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Screening for Indian isolates of egg-parasitic fungi for use in biological control of fascioliasis and amphistomiasis in ruminant livestock

Published online by Cambridge University Press:  01 September 2008

S. De ,
P.K. Sanyal ,
A.K. Sarkar ,
N.K. Patel ,
S. Pal  and
S.C. Mandal
S. De
Affiliation:
Department of Parasitology, College of Veterinary Science and Animal Husbandry, Indira Gandhi Krishi Viswavidyalaya, Anjora, Durg-491 001, Chhattisgarh, India
P.K. Sanyal*
Affiliation:
Department of Parasitology, College of Veterinary Science and Animal Husbandry, Indira Gandhi Krishi Viswavidyalaya, Anjora, Durg-491 001, Chhattisgarh, India
A.K. Sarkar
Affiliation:
Department of Parasitology, College of Veterinary Science and Animal Husbandry, Indira Gandhi Krishi Viswavidyalaya, Anjora, Durg-491 001, Chhattisgarh, India
N.K. Patel
Affiliation:
Department of Parasitology, College of Veterinary Science and Animal Husbandry, Indira Gandhi Krishi Viswavidyalaya, Anjora, Durg-491 001, Chhattisgarh, India
S. Pal
Affiliation:
Department of Parasitology, College of Veterinary Science and Animal Husbandry, Indira Gandhi Krishi Viswavidyalaya, Anjora, Durg-491 001, Chhattisgarh, India
S.C. Mandal
Affiliation:
Department of Parasitology, College of Veterinary Science and Animal Husbandry, Indira Gandhi Krishi Viswavidyalaya, Anjora, Durg-491 001, Chhattisgarh, India
*
*Fax: +91 788 2252139sanyalpk@rediffmail.com
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Abstract

Wild isolates of the egg-parasitic fungi Paecilomyces lilacinus and Verticillium chlamydosporium, obtained from the organic environment of Durg, Chhattisgarh, India, were subjected to screening for in vitro growth using different media types, range of incubation temperature and pH, and their predatory activity to the eggs of Fasciola gigantica and Gigantocotyle explanatum. Maximum growth of P. lilacinus was obtained in corn-meal agar compared to any other media types. The preferred medium for growth of V. chlamydosporium was corn-meal agar, followed by potato-dextrose agar. After initial growth for 16 h of incubation, no growth was observed in water agar for both the fungi. Six different temperatures – 4°C, 10°C, 18°C, 26°C, 34°C and 40°C – were used to observe growth profiles of the fungi in corn-meal agar medium. While no and very little growth of P. lilacinus and V. chlamydosporium was observed at 4°C and 10°C, respectively, growth profiles of both the fungi were optimal at 26–40°C. A range of pH (pH 4–8) supported growth of both P. lilacinus and V. chlamydosporium. Full-grown plates of the fungi baited with viable eggs of F. gigantica and G. explanatum revealed that V. chlamydosporium was more vigorous in its egg-parasitic ability compared to P. lilacinus. Distortion of the eggs started on day 2–3 of egg baiting in culture plates of V. chlamydosporium, with complete distortion by day 7. On the contrary, P. lilacinus exhibited very limited egg-parasitic ability and some of the baited eggs even showed development of miracidia.

Type
Research Papers
Information
Journal of Helminthology , Volume 82 , Issue 3 , September 2008 , pp. 271 - 277
Copyright
Copyright © Cambridge University Press 2008

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