Hostname: page-component-78c5997874-g7gxr Total loading time: 0 Render date: 2024-11-10T07:20:18.412Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of local use of l-carnitine after myringotomy on myringosclerosis development in rats

Published online by Cambridge University Press:  03 April 2013

E Vuralkan ,
S Alicura Tokgöz ,
G Simsek ,
F Koybasioglu ,
U Han ,
M Caliskan ,
O Besalti  and
I Akin
E Vuralkan*
Affiliation:
Department of Otorhinolaryngology Clinic, Trabzon Kanuni Research and Training Hospital, Ankara, Turkey
S Alicura Tokgöz
Affiliation:
Department of Otorhinolaryngology (Clinic I), Diskapi Yildirim Beyazit Research and Training Hospital, Ankara, Turkey
G Simsek
Affiliation:
Department of Otorhinolaryngology (Clinic I), Diskapi Yildirim Beyazit Research and Training Hospital, Ankara, Turkey
F Koybasioglu
Affiliation:
Department of Pathology (Clinic I), Diskapi Yildirim Beyazit Research and Training Hospital, Ankara, Turkey
U Han
Affiliation:
Department of Pathology (Clinic I), Diskapi Yildirim Beyazit Research and Training Hospital, Ankara, Turkey
M Caliskan
Affiliation:
Faculty of Veterinary Medicine, Ankara University, Turkey
O Besalti
Affiliation:
Faculty of Veterinary Medicine, Ankara University, Turkey
I Akin
Affiliation:
Department of Otorhinolaryngology (Clinic I), Diskapi Yildirim Beyazit Research and Training Hospital, Ankara, Turkey
*
Address for correspondence: Dr Erkan Vuralkan, Department of Otorhinolaryngology Clinic, Trabzon Kanuni Research and Training Hospital, Kaşüstü Mevkii, Yomra, Trabzon, Turkey61290 Fax: +90 4622302307 E-mail: erkanvuralkan@hotmail.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Objectives:

This study aimed to investigate the effect of local and intraperitoneal administration of l-carnitine on the prevention of experimentally induced myringosclerosis, and to compare treatment efficiency.

Methods:

Twenty-four Albino-Wistar rats (48 ears) were bilaterally myringotomised and divided randomly into four groups: group one received no treatment, group two received intraperitoneal l-carnitine, group three received local l-carnitine, and group four received both intraperitoneal and local l-carnitine. On the 15th day after treatment, tympanic membranes were harvested and evaluated histopathologically for myringosclerotic plaque formation, fibroblastic proliferation, tympanic membrane thickness and new vessel formation.

Results:

The group one tympanic membranes showed extensive thickness, and the incidence of myringosclerosis and fibroblast proliferation were greater than in groups two and four. There were statistically significant differences in tympanic membrane thickness between groups three and four, and in myringosclerosis incidence and fibroblast proliferation, comparing groups two, three and four.

Conclusion:

Myringosclerosis development was significantly reduced in rats receiving myringotomy plus intraperitoneal l-carnitine. Intraperitoneal l-carnitine administration prevented fibroblastic proliferation and tympanic membrane thickening (both of which cause further tympanic membrane destruction), thus reducing myringotomy-associated morbidity. Local l-carnitine administration had limited effectiveness in this experimental setting.

