Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-10T12:46:06.506Z Has data issue: false hasContentIssue false
  • English
  • Français

MicroRNA let-7a up-regulates OPN expression in a mouse model of allergic rhinitis

Published online by Cambridge University Press:  15 August 2017

H Yu ,
H Sun ,
Z Wang  and
Y Liu
H Yu
Affiliation:
Department of Infectious Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P R China
H Sun
Affiliation:
Department of Otorhinolaryngology, Huazhong University of Science and Technology, Wuhan, P R China
Z Wang
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P R China
Y Liu*
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P R China
*
Address for correspondence: Dr Yang Liu, Department of Otolaryngology – Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, P R China E-mail: noah_05@sina.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Objective:

To investigate the effect of microRNA let-7a on OPN expression in a mouse model of allergic rhinitis.

Methods:

Thirty-two mature female C57BL/6 mice were randomly divided into four groups, with eight mice in each group: microRNA let-7a, microRNA control, OVA and phosphate-buffered saline groups. The microRNA let-7a, microRNA control and OVA groups were sensitised with OVA to establish a mouse model of allergic rhinitis. Nose-scratching events were counted. Nasal mucosa was used to evaluate histological changes of goblet cell hyperplasia. Interleukins 5 and 13 were detected. Interferon-γ levels in the nasal lavage fluid were assayed with enzyme-linked immunosorbent assay. OPN expression was estimated with polymerase chain reaction.

Results:

Compared with microRNA control mice, microRNA let-7a treated mice had a significantly increased number of nose-scratching events, nasal mucosal eosinophilia and goblet cell hyperplasia (p < 0.05), and significantly higher interleukins 5 and 13 in nasal mucosa (p < 0.05), but there was no significant difference in interferon-γ (p > 0.05). In addition, microRNA let-7a treated mice had significantly enhanced OPN expression in nasal mucosa (p < 0.05).

Conclusion:

MicroRNA let-7a can promote allergic rhinitis development partly by regulating OPN expression.

Keywords

MicroRNAAllergic RhinitisNasal MucosaOsteopontin
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 131 , Issue 11 , November 2017 , pp. 955 - 960
Copyright
Copyright © JLO (1984) Limited 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1 Greiner, AN, Hellings, PW, Rotiroti, G, Scadding, GK. Allergic rhinitis. Lancet 2011;378:2112–22CrossRefGoogle ScholarPubMed
2 Bousquet, J, Khaltaev, N, Cruz, AA, Denburg, J, Fokkens, WJ, Togias, A et al. Allergic Rhinitis and its Impact on Asthma (ARIA) 2008 update (in collaboration with the World Health Organization, GA2LEN and AllerGen). Allergy 2008;63:8–160 CrossRefGoogle ScholarPubMed
3 Zhang, YM, Zhang, J, Liu, SL, Zhang, X, Yang, SN, Gao, J et al. Prevalence and associated risk factors of allergic rhinitis in preschool children in Beijing. Laryngoscope 2013;123:28–35 CrossRefGoogle ScholarPubMed
4 Rosenwasser, LJ. Current understanding of the pathophysiology of allergic rhinitis. Immunol Allergy Clin North Am 2011;31:433–9CrossRefGoogle ScholarPubMed
5 Ambros, V. MicroRNAs: tiny regulators with great potential. Cell 2001;107:823–6CrossRefGoogle ScholarPubMed
6 Ambros, V. The functions of animal microRNAs. Nature 2004;431:350–5CrossRefGoogle ScholarPubMed
7 Bartel, DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 2004;116:281–97CrossRefGoogle ScholarPubMed
8 Calin, GA, Dumitru, CD, Shimizu, M, Bichi, R, Zupo, S, Noch, E et al. Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia. Proc Natl Acad Sci U S A 2002;99:15524–9Google Scholar
9 Yang, M, Li, Y, Padgett, RW. MicroRNAs: small regulators with a big impact. Cytokine Growth Factor Rev 2005;16:387–93CrossRefGoogle ScholarPubMed
10 Liu, Y, Lu, X, Yu, HJ, Hua, XY, Cui, YH, Huang, SK et al. The expression of osteopontin and its association with Clara cell 10 kDa protein in allergic rhinitis. Clin Exp Allergy 2010;40:1632–41Google Scholar
11 Makino, K, Jinnin, M, Hirano, A, Yamane, K, Eto, M, Kusano, T et al. The downregulation of microRNA let-7a contributes to the excessive expression of type I collagen in systemic and localized scleroderma. J Immunol 2013;190:3905–15CrossRefGoogle Scholar
12 Liu, Y, Yu, HJ, Wang, N, Zhang, YN, Huang, SK, Cui, YH et al. Clara cell 10-kDa protein inhibits T(H)17 responses through modulating dendritic cells in the setting of allergic rhinitis. J Allergy Clin Immunol 2013;131:387–94CrossRefGoogle ScholarPubMed
13 Kim, YH, Yang, TY, Park, CS, Ahn, SH, Son, BK, Kim, JH et al. Anti-IL-33 antibody has a therapeutic effect in a murine model of allergic rhinitis. Allergy 2012;67:183–90CrossRefGoogle Scholar
14 Roggli, E, Britan, A, Gattesco, S, Lin-Marq, N, Abderrahmani, A, Meda, P et al. Involvement of microRNAs in the cytotoxic effects exerted by proinflammatory cytokines on pancreatic beta-cells. Diabetes 2010;59:978–86CrossRefGoogle ScholarPubMed
15 Lu, TX, Munitz, A, Rothenberg, ME. MicroRNA-21 is up-regulated in allergic airway inflammation and regulates IL-12p35 expression. J Immunol 2009;182:4994–5002 CrossRefGoogle ScholarPubMed
16 Lu, TX, Hartner, J, Lim, EJ, Fabry, V, Mingler, MK, Cole, ET et al. MicroRNA-21 limits in vivo immune response-mediated activation of the IL-12/IFN-gamma pathway, Th1 polarization, and the severity of delayed-type hypersensitivity. J Immunol 2011;187:3362–73CrossRefGoogle ScholarPubMed
17 Shell, S, Park, SM, Radjdai, AR, Schickel, R, Kistner, EO, Jewell, DA et al. Let-7 expression defines two differentiation stages of cancer. Proc Natl Acad Sci USA 2007;104:11400–5CrossRefGoogle ScholarPubMed
18 Polikepahad, S, Knight, JM, Naghavi, AO, Oplt, T, Creighton, CJ, Shaw, C et al. Proinflammatory role for let-7 microRNAS in experimental asthma. J Biol Chem 2010;285:30139–49CrossRefGoogle ScholarPubMed
19 Cantor, H, Shinohara, ML. Regulation of T-helper-cell lineage development by osteopontin: the inside story. Nat Rev Immunol 2009;9:137–41CrossRefGoogle ScholarPubMed