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Effects of agmatine sulphate on facial nerve injuries

Published online by Cambridge University Press:  26 January 2017

O Surmelioglu ,
L Sencar ,
S Ozdemir ,
O Tarkan ,
M Dagkiran ,
N Surmelioglu ,
U Tuncer  and
S Polat
O Surmelioglu*
Affiliation:
Department of Otorhinolaryngology, Cukurova University, Adana, Turkey
L Sencar
Affiliation:
Department of Histology, Faculty of Medicine, Cukurova University, Adana, Turkey
S Ozdemir
Affiliation:
Department of Otorhinolaryngology, Cukurova University, Adana, Turkey
O Tarkan
Affiliation:
Department of Otorhinolaryngology, Cukurova University, Adana, Turkey
M Dagkiran
Affiliation:
Department of Otorhinolaryngology, Cukurova University, Adana, Turkey
N Surmelioglu
Affiliation:
Department of Clinical Pharmacy, Faculty of Pharmacy, Cukurova University, Adana, Turkey
U Tuncer
Affiliation:
Department of Otorhinolaryngology, Cukurova University, Adana, Turkey
S Polat
Affiliation:
Department of Histology, Faculty of Medicine, Cukurova University, Adana, Turkey
*
Address for correspondence: Dr Ozgur Surmelioglu, Department of Otorhinolaryngology, Faculty of Medicine, Cukurova University, Adana, Turkey Fax: +90 322 338 6527 E-mail: surmeli2004@yahoo.com
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Abstract

Objective:

To evaluate the effect of agmatine sulphate on facial nerve regeneration after facial nerve injury using electron and light microscopy.

Methods:

The study was performed on 30 male Wistar albino rats split into: a control group, a sham-treated group, a study control group, an anastomosis group, and an anastomosis plus agmatine sulphate treatment group. The mandibular branch of the facial nerve was dissected, and a piece was removed for histological and electron microscopic examination.

Results:

Regeneration was better in the anastomosis group than in the study control group. However, the best regeneration findings were seen in the agmatine sulphate treatment group. There was a significant difference between the agmatine group and the others in terms of median axon numbers (p < 0.004) and diameters (p < 0.004).

Conclusion:

Agmatine sulphate treatment with anastomosis in traumatic facial paralysis may enhance nerve regeneration.

Keywords

Facial NerveAgmatineNerve Regeneration
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 131 , Issue 3 , March 2017 , pp. 221 - 226
Copyright
Copyright © JLO (1984) Limited 2017 

