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Development and characterisation of an experimental recurrent laryngeal nerve injury model for the study of viral gene therapy

Published online by Cambridge University Press:  25 June 2007

K Fung ,
N D Hogikyan ,
S B Heavner ,
D Ekbom  and
E L Feldman
K Fung*
Affiliation:
Division of Head and Neck Oncology and Reconstructive Surgery, Department of Otolaryngology, University of Western Ontario, London, Ontario, Canada
N D Hogikyan
Affiliation:
Department of Otolaryngology-Head and Neck Surgery Ann Arbor, Michigan, USA
S B Heavner
Affiliation:
Department of Otolaryngology-Head and Neck Surgery Ann Arbor, Michigan, USA
D Ekbom
Affiliation:
Department of Otolaryngology-Head and Neck Surgery Ann Arbor, Michigan, USA
E L Feldman
Affiliation:
Department of Neurology, University of Michigan, Ann Arbor, Michigan, USA
*
Address for correspondence: Dr Kevin Fung, Assistant Professor, Department of Otolaryngology, Division of Head and Neck Oncology and Reconstructive Surgery, London Health Sciences Centre – Victoria Hospital, Room C3-100, 800 Commissioners Road East, London, Ontario, CanadaN6A 4G5. Fax: 519 685 8567 E-mail: kevin.fung@lhsc.on.ca
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Abstract

Objectives:

To develop and characterise an experimental model of recurrent laryngeal nerve injury for the study of viral gene therapy.

Methods:

Twenty rats underwent unilateral recurrent laryngeal nerve injury. After vocal fold mobility was observed, larynges were serially sectioned, and immunohistochemical techniques were employed to stain for neurofilament and motor endplates in order for a blinded investigator to determine the percentage of nerve–endplate contact, as a histological indicator of an intact neuromuscular connection.

Results:

All animal procedures resulted in complete, ipsilateral vocal fold paralysis that recovered by three weeks. The mean nerve–endplate contact percentage was 11.6 per cent at one week, 53.9 per cent at two weeks, 88.6 per cent at three weeks, 81.7 per cent at four weeks and 86.6 per cent at five weeks. The differences between results at week one and week three were statistically significant (p < 0.01). The mean nerve–endplate contact percentage on the control side was 86.8 per cent.

Conclusions:

There was a dramatic, measurable decrease in nerve–endplate contact percentage following crush injury to the recurrent laryngeal nerve. Spontaneous recovery was observed by three weeks post-injury. This model will be used to investigate the potential therapeutic role of viral gene therapy for the treatment of recurrent laryngeal nerve injury.

Keywords

Vocal Cord ParalysisRecurrent Laryngeal NerveGene Therapy
Type
Main Article
Information
The Journal of Laryngology & Otology , Volume 122 , Issue 5 , May 2008 , pp. 500 - 505
Copyright
Copyright © JLO (1984) Limited 2007

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