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Seropositivity and identification of paramyosin for sparganosis in the Kangwon and Incheon provinces of the Republic of Korea

Published online by Cambridge University Press:  15 September 2016

M.-R. Lee ,
J.-W. Ju ,
H.-W. Yang ,
T.-S. Kim ,
M.-Y. Park  and
S.-H. Cho
M.-R. Lee
Affiliation:
Division of Malaria and Parasitic Diseases, Center for Immunology and Pathology, National Research Institute of Health, Centers for Disease Control and Prevention, Osong, 28159, Republic of Korea
J.-W. Ju
Affiliation:
Division of Malaria and Parasitic Diseases, Center for Immunology and Pathology, National Research Institute of Health, Centers for Disease Control and Prevention, Osong, 28159, Republic of Korea
H.-W. Yang
Affiliation:
Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine, 101 Dongin-2ga, Jong-gu, Daegu, 70-422, Republic of Korea
T.-S. Kim
Affiliation:
Department of Parasitology, Inha University School of Medicine, Incheon 400-103, Republic of Korea
M.-Y. Park
Affiliation:
Division of Malaria and Parasitic Diseases, Center for Immunology and Pathology, National Research Institute of Health, Centers for Disease Control and Prevention, Osong, 28159, Republic of Korea
S.-H. Cho*
Affiliation:
Division of Malaria and Parasitic Diseases, Center for Immunology and Pathology, National Research Institute of Health, Centers for Disease Control and Prevention, Osong, 28159, Republic of Korea
*
*Fax: +82-43-719-8559 E-mail: malpara2016@gmail.com
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Abstract

Sparganosis is one of the top three tissue-dwelling heterologous helminthic diseases, along with cysticercosis and paragonimiasis, in Korea. Due to a lack of effective early diagnosis and treatment methods, this parasitic disease is regarded as a public health threat. This study evaluated reactivity, against sparganum extracts, of sera from inhabitants of Cheorwon-gun, Goseong-gun and Ongjin-gun in Korea. The sera from 836 subjects were subjected to enzyme-linked immunosorbent assay and immunoblot analysis. The sera from 18 (5.8%) and 15 (5.1%) inhabitants in Cheorwon-gun (n = 312) and Goseong-gun (n = 294), respectively, exhibited highly positive reactions to the sparganum antigen, whereas only two (0.9%) inhabitants in Ongjin-gun (n = 230) showed positivity. We sought antigenic proteins for serodiagnosis of positive sera by immunoproteomic approaches. Total sparganum lysates were separated by two-dimensional electrophoresis and then subjected to immunoblot analysis with mixed sparganosis-positive sera. We found seven antigenic spots and identified paramyosin as an antigenic protein by liquid chromatography–mass spectrometry. By two-dimensional (2D)-based mass analysis and immunoblotting against sparganosis-positive sera, paramyosin was identified as a candidate antigen for serodiagnosis of sparganosis.

Type
Short Communications
Information
Journal of Helminthology , Volume 91 , Issue 5 , September 2017 , pp. 642 - 646
Copyright
Copyright © Cambridge University Press 2016 

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Footnotes

†

These authors contributed equally to this study.

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