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Immunological responses against human papilloma virus and human papilloma virus induced laryngeal cancer

Published online by Cambridge University Press:  07 April 2010

S Chitose ,
T Sakazaki ,
T Ono ,
T Kurita ,
H Mihashi  and
T Nakashima
S Chitose*
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, Japan
T Sakazaki
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, Japan
T Ono
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, Japan
T Kurita
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, Japan
H Mihashi
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, Japan
T Nakashima
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, Japan
*
Address for correspondence: Dr Shun-ichi Chitose, Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan. Fax: +81 942 37 1200 E-mail: yonekawa@med.kurume-u.ac.jp
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Abstract

Objective:

This study aimed to clarify the local immune status in the larynx in the presence of infection or carcinogenesis associated with human papilloma virus.

Methods:

Cytological samples (for human papilloma virus detection) and laryngeal secretions (for immunoglobulin assessment) were obtained from 31 patients with laryngeal disease, during microscopic laryngeal surgery. On histological examination, 12 patients had squamous cell carcinoma, four had laryngeal papilloma and 15 had other benign laryngeal disease. Cytological samples were tested for human papilloma virus DNA using the Hybrid Capture 2 assay.

Results:

High risk human papilloma virus DNA was detected in 25 per cent of patients (three of 12) with laryngeal cancer. Low risk human papilloma virus DNA was detected only in three laryngeal papilloma patients. The mean laryngeal secretion concentrations of immunoglobulins M, G and A and secretory immunoglobulin A in human papilloma virus DNA positive patients were more than twice those in human papilloma virus DNA negative patients. A statistically significant difference was observed between the secretory immunoglobulin A concentrations in the two groups. Patients with laryngeal cancer had higher laryngeal secretion concentrations of each immunoglobulin type, compared with patients with benign laryngeal disease. The study assessed the mean laryngeal secretion concentrations of each immunoglobulin type in the 12 laryngeal cancer patients, comparing human papilloma virus DNA positive patients (n = 3) and human papilloma virus DNA negative patients (n = 9); the mean concentrations of immunoglobulins M, G and A and secretory immunoglobulin A tended to be greater in human papilloma virus DNA positive cancer patients, compared with human papilloma virus DNA negative cancer patients.

Conclusion:

These results suggest that the local laryngeal immune response is activated by infection or carcinogenesis due to human papilloma virus. The findings strongly suggest that secretory IgA has inhibitory activity against infection or carcinogenesis associated with human papilloma virus in the larynx.

Keywords

Larynx NeoplasmsHuman Papilloma VirusMucosal Immunity
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 124 , Issue 6 , June 2010 , pp. 659 - 662
Copyright
Copyright © JLO (1984) Limited 2010