Keywords

l-carnitineMyringosclerosisTympanic MembraneRatsInflammation
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 127 , Issue 5 , May 2013 , pp. 468 - 472
Copyright
Copyright © JLO (1984) Limited 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1Tos, M, Stangerup, SE. Hearing loss in tympanosclerosis caused by grommets. Arch Otolaryngol Head Neck Surg 1989;115:931–5CrossRefGoogle ScholarPubMed
2Tos, M, Bonding, P, Poulsen, G. Tympanosclerosis of the drum in secretory otitis after insertion of grommets. A prospective, comparative study. J Laryngol Otol 1983;97:489–96CrossRefGoogle ScholarPubMed
3Mattsson, C, Marklund, SL, Hellström, S. Application of oxygen free radical scavengers to diminish the occurrence of myringosclerosis. Ann Otol Rhinol Laryngol 1997;106:513–8CrossRefGoogle ScholarPubMed
4Spratley, JE, Hellstrom, SO, Mattsson, CK, Pais-Clemente, M. Topical ascorbic acid reduces myringosclerosis in perforated tympanic membranes. A study in the rat. Ann Otol Rhinol Laryngol 2001;110:585–91CrossRefGoogle ScholarPubMed
5Ozcan, C, Gorur, K, Cinel, L, Talas, DU, Unal, M, Cinel, I. The inhibitory effect of topical N-acetylcysteine application on myringosclerosis in perforated rat tympanic membrane. Int J Pediatr Otorhinolaryngol 2002;63:179–84CrossRefGoogle ScholarPubMed
6Ozcan, C, Polat, G, Gorur, K, Talas, DU, Bagdatoglu, O, Cinel, I. The effect of local administration of N-acetylcysteine in perforated rat tympanic membrane: an experimental study in myringosclerosis. Pharmacol Res 2002;45:5–9CrossRefGoogle ScholarPubMed
7Fabriello, RG, Calabrase, F. Prevention of ischemia induced increase in MDA by acetyl carnitine. Ann Neurol 1988;24:114–8Google Scholar
8Flanagan, JL, Simmons, PA, Vehige, J, Willcox, MD, Garrett, Q. Role of carnitine in disease. Nutr Metab (Lond) 2010;16:7–30Google Scholar
9Mattsson, C, Magnuson, K, Hellström, S. Myringosclerosis caused by increased oxygen concentration in traumatized tympanic membranes. Experimental study. Ann Otol Rhinol Laryngol 1995;104:625–32CrossRefGoogle ScholarPubMed
10Song, JJ, Kwon, SK, Cho, CG, Park, SW. The effect of caffeic acid phenethyl ester on the prevention of experimentally induced myringosclerosis. Int J Pediatr Otorhinolaryngol 2007;71:1287–91CrossRefGoogle ScholarPubMed
11Akbaş, Y, Pata, YS, Gorur, K, Polat, G, Polat, A, Ozcan, C et al. The effect of L-carnitine on the prevention of experimentally induced myringosclerosis in rats. Hear Res 2003;184:107–12CrossRefGoogle ScholarPubMed
12Emir, H, Kaptan, ZK, Samim, E, Sungu, N, Ceylan, K, Ustun, H. The preventive effect of ginkgo biloba extract in myringosclerosis: study in rats. Otolaryngol Head Neck Surg 2009;140:171–6CrossRefGoogle ScholarPubMed
13Gorur, K, Ozcan, C, Polat, A, Unal, M, Tamer, L, Cinel, I. The anti-oxidant and anti-apoptotic activities of selenium in the prevention of myringosclerosis in rats. J Laryngol Otol 2002;116:426–9CrossRefGoogle ScholarPubMed
14Güneri, EA, Tekin, S, Yilmaz, O, Ozkara, E, Erdağ, TK, Ikiz, AO et al. The effects of hyaluronic acid, epidermal growth factor, and mitomycin in an experimental model of acute traumatic tympanic membrane perforation. Otol Neurotol 2003;24:371–6CrossRefGoogle Scholar
15Johnson, AP, Smallman, LA, Kent, SE. The mechanism of healing of tympanic membrane perforations. A two-dimensional histological study in guinea pigs. Acta Otolaryngol 1990;109:406–15CrossRefGoogle ScholarPubMed
16Ovesen, T, Gaihede, M, Ledet, T. The effect of N-acetylcysteine on the in vitro growth of normal rabbit middle ear fibroblasts. Clin Otolaryngol Allied Sci 1993;18:400–5CrossRefGoogle ScholarPubMed
17Mayes, PA. Lipids of physiologic significance. In: Murray, RK, Granner, DK, Mayes, PA, Rodwell, VW, eds. Harper's Biochemistry, 25th edn.New York: Mercurant-Hall, 2000;160–71Google Scholar