Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-10T11:20:05.766Z Has data issue: false hasContentIssue false
  • English
  • Français

Epstein-Barr virus BHRF1 prohibits the cells of nasopharyngeal carcinoma from apoptosis

Published online by Cambridge University Press:  29 June 2007

H. Huang ,
J. H. Zhou ,
S. M. Zhou ,
J. H. Hu ,
X. H. Pan ,
X. T. Kong ,
L. Yu ,
X. Y. Sun  and
W. Wu
H. Huang*
Affiliation:
Department of Otolaryngology, Changhai Hospital, Institute of Genetics, Shanghai 200433, People's Republic of China.
J. H. Zhou
Affiliation:
Department of Pharmacology, Changhai Hospital, Institute of Genetics, Shanghai 200433, People's Republic of China.
S. M. Zhou
Affiliation:
Department of Otolaryngology, Changhai Hospital, Institute of Genetics, Shanghai 200433, People's Republic of China.
J. H. Hu
Affiliation:
Department of Pharmacology, Changhai Hospital, Institute of Genetics, Shanghai 200433, People's Republic of China.
X. H. Pan
Affiliation:
Department of Biology, The Second Military Medical University, Shanghai 200433, People's Republic of China.
X. T. Kong
Affiliation:
Department of Central Laboratory of Clinical Immunology, General Military, Changzheng Hospital, The Second Military Medical University, Shanghai 200433, People's Republic of China.
L. Yu
Affiliation:
Department of Central Laboratory of Clinical Immunology, General Military, Changzheng Hospital, The Second Military Medical University, Shanghai 200433, People's Republic of China.
W. Wu
Affiliation:
Department of Otolaryngology, Changhai Hospital, Institute of Genetics, Shanghai 200433, People's Republic of China.
*
Address for correspondence: Hai Huang, Department of Otolaryngology, Changhai Hospital, Second Military Medical University, Shanghai, 200433, People's Republic of China.
Get access Rights & Permissions [Opens in a new window]

Abstract

Epstein-Barr virus (EBV) is associated with nasopharyngeal carcinoma (NPC). The BHRF1 EBV protein is expressed at high levels in productively infected cells and certain latently infected cells. In order to investigate the effect of expression of BHRF1 on the biological behaviour of NPC cells, we constructed the BHRF1 high expression vector and transfected it into the NPC cell line, CNE2. Then, the alteration of proliferation and apoptotic rates in the cells were tested before and after camptothecin treatment. After treatment by camptothecin, BHRF1-CNE2 cells could constantly and slowly proliferate and its apoptotic rate was less than in control groups, and the number of cells in the G phase decreased and in the S phase increased. So, it suggests that BHRF1 expression can enhance the resistibility of CNE2 cells to DNA damaging agents that cause apoptosis.

Keywords

Epstein-Barr virusNasopharyngeal neoplasmsCarcinomaCamptothecinGenesviral
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 111 , Issue 12 , December 1997 , pp. 1147 - 1150
Copyright
Copyright © JLO (1984) Limited 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Cleary, M. L., Smith, S. D., Sklar, J. (1986) Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/ immunoglobulin transcript resulting from the t(14;18) translocation. Cell 47: 19–28.CrossRefGoogle Scholar
Dawson, C. W., Eliopoulos, A. G., Dawson, J., Young, L. S. (1995) BHRF1, a viral homologue of the Bcl-2 oncogene, disturbs epithelial cell differentiation. Oncogene 9: 69–77.Google Scholar
Heller, M., Dambaugh, T., Kieff, E. (1981) Epstein-Barr virus DNAIX variation among viral DNAs from producer and nonproducer infected cells. Journal of Virology 38: 632–648.CrossRefGoogle ScholarPubMed
Henderson, S., Huen, D., Rowe, M., Dawson, G., Johnson, G., Rickinson, A. (1993) Epstein-Barr virus-coded BHRF1 protein, a viral homologue of bcl-2, protects human B cells from programmed cell death. Proceedings of the National Academy of Sciences of the USA 90: 8479–8483.CrossRefGoogle Scholar
Hickish, T. (1994) Ultrastructural localization of BHRF1: an Epstein-Barr virus gene product which has homology with bcl-2. Cancer Research 54: 2808–2811.Google ScholarPubMed
Hockenbery, D., Nunez, G., Milliman, C., Schreiber, R. D., Korsmeyer, S. J. (1990) Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature (London) 348: 334–336.CrossRefGoogle ScholarPubMed
McDonnell, T. J., Nunez, G., Platt, F. M., Hockenberry, D., London, L., McKearn, J. P., Korsmeyer, S. J. (1990) Deregulated bcl-2-immunoglobulin transgene expands a resting but responsive immunoglobulin M- and D-expressing B-cell population. Molecular & Cellular Biology 10: 1901–1907.Google ScholarPubMed
Nunez, G., Seto, M., Seremetis, S., Ferrero, D., Grignani, F., Korsmeyer, S. J., Dalla-Favera, R. (1989) Growth and tumor-promoting effects of deregulated BCL2 in human B-lymphoblastoid cells. Proceedings of the National Academy of Sciences of the USA 86: 4589–4593.CrossRefGoogle ScholarPubMed
Reed, J. C., Haldar, S., Cuddy, M. P., Croce, C., Makover, D. (1989) Deregulated BCL2 expression enhances growth of a human B cell line. Oncogene 4: 1123–1127.Google ScholarPubMed
Shimizu, T., Pommier, Y. (1996) DNA fragmentation induced by protease activation in p53-null human leukemia HL60 cells undergoing apoptosis following treatment with the topoisomerase I inhibitor camptothecin: cell-free system studies. Experimental Cell Research 226: 292–301.CrossRefGoogle ScholarPubMed
Tarodi, B., Subramanian, T., Chinnadurai, G. (1994) Epstein-Barr virus BHRF1 protein protects against cell death induced by DNA-damaging agents and heterologous viral infection. Virology 201: 404–407.CrossRefGoogle ScholarPubMed
Theodorakis, P., D'Sa-Eipper, C., Subramanian, T., Chinnadurai, G. (1996) Unmasking of a proliferation-restraining activity of the anti-apoptosis protein EBV BHRF1. Oncogene 12: 1707–1713.Google ScholarPubMed
Traganos, F., Kapuscinski, J., Gong, J., Ardelt, B., Darzynkiewicz, R. J., Darzynkiewicz, Z. (1993) Caffeine prevents apoptosis and cell cycle effects induced by camptothecin or topotecan in HL-60 cells. Cancer Research 53: 4613–4618.Google ScholarPubMed
Tsujimoto, Y. (1989) Stress-resistance conferred by high level of bcl-2-alpha protein in human B lymphoblastoid cell. Oncogene 4: 1331–1336.Google ScholarPubMed
Walton, M. I., Whysong, D., Connor, P. M., Hockenbery, D., Korsmeyer, S. J., Kohn, K. W. (1993) Constitutive expression of human Bcl-2 modulates nitrogen mustard and camptothecin induced apoptosis. Cancer Research 53: 1853–1861.Google ScholarPubMed