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Novel connexin 30 and connexin 26 mutational spectrum in patients with progressive sensorineural hearing loss

Published online by Cambridge University Press:  15 June 2012

S Battelino ,
B Repič Lampret ,
M Žargi  and
K Trebušak Podkrajšek
S Battelino
Affiliation:
Department of Otorhinolaryngology and Cervicofacial Surgery, University Medical Centre Ljubljana, Slovenia
B Repič Lampret
Affiliation:
Centre for Medical Genetics, University Children's Hospital, University Medical Centre Ljubljana, Slovenia
M Žargi
Affiliation:
Department of Otorhinolaryngology and Cervicofacial Surgery, University Medical Centre Ljubljana, Slovenia
K Trebušak Podkrajšek*
Affiliation:
Centre for Medical Genetics, University Children's Hospital, University Medical Centre Ljubljana, Slovenia
*
Address for correspondence: Dr Katarina Trebušak Podkrajšek, University Medical Centre Ljubljana, University Children's Hospital, Bohoričeva 20, SI-1252 Ljubljana, Slovenia Fax: +386 1 522 93 57 E-mail: katarina.trebusak@kclj.si
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Abstract

Objective:

Mutations in the gap junction protein beta-2 gene (‘GJB2’) are known to be responsible for mild to profound congenital and late-onset hearing loss. This study aimed to investigate the molecular basis of progressive hearing loss compared with non-progressive hearing loss.

Methods:

Following clinical otorhinolaryngological evaluation, a genetic analysis was performed in a cohort of 72 patients with progressive sensorineural hearing loss.

Results:

Pathological genotypes were established in 16 patients (22.2 per cent). Six different gap junction protein beta-2 gene mutations were detected in 15 patients, with the c.35delG mutation responsible for 56 per cent of the mutated alleles. A novel gap junction protein beta-6 gene (‘GJB6’) mutation (p.Met203Val) was observed in one patient with mild progressive hearing loss.

Conclusion:

Analyses of gap junction protein beta-2 and -6 genes revealed that similar pathological genotypes, occurring with similar frequencies, were responsible for progressive hearing loss, compared with reported genotypes for non-progressive hearing loss patients. Thus, genotype cannot be used to differentiate non-progressive from progressive hearing loss cases; in this study, patients both with and without an established pathological genotype had a similar clinical course.

Keywords

Hearing Loss, SensorineuralGeneticsConnexin 26Connexin 30
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 126 , Issue 8 , August 2012 , pp. 763 - 769
Copyright
Copyright © JLO (1984) Limited 2012

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