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References

1 Fisch, U. Management of intratemporal facial nerve injuries. J Laryngol Otol 1980;94:129–34Google Scholar
2 Yian, CH, Paniello, RC, Spector, JG. Inhibition of motor nerve regeneration in a rabbit facial nerve model. Laryngoscope 2001;111:786–91CrossRefGoogle Scholar
3 Spector, JG, Lee, P, Derby, A, Roufa, DG. Comparison of rabbit facial nerve regeneration in nerve growth factor-containing silicone tubes to that in autologous neural grafts. Ann Otol Rhinol Laryngol 1995;104:875–85Google Scholar
4 Spector, JG, Lee, P, Derby, A. Rabbit facial nerve regeneration in autologous nerve grafts after antecedent injury. Laryngoscope 2000;110:660–7CrossRefGoogle ScholarPubMed
5 Nachemson, AK, Lundborg, G, Myrhage, R, Rank, F. Nerve regeneration and pharmacological suppression of the scar reaction at the suture site. An experimental study on the effect of estrogen-progesterone, methylprednisolone-acetate and cis-hydroxyproline in rat sciatic nerve. Scand J Plast Reconstr Surg 1985;19:255–60Google Scholar
6 Fu, SY, Gordon, T. Contributing factors to poor functional recovery after delayed nerve repair: prolonged denervation. J Neurosci 1995;15:3886–95CrossRefGoogle ScholarPubMed
7 Gilad, GM, Gilad, VH. Early rapid and transient increase in ornithine decarboxylase activity within sympathetic neurons after axonal injury. Exp Neurol 1983;81:158–66Google Scholar
8 Haas, CA, Donath, C, Kreutzberg, GW. Differential expression of immediate early genes after transection of the facial nerve. Neuroscience 1993;53:91–9Google Scholar
9 Tetzlaff, W, Graeber, MB, Kreutzberg, GW. Ornithine decarboxylase in motoneurons during regeneration. Exp Neurol 1985;89:679–88Google Scholar
10 Dornay, M, Gilad, VH, Shiler, I, Gilad, GM. Early polyamine treatment accelerates regeneration of rat sympathetic neurons. Exp Neurol 1986;92:665–74Google Scholar
11 Gilad, VH, Tetzlaff, WG, Rabey, JM, Gilad, GM. Accelerated recovery following polyamines and aminoguanidine treatment after facial nerve injury in rats. Brain Res 1996;724:141–4CrossRefGoogle ScholarPubMed
12 Oble, DA, Burton, L, Maxwell, K, Hassard, T, Nathaniel, EJ. A comparison of thyroxine- and polyamine-mediated enhancement of rat facial regeneration. Exp Neurol 2004;189:105–11CrossRefGoogle Scholar
13 Regunathan, S, Reis, DJ. Characterization of arginine decarboxylase in rat brain and liver: distinction from ornithine decarboxylase. J Neurochem 2000;74:2201–8Google Scholar
14 Gilad, GM, Gilad, VH, Rabey, JM. Arginine and ornithine decarboxylation in rodent brain: coincidental changes during development and after ischemia. Neurosci Lett 1996;216:33–6Google Scholar
15 Gilad, GM, Salame, K, Rabey, JM, Gilad, VH. Agmatine treatment is neuroprotective in rodent brain injury models. Life Sci 1995;58:41–6CrossRefGoogle Scholar
16 Fairbanks, CA, Schreiber, KL, Brewer, KL, Yu, CG, Stone, LS, Kitto, KF et al. Agmatine reverses pain induced by inflammation, neuropathy, and spinal cord injury. Proc Natl Acad Sci U S A 2000;97:10584–9CrossRefGoogle ScholarPubMed
17 Gilad, GM, Gilad, VH. Accelerated functional recovery and neuroprotection by agmatine after spinal cord ischemia in rats. Neurosci Lett 2000;296:97–100 Google Scholar
18 National Research Council of The National Academies. Guide for the Care and Use of Laboratory Animals, 8th edn. Washington, DC: National Academies Press, 2011 Google Scholar
19 Berenholz, L, Segal, S, Gilad, VH, Klein, C, Yehezkeli, E, Eviatar, E et al. Agmatine treatment and vein graft reconstruction enhance recovery after experimental facial nerve injury. J Peripher Nerv Syst 2005;10:319–28Google Scholar
20 Siemionow, M, Sari, A. A contemporary overview of peripheral nerve research from the Cleveland Clinic microsurgery laboratory. Neurol Res 2004;26:218–25Google Scholar
21 de Faria, SD, Testa, JR, Borin, A, Toledo, RN. Standardization of techniques used in facial nerve section and facial movement evaluation in rats. Braz J Otorhinolaryngol 2006;72:341–7Google Scholar
22 Stoll, G, Muller, HW. Nerve injury, axonal degeneration and neural regeneration: basic insights. Brain Pathol 1999;9:313–25CrossRefGoogle ScholarPubMed
23 Pagnotta, A, Tos, P, Fornaro, M, Gigante, A, Geuna, S, Battiston, B. Neurotrophins and their receptors in early axonal regeneration along muscle-vein-combined grafts. Microsurgery 2002;22:300–3Google Scholar
24 Hirata, K, Kawabuchi, M. Myelin phagocytosis by macrophages and nonmacrophages during Wallerian degeneration. Microsc Res Tech 2002;57:541–7Google Scholar
25 Spector, JG, Lee, P, Peterein, J, Roufa, D. Facial nerve regeneration through autologous nerve grafts: a clinical and experimental study. Laryngoscope 1991;101:537–53CrossRefGoogle ScholarPubMed
26 Pfeiffer, B, Sarrazin, W, Weitzel, G. Insulin-like effects of agmatine derivatives in vitro and in vivo (author's transl) [in German]. Hoppe Seylers Z Physiol Chem 1981;362:1331–7Google Scholar
27 Sun, MK, Regunathan, S, Reis, DJ. Cardiovascular responses to agmatine, a clonidine-displacing substance, in anesthetized rat. Clin Exp Hypertens 1995;17:115–28Google Scholar
28 Raasch, W, Schäfer, U, Chun, J, Dominiak, P. Biological significance of agmatine, an endogenous ligand at imidazoline binding sites. Br J Pharmacol 2001;133:755–80Google Scholar