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Soluble antigens and antibodies in the sera of mice infected with Trichinella spiralis, detected by a modified double counterimmunoelectrophoresis technique

Published online by Cambridge University Press:  18 November 2009

H. V. Smith  and
M. W. Kennedy
M. W. Kennedy
Affiliation:
Department of Zoology, University of Glasgow, Glasgow G12 8QQ, Scotland
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Abstract

Double counterimmunoelectrophoresis, with a novel well format, was used as a sensitive technique for the rapid detection of circulating antigen and antibody in mice infected with Trichinella spiralis. In a primary infection, soluble antigen was initially detected in day 13, followed by a second wave on day 41. Circulating antibody was first apparent on day 34, increasing in intensity and number of precipitation bands until day 102. The first peak of circulating antigen is indicative of non-complexed antigen released from the developing larvae in striated muscle cells, whereas the presence of antigen late in infection is indicative of electrophoresislabile immune complexes. On reinfection (day 35 of primary) circulating antigen was present which could be attributed to products of resident primary infection muscle larvae.

Type
Research Article
Information
Journal of Helminthology , Volume 58 , Issue 1 , March 1984 , pp. 71 - 78
Copyright
Copyright © Cambridge University Press 1984

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