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Chemiluminescence assay of reactive oxygen species in laryngeal cancer

Published online by Cambridge University Press:  20 May 2010

T Baglam ,
M Sari ,
Z Mine Yazici ,
M Yuksel  and
C Uneri
T Baglam*
Affiliation:
Department of Otolaryngology Head Neck Surgery, School of Medicine, Gaziantep University, Turkey
M Sari
Affiliation:
Department of Otolaryngology Head Neck Surgery, School of Medicine, Marmara University, Istanbul, Turkey
Z Mine Yazici
Affiliation:
Department of Otolaryngology Head Neck Surgery, School of Medicine, Marmara University, Istanbul, Turkey
M Yuksel
Affiliation:
Vocational School of Health Related Sciences, Marmara University, Istanbul, Turkey
C Uneri
Affiliation:
Department of Otolaryngology Head Neck Surgery, School of Medicine, Marmara University, Istanbul, Turkey
*
Address for correspondence: Dr Tekin Baglam, Sahinbey Uygulama ve Arastırma Hastanesi, Kulak Burun Bogaz AD, 27100 Sahinbey, Gaziantep, Turkey. E-mail: tekinbaglam@hotmail.com
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Abstract

Objective:

This study aimed to evaluate the presence of reactive oxygen species in laryngeal cancer tissue, using a luminol-amplified chemiluminescence method.

Materials and methods:

Fourteen patients with histopathologically diagnosed laryngeal squamous cell carcinoma were enrolled. Patients with recurrent tumours or a history of prior chemotherapy or radiotherapy were excluded. Tissue specimens were harvested both from the tumour itself and from the neighbouring, apparently normal mucosa (immediately after tumour removal). Tissue specimens were washed with ice-cold saline solution and processed immediately, without storage. The level of reactive oxygen species was measured quantitatively by a luminol-amplified chemiluminescence method.

Results:

The mean luminol-amplified chemiluminescence values for tumour and control tissue were 140.52 (standard error of the mean 40.21) and 121.36 (standard error of the mean 35.33) relative light units/mg tissue, respectively. Furthermore, mean tumour and control luminol chemiluminescence values were compared for stage one and two tumours versus stage three and four tumours. Both the tumour and the control luminol chemiluminescence values for the latter tumour group were significantly higher than those for the former tumour group.

Conclusion:

This study measured directly the levels of reactive oxygen species in samples of laryngeal cancer tissue and normal mucosa. Higher levels of reactive oxygen species were found in laryngeal cancer tissue, suggesting a relationship between reactive oxygen species and laryngeal cancer.

Keywords

Larynx CancerReactive Oxygen SpeciesChemiluminescenceLuminol
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 124 , Issue 10 , October 2010 , pp. 1091 - 1094
Copyright
Copyright © JLO (1984) Limited 2010

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