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Cell origin in the macula flava of the human newborn vocal fold

Published online by Cambridge University Press:  06 May 2016

K Sato ,
S Chitose ,
T Kurita  and
H Umeno
K Sato*
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, Japan
S Chitose
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, Japan
T Kurita
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, Japan
H Umeno
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, Japan
*
Address for correspondence: Dr Kiminori Sato, Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan Fax: +81 942 37 1200 E-mail: kimisato@oct-net.ne.jp
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Abstract

Background:

There is growing evidence to suggest that cells in the maculae flavae are tissue stem cells of the human vocal fold and maculae flavae are a stem cell niche.

Methods:

Three newborn vocal folds were investigated. Immunoreactivity to antibodies directed to cytokeratin, desmin, glial fibrillary acidic protein, vimentin, cluster of differentiation 34, cluster of differentiation 45, collagen type I, telomerase reverse transcriptase, SOX17 and stage-specific embryonic antigen 3 was investigated.

Results:

The cells in the newborn maculae flavae expressed haematopoietic markers (cluster of differentiation 34, cluster of differentiation 45) and collagen type I, which are the major makers of bone marrow derived circulating fibrocytes. The cells expressed epithelium, muscle, neural and mesenchymal cell associated proteins, and endodermal marker, indicating that they are undifferentiated and express proteins of all three germ layers. The cells also expressed stage-specific embryonic antigen 3 and telomerase reverse transcriptase.

Conclusion:

The cells in the newborn maculae flavae are undifferentiated cells arising from the differentiation of bone marrow cells. The results of this study are consistent with the hypothesis that the cells in maculae flavae are tissue stem cells.

Keywords

Vocal CordStem CellsAdult Stem CellsStem Cell NicheNewbornLarynx
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 130 , Issue 7 , July 2016 , pp. 650 - 655
Copyright
Copyright © JLO (1984) Limited 2016 

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Footnotes

Presented (and awarded Poster Presentation Second Place Award) at the 95th Annual Meeting of the American Broncho-Esophagological Association, 22–23 April 2015, Boston, Massachusetts, USA.

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