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Seroprevalence of toxocariasis in hypereosinophilic individuals in Ahwaz, south-western Iran

Published online by Cambridge University Press:  07 July 2011

S. Maraghi ,
A. Rafiei ,
R. Hajihossein  and
S. M. Sadjjadi
S. Maraghi
Affiliation:
Department of Parasitology and Mycology, Tropical Disease Research Center, Thalassemia and Haemoglobinopathy Research Center, Jundishapur University of Medical Sciences, Ahwaz, Iran
A. Rafiei
Affiliation:
Department of Parasitology and Mycology, Tropical Disease Research Center, Thalassemia and Haemoglobinopathy Research Center, Jundishapur University of Medical Sciences, Ahwaz, Iran
R. Hajihossein
Affiliation:
Department of Parasitology and Mycology, Tropical Disease Research Center, Thalassemia and Haemoglobinopathy Research Center, Jundishapur University of Medical Sciences, Ahwaz, Iran
S. M. Sadjjadi*
Affiliation:
Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
*
*Fax: +987112305291 E-mail: smsadjjadi@sums.ac.ir
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Abstract

Eosinophilia in human peripheral blood is caused by different agents, including toxocariasis. The present study aimed to evaluate the prevalence of toxocariasis in hypereosinophilic individuals in the city of Ahwaz, located in south-western Iran, using enzyme-linked immunosorbent assay and Western blot techniques. Serum samples were examined from 100 individuals with peripheral blood eosinophilia and also from another 100 individuals without eosinophilia as the control group. In hypereosinophilic individuals seroprevalence antibodies against Toxocara were found in 19 (19%), of whom 12 (63.15%) were female and 7 (36.85%) were male. Positive sera were subsequently confirmed by Western blot. All of the observed bands ranged from 24 to 100 kDa. Antibodies against Toxocara were found in 1% of the control group, but were not confirmed by Western blot. The results showed significant differences between the frequency of infection within age and gender (P < 0.05); the highest prevalence of infection was observed in adults. Differences between the hypereosinophilic and healthy individuals, in terms of Toxocara infection frequency, also proved significant (P < 0.05).The present study thus confirmed the significant prevalence of toxocariasis as a hygienic problem among hypereosinophilic individuals in this area. It is, therefore, necessary to examine these individuals for toxocariasis.

Type
Research Papers
Information
Journal of Helminthology , Volume 86 , Issue 2 , June 2012 , pp. 241 - 244
Copyright
Copyright © Cambridge University Press 2011

