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Single dose intratympanic mesna application inhibits propylene glycol induced cholesteatoma formation

Published online by Cambridge University Press:  20 December 2016

O Ismi ,
Y Y Karabulut ,
K K Bal ,
Y Vayisoglu  and
M Unal
O Ismi*
Affiliation:
Department of Otorhinolaryngology, Faculty of Medicine, University of Mersin, Turkey
Y Y Karabulut
Affiliation:
Department of Pathology, Faculty of Medicine, University of Mersin, Turkey
K K Bal
Affiliation:
Department of Otorhinolaryngology, Faculty of Medicine, University of Mersin, Turkey
Y Vayisoglu
Affiliation:
Department of Otorhinolaryngology, Faculty of Medicine, University of Mersin, Turkey
M Unal
Affiliation:
Department of Otorhinolaryngology, Faculty of Medicine, University of Mersin, Turkey
*
Address for correspondence: Dr O Ismi, Mersin Üniversitesi Tıp Fakültesi Kulak Burun Boğaz A D, Çiftlikköy, Mezitli, Mersin, Turkey E-mail: dronurismi@gmail.com
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Abstract

Objective:

Mesna (i.e. sodium 2-mercaptoethanesulfonate; C2H5NaO3S2) has been used in otological surgery such as cholesteatoma dissection and tympanic membrane lateralisation in atelectatic ears. However, this study aimed to investigate its effect on cholesteatoma formation.

Methods:

A total of 20 Wistar rats were divided into two groups of 10 animals. The right and left ears of control animals were treated with saline (saline control group; n = 10 ears) and propylene glycol plus saline (propylene glycol control group; n = 10 ears), respectively. In the mesna group, both ears were treated with propylene glycol plus mesna (n = 20 ears). On days 1, 8 and 15, the saline control group had intratympanic injections of 0.2 ml saline and the propylene glycol control and mesna groups had intratympanic injections of 0.2 ml 100 per cent propylene glycol. On day 22, the propylene glycol control group had a single intratympanic injection of 0.2 ml saline and the mesna group had a single intratympanic injection of 10 per cent mesna. Animals were killed 12 weeks after the last injection and the temporal bones were sent for histopathological evaluation.

Results:

The cholesteatoma formation rate was 88 per cent in the propylene glycol control group, but was significantly lower in the mesna group (p = 0.01). There were no significant differences in granulation tissue formation (p = 0.498), cyst formation in the bulla (p = 0.381), fibrosis (p = 0.072) and epithelial hyperplasia (p = 0.081) among experimental groups.

Conclusion:

Intratympanic propylene glycol administration is an effective method of promoting experimental cholesteatoma formation. Administration of a single dose of intratympanic mesna inhibited cholesteatoma formation in an animal model.

Keywords

MesnaCholesteatomaPropylene GlycolOtitis Media
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 131 , Issue 3 , March 2017 , pp. 215 - 220
Copyright
Copyright © JLO (1984) Limited 2016 

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