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References

1 Chitose, S, Umeno, H, Hamakawa, S, Nakashima, T, Shouji, H. Unilateral associated laryngeal paralysis due to varicella-zoster virus: virus antibody testing and videofluoroscopic findings. J Laryngol Otol 2008;122:170–6CrossRefGoogle ScholarPubMed
2 Mogi, G, Watanabe, N, Maeda, S, Umehara, T. Laryngeal secretions. An immunological and immunohistological study. Acta Otolaryngol (Stockh) 1979;87:129–41CrossRefGoogle Scholar
3 Yonekawa, S, Fukunaga, H, Umeno, H, Mori, K, Nakashima, T. Subglottic airway becomes stable with age in the human infant larynx. Acta Otolaryngol (Stockh) 2000;120:444–9Google ScholarPubMed
4 Nakashima, T, Tomita, H, Chitose, S, Umeno, H, Sato, K. Local immune status and tumour marker expression in the human larynx. J Laryngol Otol 2009;123:1–4CrossRefGoogle Scholar
5 Nakashima, T, Umeno, H, Yonekawa, S, Kikuchi, A, Tsuda, S, Sato, K. Growth of laryngeal glands in infants: a comparative study of the sub- and supraglottic larynx. J Laryngol Otol 2002;116:14–18CrossRefGoogle ScholarPubMed
6 Bransma, JL, Steinberg, BM, Abramson, AL, Winkler, B. Presence of human papilloma virus type 16 related sequence in verrucous carcinoma of the larynx. Cancer Res 1988;46:2185–8Google Scholar
7 Syrjänen, S, Syrjänen, K, Mäntyjärvi, R, Collan, Y, Kärjä, J. Human papilloma virus DNA in squamous cell carcinomas of the larynx demonstrated by in situ hybridization. ORL J Otorhinolaryngol Relat Spec 1987;49:175–86CrossRefGoogle Scholar
8 Kiyabu, MT, Shibata, D, Arnheim, N, Martin, WJ, Fitzgibbons, PL. Detection of human papillomavirus in formalin-fixed, invasive squamous carcinomas using the polymerase chain reaction. Am J Surg Pathol 1989;13:221–4CrossRefGoogle ScholarPubMed
9 Kulmala, SM, Syrjänen, S, Shabalova, I, Petrovichev, N, Kozachenko, V, Podistov, J et al. Human papillomavirus testing with the Hybrid Capture 2 assay and PCR as screening tools. J Clin Microbiol 2004;42:2470–5CrossRefGoogle ScholarPubMed
10 Lörincz, A, Anthony, J. Advances in HPV detection by hybrid capture®. Papillomavirus Report 2001;12:145–54Google Scholar
11 Walboomers, JM, Jacobs, MV, Manos, MM, Bosch, FX, Kummer, JA, Shah, KV et al. Human papillomavirus is a necessary cause of invasive cervical cancer worldwide. J Pathol 1999;189:12–193.0.CO;2-F>CrossRefGoogle ScholarPubMed
12 Mineta, H, Ogino, T, Amano, H, Ohkawa, Y, Araki, K, Takebayashi, S et al. Human papilloma virus type 16 and 18 detected in head and neck squamous cell carcinoma. Cancer Res 1998;18:4765–8Google ScholarPubMed
13 Manjarrez, ME, Ocadiz, R, Valle, L, Pacheco, C, Marroquin, A, De la Torre, C et al. Detection of human papillomavirus and relevant tumor suppressors and oncoproteins in laryngeal tumors. Clin Cancer Res 2006;12:6946–51CrossRefGoogle ScholarPubMed
14 Perez-Ayala, M, Ruiz-Cabello, F, Esteban, F, Concha, A, Redondo, M, Oliva, MR et al. Presence of HPV 16 sequences in laryngeal carcinomas. Int J Cancer 1990;46:8–11CrossRefGoogle ScholarPubMed
15 Brandwein, MS, Nuovo, GJ, Biller, H. Analysis of prevalence of human papillomavirus in laryngeal carcinomas. Study of 40 cases using polymerase chain reaction and consensus primers. Ann Otol Rhinol Laryngol 1993;102:309–13CrossRefGoogle ScholarPubMed
16 Ma, XL, Ueno, K, Pan, ZM, Hi, SZ, Ohyama, M, Eizuru, Y. Human papillomavirus DNA sequences and p53 over-expression in laryngeal squamous cell carcinomas in Northeast China. J Med Virol 1998;54:186–913.0.CO;2-1>CrossRefGoogle ScholarPubMed
17 Melchers, W, van den Brule, A, Walboomers, J, de Bruin, M, Burger, M, Herbrink, P et al. Increased detection rate of human papillomavirus in cervical scrapes by the polymerase chain reaction as compared to modified FISH and southern-blot analysis. J Med Virol 1989;27:329–35CrossRefGoogle ScholarPubMed
18 Kasperbauer, JL, O'Halloran, GL, Espy, MJ, Smith, TF, Lewis, JE. Polymerase chain reaction (PCR) identification of human papillomavirus (HPV) DNA in verrucous carcinoma of the larynx. Laryngoscope 1993;103:416–20CrossRefGoogle ScholarPubMed
19 Nakashima, T, Makishima, K, Komiyama, S, Takeda, K, Hiroto, I. Local immune system in the developing fetal larynx. Laryngoscope 1980;91:398–407CrossRefGoogle Scholar
20 Sasagawa, T, Inoue, M, Lehtinen, M, Zhang, W, Gschmeissner, SE, Hajibagheri, N et al. Serological responses to human papillomavirus type 6 and 16 virus-like particles in patients with cervical neoplastic lesions. Clin Diag Lab Immunol 1996;3:403–10CrossRefGoogle ScholarPubMed
21 Sasagawa, T, Yamazaki, H, Dong, Y, Satake, S, Tateno, M, Inoue, M. Immunoglobulin-A and -G responses against virus-like particles (VLP) of human papillomavirus type 16 in woman with cervical cancer and cervical intra-epithelial lesions. Int J Cancer 1998;75:529–353.0.CO;2-V>CrossRefGoogle Scholar