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References

Ajayi, O.O., Duhlinska, D.D., Agwale, S.M. & Njoku, M. (2000) Frequency of human toxocariasis in Jos, Plateau State, Nigeria. Memórias do Instituto Oswaldo Cruz 95, 147–149.CrossRefGoogle ScholarPubMed
Akao, N. & Ohata, N. (2007) Toxocariasis in Japan. Parasitology International 56, 87–93.CrossRefGoogle ScholarPubMed
Alonso, J.M., Bojanich, M.V., Chamorro, M. & Gorodner, J.O. (2000) Toxocara seroprevalence in children from a subtropical city in Argentina. Revista do Instituto de Medicina Tropical de São Paulo 42, 235–237.CrossRefGoogle ScholarPubMed
Anaruma Filho, F., Chieffi, P.P., Correa, C.R., Camargo, E.D., Silveria, E.P., Aranha, J.J. & Riberio, M.C. (2002) Human toxocariasis: a seroepidemiological survey in the municipality of campinas (SP). Revista do Instituto de Medicina Tropical de São Paulo 44, 303–307.Google Scholar
Azizi, S., Oryan, A., Sadjjadi, S.M. & Zibaei, M. (2007) Gross and histopathologic changes and larval recovery of Toxocara cati in experimentally infected chickens. Parasitology 102, 47–52.CrossRefGoogle Scholar
Baboolal, S. & Rawlins, S.C. (2002) Seroprevalence of toxocariasis in schoolchildren in Trinidad. Transactions of the Royal Society of Tropical Medicine and Hygiene 96, 139–143.Google Scholar
Choi, J.H., Suh, Y.J., Jung, J.W., Song, H.J., Suh, C.H., Huh, S., Nahm, D.H. & Park, H.S. (2003) Clinical significance of serum ECP and sero-prevalence of human toxocariasis in patients with eosinophilia. Asthma Allergy Clinical Immunology 23, 26–32.Google Scholar
Cypess, R.H., Karol, M.H., Zidian, J.L., Glickman, L.T. & Gitlin, D. (1977) Larva specific antibodies in patients with visceral larva migrans. Journal of Infectious Diseases 135, 633–640.Google Scholar
De Andrade Lima Coêlho, R., De Carvalho, L.B. Jr, Perez, E.P., Araki, K., Takeuchi, T., Ito, A., Aoki, T. & Yamasaki, H. (2005) Prevalence of toxocariasis in northeastern Brazil based on serology using recombinant Toxocara canis antigen. American Journal of Tropical Medicine and Hygiene 72, 103–107.Google Scholar
Despommier, D. (2003) Toxocariasis: clinical aspects, epidemiology, medical ecology, and molecular aspects. Clinical Microbiology Review 16, 265–272.Google Scholar
Fan, C.K., Hung, C.C., Du, W.Y., Liao, C.W. & Su, K.E. (2004) Seroepidemiology of Toxocara canis infection among mountain aboriginal schoolchildren living in contaminated districts in eastern Taiwan. Tropical Medicine and International Health 9, 1312–1318.Google Scholar
Gillespie, S.H. (1993) The clinical spectrum of human toxocariasis. pp. 55–56in Lewis, J.W. & Maizel, R.M. (Eds) Toxocara and toxocariasis: Clinical epidemiology and molecular perspectives. London, Institute of Biology and the British Society for Parasitology.Google Scholar
Glickman, L.T. & Schantz, P.M. (1981) Epidemiology and pathogenesis of zoonotic toxocariasis. Epidemiologic Reviews 3, 230.CrossRefGoogle ScholarPubMed
Gueglio, B., de Gentile, L., Nguyen, J.M., Achard, J., Chabasse, D. & Marjolet, M. (2005) Epidemiologic approach to human toxocariasis in Western France. Parasitology Research 80, 531–536.Google Scholar
Herrmann, N., Lawrence, T.G., Glickman, L.T., Schantz, P.M., Weston, M.G. & Domanski, L.M. (1985) Seroprevalence of zoonotic toxocariasis in the United States: 1971–1973. American Journal of Epidemiology 122, 890–896.CrossRefGoogle ScholarPubMed
Kenny, J.V., MacCabe, R.J., Smith, H.V. & Holland, C. (1995) Serological evidence for the presence of toxocariasis in the Turkana District of Kenya. Transactions of the Royal Society of Tropical Medicine and Hygiene 89, 377–378.CrossRefGoogle ScholarPubMed
Kim, Y.H., Huh, S. & Chung, Y.B. (2008) Seroprevalence of toxocariasis among healthy people with eosinophilia. Korean Journal of Parasitology 46, 29–32.CrossRefGoogle ScholarPubMed
