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Use of body fluid of adult female Ascaris suum as an antigen in the enzyme-linked immunosorbent assay (ELISA) for diagnosis of swine ascariosis

Published online by Cambridge University Press:  05 June 2009

S. Yoshihara ,
T. Oya ,
T. Furuya  and
N. Goto
S. Yoshihara
Affiliation:
Kyushu Branch Laboratory, National Institute of Animal Health, Chuzan-cho, Kagoshima-shi, Kagoshima 891-01, Japan
T. Oya
Affiliation:
Kyushu Branch Laboratory, National Institute of Animal Health, Chuzan-cho, Kagoshima-shi, Kagoshima 891-01, Japan
T. Furuya
Affiliation:
Agricultural Technical Center, Pfizer Pharmaceuticals Inc., Kannami-cho, Shizuoka 419-01, Japan
N. Goto
Affiliation:
Department of Safety Research on Biologics, National Institute of Health, Gakuen, Musashimurayama-shi, Tokyo 208, Japan
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Abstract

The Enzyme-linked immunosorbent assay (ELISA) was used to detect Ascaris suum antibodies in swine sera with adult body fluid (ABF) as an antigen. The assay was standardized with respect to the antigen concentration and serum and conjugate dilutions. Cross reaction was found between the antigen and the sera from the swine infected with Metastrongylus apri. The ELISA was more sensitive than the complement fixation test. Five protein peaks were obtained from ABF by gel filtration on Sephacryle S-300. Fraction I was the most specific. A 105 kDa protein in the fraction reacted with swine lgG in the serum of infected animals in Western blot analysis.

Keywords

Ascaris suumadult body fluidELISAWestern blot analysisNematoda
Type
Research Papers
Information
Journal of Helminthology , Volume 67 , Issue 4 , December 1993 , pp. 279 - 286
Copyright
Copyright © Cambridge University Press 1993

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References

REFERENCES

Beer, R. J. S. (1973) Studies on the biology of the life-cycle of Trichuris suis Schrank, 1788. Parasitology, 67, 253–262.Google Scholar
Bennett, C. E. (1978) The identification of soluble adult antigen on the tegument surface of juvenile Fasciola hepatica. Parasitology, 77, 325–332.CrossRefGoogle Scholar
Boulanger, P. & L'ecuyer, C. (1968) Enzootic pneumonia of pigs: Complement-fixation tests for the detection of Mycoplasma antibodies in the serum of immunized rabbits and infected swine. Canadian Journal of Comparative Medicine and Veterinary Science, 32, 547–554.Google Scholar
Cheng, R. W. K. & Ko, R. C. (1991) Cross-reaction between crude antigens of larval Taenia solium (Cysticercus cellulosae) and other helminths of pigs. Veterinary Parasitology, 39, 161–170.Google Scholar
Copeman, D. B. & Gaafar, S. M. (1972) Sequential development of hepatic lesions of ascaridosis in colostrum-deprived pigs. Australian Veterinary Journal, 48, 263–268.Google Scholar
Dunn, D. R. (1955) The culture of earthworms and their infection with Metastrongylus species. The British Veterinary Journal, 111, 97–101.Google Scholar
Eriksen, L., Andersen, S., Nielsen, K., Pedersen, A. & Nielsen, J. (1980) Experimental Ascaris suum infection in pigs. Serological response, eosinophilia in peripheral blood, occurrence of white spots in the liver and worm recovery from the intestine. Nordic Veterinary Medicine, 32, 233–242.Google Scholar
Eriksen, L., Lind, P., Nansen, P., Roepstorff, A. & Urban, J. (1992) Resistance to Ascaris suum in parasite naïve and naturally exposed growers, finishers and sows. Veterinary Parasitology, 41, 137–149.Google Scholar
Genta, R. M., Frei, D. F. & Linke, M. J. (1987) Demonstration and partial characterization of parasite-specific immunoglobulin A responses in human strongyloidiasis. Journal of Clinical Microbiology, 25, 1505–1510.Google Scholar
Glickman, L. T., Grieve, R. B. & Schantz, P. M. (1986) Serologic diagnosis of zoonotic pulmonary dirofilariasis. American Journal of Medicine, 80, 161–164.Google Scholar
Grelck, H., Hörchner, F. & Unterholzner, J. (1981) Zur serologischen Defferenzierung von Ascaris suum und Toxocara canis-Infektion beim Schwein. Zeitschrift für Parasitenkunde, 65, 277–282.CrossRefGoogle Scholar
Kennedy, M. V. & Qureshi, F. (1986) Stage-specific secreted antigens of the parasitic larval stages of the nematode Ascaris. Immunology, 58, 515–522.Google ScholarPubMed
Ko, R. C. & Wong, T. P. (1992) Trichinella spiralis: specificity of ES antigens from pre-encysted larvae. Journal of Helminthology, 66, 38–44.CrossRefGoogle ScholarPubMed
Lowry, L. O., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 192, 265–275.CrossRefGoogle Scholar
Mehl, W. M., Boch, J., Heine, J., Weiland, G. & Henkel, G. (1983) Durch Spulwurmlarven verusachte Leberveränderungen (“milk spots”) bei Schweinen. Berliner und Münchener Tierärzliche Wochenschrift, 96, 405–409.Google Scholar
Northern, C. & Grove, D. I. (1987) Antigenic analysis of Strongyloides ratti infective larvae and adult worms. Immunology and Cell Biology, 65, 231–239.CrossRefGoogle ScholarPubMed
Rhodes, M. B. & Staudinger, L. A. (1983) Antigens in perienteric fluid of Ascaris suum as detected through antibodies in pigs orally inoculated with fully embryonated eggs. Veterinary Parasitology, 12, 179–186.CrossRefGoogle ScholarPubMed
Saeki, H., Suzaki, S., Sei, A., Takahashi, T. & Ishii, T. (1991) Application of ELISA for field survey of milk spots caused by Ascaris suum infection in porkers. Japanese Journal of Parasitology, 40, 374–382.Google Scholar
Taffs, L. F. (1964) Immunological studies on experimental infection of pigs with Ascaris suum Goeze, 1972. III. The antibody response and acquired immunity. Journal of Helminthology, 38, 129–150.Google Scholar
Towbin, H., Staehelin, T. & Gourdon, J. (1979) Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proceedings of the National Academy of Science of the United States of America, 76, 4350–4354.Google Scholar
Urban, J. F Jr. & Romanowski, R. D. (1985) Ascaris suum: Protective immunity in pigs immunized with products from eggs and larvae. Experimental Parasitology, 60, 245–254.Google Scholar
Urban, J. F. Jr, Alizadeh, H. & Romanowski, R. D. (1988) Ascaris suum: Development of intestinal immunity to infective second-stage larvae in swine. Experimental Parasitology, 66, 66–77.CrossRefGoogle ScholarPubMed
Yoshihara, S., Nakagawa, M. & Suda, H. (1987) Detection of complement fixation antibody against Ascaris suum antigen in pigs with white spots in the liver. Japanese Journal of Veterinary Science, 49, 559–561.Google Scholar