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Differentiation of adipose-derived stem cells into ear auricle cartilage in rabbits

Published online by Cambridge University Press:  12 June 2012

H Bahrani ,
M Razmkhah ,
M J Ashraf ,
N Tanideh ,
N Chenari ,
B Khademi  and
A Ghaderi
H Bahrani
Affiliation:
Department of ENT, School of Medicine, Shiraz University of Medical Sciences, Iran
M Razmkhah
Affiliation:
Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Iran
M J Ashraf
Affiliation:
Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Iran
N Tanideh
Affiliation:
Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Iran
N Chenari
Affiliation:
Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Iran
B Khademi
Affiliation:
Department of ENT, School of Medicine, Shiraz University of Medical Sciences, Iran
A Ghaderi*
Affiliation:
Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Iran Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Iran
*
Address for correspondence: Dr Abbas Ghaderi, Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, PO Box 71345-1798, Shiraz, Iran Fax: 0098 711 230 4952 E-mail: ghaderia@sums.ac.ir
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Abstract

Background:

Adipose-derived stem cells have been reported as a novel candidate for the repair of cartilage injuries in vivo.

Methods:

In order to assess their differentiation ability, adipose-derived stem cells isolated from rabbit fat tissue were injected into the midportion of a surgically created rabbit ear auricle cartilage defect. After several months, the auricles were resected, histopathologically assessed and compared with a control group.

Results:

Histopathological examination of auricles removed three, four and five months after injection showed islands of new cartilage formation at the site of the surgically induced defect. Six months after injection, we observed well-formed, mature cartilaginous plates that completely filled the defect in the native cartilage. In the control group, there was no significant growth of new cartilage.

Conclusion:

The results of this study suggest the great potential of adipose-derived stem cells to repair damaged cartilage tissue in vivo.

Keywords

Mesenchymal Stem CellsAdipose TissueCartilageRegenerative Medicine
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 126 , Issue 8 , August 2012 , pp. 770 - 774
Copyright
Copyright © JLO (1984) Limited 2012

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References

1 Raghunath, J, Salasinski, H, Sales, K, Butler, PE, Seifalian, AM. Advancing cartilage tissue engineering: the application of stem cell technology. Curr Opin Biotechnol 2005;16:503–9CrossRefGoogle ScholarPubMed
2 Robey, PG, Bianco, P. The use of adult stem cells in rebuilding the human face. J Am Dent Assoc 2006;137:961–72CrossRefGoogle ScholarPubMed
3 Zuk, PA, Zhu, M, Ashjian, P, De Ugarte, DA, Huang, JI, Mizuno, H et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 2002;13:4279–95CrossRefGoogle ScholarPubMed
4 Yamamoto, N, Akamatsu, H, Hasegawa, S, Yamada, T, Nakata, S, Ohkuma, M et al. Isolation of multipotent stem cells from mouse adipose tissue. J Dermatol Sci 2007;48:43–52 CrossRefGoogle ScholarPubMed
5 Dominici, M, Hofmann, T, Horwitz, E. Bone marrow mesenchymal cells: biological properties and clinical applications. J Biol Regul Homeost Agents 2001;15:28–37 Google ScholarPubMed
6 Bianco, P, Riminucci, M, Gronthos, S, Robey, P. Bone marrow stromal stem cells: nature, biology, and potential applications. Stem Cells 2001;19:180–92CrossRefGoogle ScholarPubMed
7 Csaki, C, Schneider, PR, Shakibaei, M. Mesenchymal stem cells as a potential pool for cartilage tissue engineering. Ann Anat 2008;190:395–412 CrossRefGoogle ScholarPubMed
8 Toghraie, FS, Chenari, N, Gholipour, MA, Faghih, Z, Torabinejad, S, Dehghani, S et al. Treatment of osteoarthritis with infrapatellar fat pad derived mesenchymal stem cells in rabbit. Knee 2011;18:71–5CrossRefGoogle ScholarPubMed
9 Park, KH, Na, K. Effect of growth factors on chondrogenic differentiation of rabbit mesenchymal cells embedded in injectable hydrogels. J Biosci Bioeng 2008;106:74–9CrossRefGoogle ScholarPubMed
10 Nöth, U, Rackwitz, L, Heymer, A, Weber, M, Baumann, B, Steinert, A et al. Chondrogenic differentiation of human mesenchymal stem cells in collagen type 1 hydrogels. J Biomed Mater Res A 2007;83:626–35CrossRefGoogle ScholarPubMed
11 Wakitani, S, Okabe, T, Horibe, S, Mitsuoka, T, Saito, M, Koyama, T et al. Safety of autologous bone marrow-derived mesenchymal stem cell transplantation for cartilage repair in 41 patients with 45 joints followed for up to 11 years and 5 months. J Tissue Eng Regen Med 2011;5:146–50CrossRefGoogle Scholar
12 Richardson, SM, Hoyland, JA, Mobasheri, R, Csaki, C, Shakibaei, M, Mobasheri, A. Mesenchymal stem cells in regenerative medicine: opportunities and challenges for articular cartilage and intervertebral disc tissue engineering. J Cell Physiol 2010;222:23–32 CrossRefGoogle ScholarPubMed