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Expression of female sex hormone receptors in human middle-ear cholesteatomas

Published online by Cambridge University Press:  08 March 2006

Alexander Markov Raynov ,
Yun-Hoon Choung ,
Sung-Kyun Moon  and
Keehyun Park
Alexander Markov Raynov
Affiliation:
Department of Otorhinolaryngology, University Hospital "Tzaritza Joanna", Medical University – Sofia, Sofia, Bulgaria
Yun-Hoon Choung
Affiliation:
Department of Otolaryngology, Ajou University School of Medicine, Suwon, Korea
Sung-Kyun Moon
Affiliation:
Department of Otolaryngology, Ajou University School of Medicine, Suwon, Korea
Keehyun Park
Affiliation:
Department of Otolaryngology, Ajou University School of Medicine, Suwon, Korea
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Abstract

The purpose of this study was to establish the eventual presence of progesterone receptor (PGR) and oestrogen receptor (EGR) in human middle-ear cholesteatoma (MECh) tissues and to compare their expression between male and female patients. An immunohistochemical technique was employed for detection of PGR- and EGR-specific immunoreactivity in MECh samples using formalin-fixed paraffin-embedded tissue sections. The positive results were verified with reverse transcriptase polymerase chain reaction (RT-PCR). The morphological study revealed stable expression of PGR in suprabasal layers of all cholesteatoma samples. Weaker immunoreactivity for PGR was demonstrated in external auditory canal skin (EACS) samples in comparison with MECh, while PGR-specific staining was not observed in retroauricular skin (RAS) samples. EGR was detected only at mRNA levels. Stronger expression of EGR PCR products was disclosed in female cholesteatoma samples, while PGR mRNA was predominantly detected in male cholesteatoma specimens. Our preliminary experimental results give us ground to assume that female sex hormones may stimulate proliferation and affect differentiation of MECh keratinocytes.

Keywords

Middle EarOestrogen ReceptorsProgesterone ReceptorsCholesteatomaImmunocytochemistry
Type
Research Article
Information
The Journal of Laryngology & Otology , Volume 119 , Issue 12 , December 2005 , pp. 941 - 945
Copyright
© 2005 Royal Society of Medicine Press

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