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Effect of local irradiation with 630 and 860 nm low-level lasers on tympanic membrane perforation repair in guinea pigs

Published online by Cambridge University Press:  21 February 2013

Sh Maleki ,
S K Kamrava ,
D Sharifi ,
M Jalessi ,
A Asghari ,
S Ghalehbaghi  and
P Yazdanifard
Sh Maleki
Affiliation:
Otolaryngology – Head and Neck Surgery Research Center, Rasool Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran
S K Kamrava*
Affiliation:
Otolaryngology – Head and Neck Surgery Research Center, Rasool Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran
D Sharifi
Affiliation:
Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
M Jalessi
Affiliation:
Otolaryngology – Head and Neck Surgery Research Center, Rasool Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran
A Asghari
Affiliation:
Otolaryngology – Head and Neck Surgery Research Center, Rasool Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran
S Ghalehbaghi
Affiliation:
Family Planning and Population Control Department, Islamic Azad University, South Tehran Branch, Tehran, Iran
P Yazdanifard
Affiliation:
Otolaryngology – Head and Neck Surgery Research Center, Rasool Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran
*
Address for correspondence: Dr Seyed Kamran Kamrava, Associate Professor of ENT–Head and Neck Surgery, Otolaryngology-Head and Neck Surgery Research Center, Rasoul Akram Hospital, Tehran University of Medical Sciences, Niayesh St, Sattarkhan Av, Tehran, Iran Fax: +98 21 66552828 E-mail: skamrankamrava@gmail.com
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Abstract

Objective:

In this study, we evaluated the effect of low-level lasers on the healing of tympanic membrane perforation, one of the most common otological pathologies.

Methods and materials:

Twenty-four guinea pigs were randomly assigned to either the experimental or control group. One day after the induction of a 2 mm diameter, centred myringotomy in all animals, the tympanic membranes in the experimental group were irradiated with 630 and 860 nm lasers for 10 days. Two weeks later, histological changes in the membranes were evaluated.

Results:

Tympanic membrane thickening and inflammatory cell infiltration in the tympanic membranes and surrounding tissues were significantly less in the experimental group (p < 0.001). The distance from the external auditory canal wall to the malleus tip did not differ significantly between the two groups (p = 0.42).

Conclusion:

The results show that the combined application of 630 and 860 nm lasers had a significant effect on the healing of tympanic membrane perforation, and on the prevention of thick fibrotic or atelectatic neomembrane formation.

Keywords

EarMiddleOtitis MediaTraumaTympanic MembraneWound HealingLasersGuinea Pigs
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 127 , Issue 3 , March 2013 , pp. 260 - 264
Copyright
Copyright © JLO (1984) Limited 2013

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