Kwon, N.H., Oh, M.J., Lee, S.P., Lee, B.J. & Choi, D.C. (2006) The prevalence and diagnostic value of toxocariasis in unknown eosinophilia. Annals of Hematology 85, 233–238.CrossRefGoogle ScholarPubMed
Logar, J., Soba, B., Kraut, A. & Kranjc, B.S. (2004) Seroprevalence of Toxocara antibodies among patients suspected of ocular toxocariasis in Slovenia. The Korean Journal of Parasitology 42, 137–140.Google Scholar
Lynch, N.R., Wilkes, L.K. & Hodgen, A.N. (1988) Specificity of Toxocara ELISA in tropical populations. Parasite Immunology 10, 323–337.CrossRefGoogle ScholarPubMed
Magnaval, J.F. & Baixench, M.T. (1993) Toxocariasis in the Midi Pyrenees region. pp. 63–69in Lewis, J.W. & Maizel, R.M. (Eds) Toxocara and toxocariasis: Clinical epidemiology and molecular perspectives. London, Institute of Biology and the British Society for Parasitology.Google Scholar
Magnaval, J.F., Fabre, R. & Maurieres, P. (1991) Application of the western-blotting procedure for the immunodiagnosis of human toxocariasis. Parasitology Research 77, 697–702.Google Scholar
Magnaval, J.F., Glickman, L.T., Dorchies, P. & Morassin, B. (2001) Highlights of human toxocariasis. Korean Journal of Parasitology 39, 1–11.Google Scholar
Mohamad, S., Azmi, N.C. & Noordin, R. (2009) Development and evaluation of a sensitive and specific assay for diagnosis of human toxocariasis by use of three recombinant antigens (TES-26, TES-30USM, and TES-120). Journal of Clinical Microbiology 47, 1712–1717.CrossRefGoogle ScholarPubMed
Morimatsu, Y., Akao, N., Akiyoshi, H., Kawazu, T., Okabe, Y. & Aizawa, H. (2006) A familial case of visceral larva migrans after ingestion of raw chicken livers: specific antibody in bronchoalveolar lavage fluid of the patients. American Journal of Tropical Medicine and Hygiene 75, 303–306.Google Scholar
Noordin, R., Smith, H.V., Mohamad, S., Maizels, R.M. & Fong, M.Y. (2005) Comparison of IgG-ELISA and IgG4-ELISA for Toxocara serodiagnosis. Acta Tropica 93, 57–62.Google Scholar
Park, S.P., Park, I., Park, H.Y., Lee, S.U., Huh, S., Kong, Y. & Magnaval, J.F. (2000) Five cases of ocular toxocariasis confirmed by serology. Korean Journal of Parasitology 38, 267–273.CrossRefGoogle ScholarPubMed
Portus, M., Riera, C. & Prats, G. (1989) A serological survey of toxocariasis in patients and healthy donors in Barcelona (Spain). European Journal of Epidemiology 5, 224–227.Google Scholar
Rubinsky-Elefant, G., Hirata, C.E., Yamamoto, J.H. & Ferreira, M.U. (2010) Human toxocariasis: diagnosis, worldwide seroprevalences and clinical expression of the systemic and ocular forms. Annals of Tropical Medicine and Parasitology 104, 3–23.Google Scholar
Sadjjadi, S.M., Khosravi, M., Mehrabani, D. & Oryan, A. (2000) Seroprevalence of Toxocara infection in schoolchildren in Shiraz, southern Iran. Journal of Tropical Pediatrics 46, 327–330.Google Scholar
Smith, H. & Noordin, R. (2006) Diagnostic limitations and future trends in the serodiagnosis of human toxocariasis. pp. 89–112in Holland, C.V. & Smith, H.W. (Eds) Toxocara: The enigmatic parasite. Trowbridge, Cromwell Press.CrossRefGoogle Scholar
Speiser, F. & Gottstein, B. (1984) A collaborative study on larval secretory excretory antigens of Toxocara canis for the immunodiagnosis of human toxocariasis with ELISA. Acta Tropica 41, 361–372.Google Scholar
Thompson, D.E., Bundy, D.A.P., Cooper, E.S. & Schantz, P.M. (1986) Epidemiological characteristics of Toxocara canis zoonotic infection of children in a Caribbean community. Bulletin of the World Health Organization 64, 283–290.Google Scholar
Zibaei, M., Sadjjadi, S.M., Ishyiama, S., Sarkari, B. & Uga, S. (2010) Production of monoclonal antibody against Toxocara cati second stage larvae and its application for the detection of circulating antigens. Hybridoma 29, 217–220.Google Scholar