic-Milosavljevic, L.J. & Movsesijan, M. (1992) Susceptibility and the immune response to Trichinella spiralis infection. Glas-Srpska Akademija Nauka i Umetnosti, Odeljenje Medicinskih Nauka 42, 83–91.Google Scholar
Feldmeiri, G., Katz, N., Rocha, R.S., Abrantes, W., DaSilva, A.L. & Ginzinelli, G. (1983) Immune responses during human schistosomiasis mansoni. IX. T-lymphocyte subset analysis by monoclonal antibodies in hepatosplenic disease. Scandinavian Journal of Immunology 17, 297–302.Google Scholar
Grencis, R.K., Riedlinger, J. & Wakelin, D. (1985) L3T4-positive T lymphoblasts are responsible for transfer of immunity to Trichinella spiralis in mice. Immunology 56, 213–218.Google ScholarPubMed
Miltenyi, S., Muller, W., Weichel, W. & Radbruch, A. (1990) High gradient magnetic cell separation with MACS. Cytometry 11, 231–238.CrossRefGoogle ScholarPubMed
Perez, O., Capron, M., Lastre, M., Venge, P., Khalife, J. & Capron, A. (1989) Angiostrongylus cantonensis: Role of eosinophils in the neurotoxic syndrome (Gordon-like phenomenon). Experimental Parasitology 68, 403–413.CrossRefGoogle ScholarPubMed
Phillips, S.M., Walker, D., Abdel-Hafez, S.K., Linette, G.P., Doughty, B.L., Perrin, P.J. & Fathelbab, N. (1987) The immune response to Schistosoma mansoni infections in inbred rats. VI. Regulation by cell subpopulations. Journal of Immunology 139, 2781–2787.CrossRefGoogle ScholarPubMed
Sasaki, O., Sugaya, H., Ishida, K. & Yoshimura, K. (1993) Ablation of eosinophils with anti-IL-5 antibody enhances the survival of intracranial worms of Angiostrongylus cantonensis in the mouse. Parasite Immunology 15, 349–354.CrossRefGoogle ScholarPubMed
Sugaya, H. & Yoshimura, K. (1988) T-cell-dependent eosinophilia in the cerebrospinal fluid of the mouse infected with Angiostrongylus cantonensis. Parasite Immunology 10, 127–138.CrossRefGoogle ScholarPubMed
Wang, J.J. (1993) Comparison of Thl- and Th2-associated immune reactivities of mice infected with Angiostrongylus cantonensis. Master thesis, Institute of Medicine, Kaohsiung Medical College, Taiwan, Republic of China.Google Scholar
Yen, C.M. & Chen, E.R. (1991) Detection of antibodies to Angiostrongylus cantonensis in serum and cerebrospinal fluid of patients with eosinophilic meningitis.International Journal for Parasitology 21, 17–21.Google ScholarPubMed
Yen, C.M., Chen, E.R., Hsieh, H.C. & Dow, W.Y. (1987) Angiostrongylus cantonensis infection in immunosuppressed mice. Japanese Journal of Parasitology 36, 397–404.Google Scholar
Yoshimura, K. (1989) The eosinophil in parasitic infections, pp. 7–46in Ko, R.C. (Ed.) Current concepts in parasitology. Hong Kong, Hong Kong University Press.Google Scholar
Yoshimura, K., Sato, K., Uchida, K. & Oya, H. (1982) The course of Angiostrongylus cantonensis infection in athymic nude and neonatally thymectomized mice. Zeitschrift für Parasitenkunde 67, 217–226.CrossRefGoogle ScholarPubMed